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International Journal of Molecular Medicine
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October-2015 Volume 36 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Article

Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion

  • Authors:
    • Wenjuan Wu
    • Xizhi Zhang
    • Houning Lv
    • Yuexia Liao
    • Weicheng Zhang
    • Haichao Cheng
    • Zijing Deng
    • Jingyuan Shen
    • Qing Yuan
    • Yu Zhang
    • Weigan Shen
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China, Clinical Medical College, Yangzhou University, Yangzhou, Jiangsu 225001, P.R. China
  • Pages: 1104-1110
    |
    Published online on: August 11, 2015
       https://doi.org/10.3892/ijmm.2015.2310
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Abstract

Human immediate early response 2 (IER2) has been characterized as a putative nuclear protein that functions as a transcription factor or transcriptional co‑activator in the regulation of cellular responses, and may be involved in the regulation of tumor progression and metastasis. Data from our previous gene expression profile of the human microvascular endothelial cells during capillary morphogenesis showed a significant alteration of IER2 expression, suggesting that IER2 may participate in the regulation of the endothelial cell morphogenesis and angiogenesis. The aim of the present study was to investigate the role of IER2 in cell motility, cell‑matrix adhesion and in vitro capillary‑like structures formation of the human umbilical vein endothelium cells (HUVECs). IER2 was constitutively expressed in HUVECs, and lentiviral‑mediated depletion of IER2 significantly reduced the cell motility, cell‑matrix adhesion and capillary‑like structures formation of HUVECs. Results also showed that depletion and overexpression of IER2 altered the actin cytoskeleton rearrangement in HUVECs. Furthermore, results from western blot analysis showed that the activity of the focal adhesion kinase (FAK) can be regulated by IER2. These results indicated that IER2 regulates endothelial cell motility, adhesion on collagen type I matrix and the capillary tube formation, as the result of the regulation of the actin cytoskeleton rearrangement presumably via a FAK‑dependent mechanism.
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View References

1 

Folkman J: Role of angiogenesis in tumor growth and metastasis. Semin Oncol. 29(Suppl 16): 15–18. 2002. View Article : Google Scholar

2 

Birdsey GM, Dryden NH, Amsellem V, Gebhardt F, Sahnan K, Haskard DO, Dejana E, Mason JC and Randi AM: Transcription factor Erg regulates angiogenesis and endothelial apoptosis through VE-cadherin. Blood. 111:3498–3506. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Iruela-Arispe ML and Dvorak HF: Angiogenesis: A dynamic balance of stimulators and inhibitors. Thromb Haemost. 78:672–677. 1997.PubMed/NCBI

4 

Cabrita MA, Jones LM, Quizi JL, Sabourin LA, McKay BC and Addison C: Focal adhesion kinase inhibitors are potent anti-angiogenic agents. Mol Oncol. 5:517–526. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T and Utiyama H: Expression of a novel immediate early gene during 12-O-tetradecanoylphorbol-13-acetate-induced macrophagic differentiation of HL-60 cells. J Biol Chem. 266:12157–12161. 1991.PubMed/NCBI

6 

Deng YJ, Huang ZX, Zhou CJ, Wang JW, You Y, Song ZQ, Xiang MM, Zhong BY and Hao F: Gene profiling involved in immature CD4+ T lymphocyte responsible for systemic lupus erythematosus. Mol Immunol. 43:1497–1507. 2006. View Article : Google Scholar

7 

Shen QY and Zheng SS: Identification of genes differentially expressed in monocyte-derived dendritic cells with 1á, 25-dihydroxyvitamin D3 using cDNA arrays. J Zhejiang Univ SCI. 5:222–225. 2004. View Article : Google Scholar

8 

Zeng F, Hon CC, Sit WH, Chow KY, Hui RK, Law IK, Ng VW, Yang XT, Leung FC and Wan JM: Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray. Int J Oncol. 27:513–523. 2005.PubMed/NCBI

9 

Takaya T, Kasatani K, Noguchi S and Nikawa J: Functional analyses of immediate early gene ETR101 expressed in yeast. Biosci Biotechnol Biochem. 73:1653–1660. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Chen L, Ma S, Li B, Fink T, Zachar V, Takahashi M, Cuttichia J, Tsui LC, Ebbesen P and Liu X: Transcriptional activation of immediate-early gene ETR101 by human T-cell leukaemia virus type I Tax. J Gen Virol. 84:3203–3214. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J and Klos A: The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes. Arthritis Rheum. 44:2392–2401. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Hong SK and Dawid IB: FGF-dependent left-right asymmetry patterning in zebrafish is mediated by Ier2 and Fibp1. Proc Natl Acad Sci USA. 106:2230–2235. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Neeb A, Wallbaum S, Novac N, Dukovic-Schulze S, Scholl I, Schreiber C, Schlag P, Moll J, Stein U and Sleeman JP: The immediate early gene Ier2 promotes tumor cell motility and metastasis, and predicts poor survival of colorectal cancer patients. Oncogene. 31:3796–3806. 2012. View Article : Google Scholar

