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International Journal of Molecular Medicine
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Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells

  • Authors:
    • Chengrui Nan
    • Yunpeng Shi
    • Zongmao Zhao
    • Shucheng Ma
    • Jixiang Liu
    • Dongdong Yan
    • Guoqiang Song
    • Hanjie Liu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, The First Hospital of Handan City, Handan, Hebei 056000, P.R. China
    Copyright: © Nan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1057-1062
    |
    Published online on: August 10, 2015
       https://doi.org/10.3892/ijmm.2015.2307
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Abstract

In the present study, human umbilical cord-derived mesenchymal stem cells (hUMSCs) were investigated for their potential to be induced to differentiate in vitro into neuron-like cells by monosialoteterahexosyl ganglioside (GM1). Mononuclear cells obtained from umbilical cords from women with full-term pregnancies whose babies were delivered by cesarean section were cultivated in vitro and their surface antigen expression profiles were monitored. Following amplification, the cells were divided into 5 groups, of which 4 (groups A-D) were treated with GM1 at doses of 50, 100, 150 and 200 µg/ml, respectively. The control (group E) was treated with the vehicle only. The ability of GM1 to induce the differentiation of the hUMSCs into neuron-like cells was monitored for 6 h. The expression levels of microtubule-associated protein-2 (MAP-2), neurofilament protein (NF-H) and glial fibrillary acidic protein (GFAP) were measured by immunohistochemistry. Following exposure to GM1, the hUMSCs first appeared to have a diamond or polygonal shape and gradually grew into long spindle-shaped cells, finally exhibiting a radiating or swirling pattern. The cells maintained a strong proliferative capacity after continuous passage. Flow cytometry revealed that the hUMSCs expressed CD73, CD90 and CD105 up to passage 10, but not CD11b, CD19, CD34, CD45 or HLA-DR. Treatment with GM1 for 6 h led to the appearance of neuron-like cells with oval-shaped cell bodies and protruding neurites. These neuron-like cells were positive for MAP-2 and NF-H, but negative for GFAP expression. No changes in the expression of these markers were observed in the control group. Thus, the findings of the present study demonstrate that GM1 effectively induces hUMSCs to differentiate into neuron-like cells.
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Copy and paste a formatted citation
Spandidos Publications style
Nan C, Shi Y, Zhao Z, Ma S, Liu J, Yan D, Song G and Liu H: Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells. Int J Mol Med 36: 1057-1062, 2015.
APA
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D. ... Liu, H. (2015). Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells. International Journal of Molecular Medicine, 36, 1057-1062. https://doi.org/10.3892/ijmm.2015.2307
MLA
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D., Song, G., Liu, H."Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells". International Journal of Molecular Medicine 36.4 (2015): 1057-1062.
Chicago
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D., Song, G., Liu, H."Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells". International Journal of Molecular Medicine 36, no. 4 (2015): 1057-1062. https://doi.org/10.3892/ijmm.2015.2307
Copy and paste a formatted citation
x
Spandidos Publications style
Nan C, Shi Y, Zhao Z, Ma S, Liu J, Yan D, Song G and Liu H: Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells. Int J Mol Med 36: 1057-1062, 2015.
APA
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D. ... Liu, H. (2015). Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells. International Journal of Molecular Medicine, 36, 1057-1062. https://doi.org/10.3892/ijmm.2015.2307
MLA
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D., Song, G., Liu, H."Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells". International Journal of Molecular Medicine 36.4 (2015): 1057-1062.
Chicago
Nan, C., Shi, Y., Zhao, Z., Ma, S., Liu, J., Yan, D., Song, G., Liu, H."Monosialoteterahexosyl ganglioside induces the differentiation of human umbilical cord-derived mesenchymal stem cells into neuron-like cells". International Journal of Molecular Medicine 36, no. 4 (2015): 1057-1062. https://doi.org/10.3892/ijmm.2015.2307
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