14 

Bayless KJ and Davis GE: The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices. J Cell Sci. 115:1123–1136. 2002.PubMed/NCBI

15 

Sun XT, Zhang MY, Shu C, Li Q, Yan XG, Cheng N, Qiu YD and Ding YT: Differential gene expression during capillary morphogenesis in a microcarrier-based three-dimensional in vitro model of angiogenesis with focus on chemokines and chemokine receptors. World J Gastroenterol. 11:2283–2290. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Kuo MW, Wang CH, Wu HC, Chang SJ and Chuang YJ: Soluble THSD7A is an N-glycoprotein that promotes endothelial cell migration and tube formation in angiogenesis. PLoS One. 6:e290002011. View Article : Google Scholar : PubMed/NCBI

17 

Otrock ZK, Mahfouz RA, Makarem JA and Shamseddine AI: Understanding the biology of angiogenesis: Review of the most important molecular mechanisms. Blood Cells Mol Dis. 39:212–220. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Kubota Y, Kleinman HK, Martin GR and Lawley TJ: Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J Cell Biol. 107:1589–1598. 1988. View Article : Google Scholar : PubMed/NCBI

19 

Braren R, Hu H, Kim YH, Beggs HE, Reichardt LF and Wang R: Endothelial FAK is essential for vascular network stability, cell survival, and lamellipodial formation. J Cell Biol. 172:151–162. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Ettenson DS and Gotlieb AI: Endothelial wounds with disruption in cell migration repair primarily by cell proliferation. Microvasc Res. 48:328–337. 1994. View Article : Google Scholar : PubMed/NCBI

21 

Eccles SA: Parallels in invasion and angiogenesis provide pivotal points for therapeutic intervention. Int J Dev Biol. 48:583–598. 2004. View Article : Google Scholar : PubMed/NCBI

22 

Carmeliet P: Angiogenesis in health and disease. Nat Med. 9:653–660. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Fraley SI, Feng Y, Krishnamurthy R, Kim DH, Celedon A, Longmore GD and Wirtz D: A distinctive role for focal adhesion proteins in three-dimensional cell motility. Nat Cell Biol. 12:598–604. 2010. View Article : Google Scholar : PubMed/NCBI

24 

van Nimwegen MJ and van de Water B: Focal adhesion kinase: A potential target in cancer therapy. Biochem Pharmacol. 73:597–609. 2007. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Wu W, Zhang X, Lv H, Liao Y, Zhang W, Cheng H, Deng Z, Shen J, Yuan Q, Zhang Y, Zhang Y, et al: Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion. Int J Mol Med 36: 1104-1110, 2015.
APA
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H. ... Shen, W. (2015). Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion. International Journal of Molecular Medicine, 36, 1104-1110. https://doi.org/10.3892/ijmm.2015.2310
MLA
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H., Deng, Z., Shen, J., Yuan, Q., Zhang, Y., Shen, W."Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion". International Journal of Molecular Medicine 36.4 (2015): 1104-1110.
Chicago
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H., Deng, Z., Shen, J., Yuan, Q., Zhang, Y., Shen, W."Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion". International Journal of Molecular Medicine 36, no. 4 (2015): 1104-1110. https://doi.org/10.3892/ijmm.2015.2310
Copy and paste a formatted citation
x
Spandidos Publications style
Wu W, Zhang X, Lv H, Liao Y, Zhang W, Cheng H, Deng Z, Shen J, Yuan Q, Zhang Y, Zhang Y, et al: Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion. Int J Mol Med 36: 1104-1110, 2015.
APA
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H. ... Shen, W. (2015). Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion. International Journal of Molecular Medicine, 36, 1104-1110. https://doi.org/10.3892/ijmm.2015.2310
MLA
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H., Deng, Z., Shen, J., Yuan, Q., Zhang, Y., Shen, W."Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion". International Journal of Molecular Medicine 36.4 (2015): 1104-1110.
Chicago
Wu, W., Zhang, X., Lv, H., Liao, Y., Zhang, W., Cheng, H., Deng, Z., Shen, J., Yuan, Q., Zhang, Y., Shen, W."Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion". International Journal of Molecular Medicine 36, no. 4 (2015): 1104-1110. https://doi.org/10.3892/ijmm.2015.2310
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