Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
October-2015 Volume 36 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October-2015 Volume 36 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species

  • Authors:
    • Hong‑Li Yu
    • Teng‑Fei Zhao
    • Hao Wu
    • Yao‑Zong Pan
    • Qian Zhang
    • Kui‑Long Wang
    • Chen‑Chao Zhang
    • Yang‑Ping Jin
  • View Affiliations / Copyright

    Affiliations: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, P.R. China
  • Pages: 1127-1135
    |
    Published online on: August 19, 2015
       https://doi.org/10.3892/ijmm.2015.2315
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Pinellia ternata (PT) is a widely used traditional Chinese medicine. The raw material has a throat-irritating toxicity that is associated with the PT lectin (PTL). PTL is a monocot lectin isolated from the tubers of PT, which exhibits mouse peritoneal acute inflammatory effects in vivo. The present study aimed to investigate the pro-inflammatory effect of PTL on macrophages. PTL (50 µg/ml)‑stimulated macrophages enhanced the chemotactic activity of neutrophils. PTL (50, 100, 200 and 400 µg/ml) significantly elevated the production of cytokines [tumor necrosis factor‑α (TNF-α) , interleukin (IL)‑1β and IL‑6]. PTL (25, 50 and 100 µg/ml) induced intracellular reactive oxygen species (ROS) overproduction. PTL also caused transfer of p65 from the macrophage cytoplasm to the nucleus and activated the nuclear factor‑κB (NF‑κB) signaling pathway. Scanning electron microscope images revealed severe cell swelling and membrane integrity defection of macrophages following PTL (100 µg/ml) stimulation, which was also associated with inflammation. PTL had pro‑inflammatory activity, involving induced neutrophil migration, cytokine release, ROS overproduction and the activation of the NF-κB signaling pathway, which was associated with the activation of macrophages.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Peumans WJ and Van Damme EJ: Lectins as plant defense proteins. Plant Physiol. 109:347–352. 1995. View Article : Google Scholar : PubMed/NCBI

2 

Moreira RA, Ainouz IL, De Oliveira JT and Cavada BS: Plant lectins, chemical and biological aspects. Mem Inst Oswaldo Cruz. 86(Suppl 2): 211–218. 1991. View Article : Google Scholar

3 

Cavada BS, Santos CF, Grangeiro TB, Nunes EP, Sales PV, Ramos RL, De Sousa FA, Crisostomo CV and Calvete JJ: Purification and characterization of a lectin from seeds of Vatairea macrocarpa Duke. Phytochemistry. 49:675–680. 1998. View Article : Google Scholar : PubMed/NCBI

4 

Gabius HJ and Gabius S: Glycosciences: Status and perspectives. Wiley-VCH; New York: pp. 6592002

5 

Alencar VB, Brito GA, Alencar NM, Assreuy AM, Pinto VP, Teixeira EH, Souza EP, Debray H, Ribeiro RA and Cavada BS: Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells. Biochem Cell Biol. 83:659–666. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Mota MR, Criddle DN, Alencar NM, Gomes RC, Meireles AV, Santi-Gadelha T, Gadelha CA, Oliveira CC, Benevides RG, Cavada BS, et al: Modulation of acute inflammation by a chitin-binding lectin from Araucaria angustifolia seeds via mast cells. Naunyn Schmiedebergs Arch Pharmacol. 374:1–10. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Santos-de-Oliveira R, Dias-Baruffi M, Thomaz SM, Beltramini LM and Roque-Barreira MC: A neutrophil migration-inducing lectin from Artocarpus integrifolia. J Immunol. 153:1798–1807. 1994.PubMed/NCBI

8 

Assreuy AM, Shibuya MD, Martins GJ, De Souza ML, Cavada BS, Moreira RA, Oliveira JT, Ribeiro RA and Flores CA: Anti-inflammatory effect of glucose-mannose binding lectins isolated from Brazilian beans. Mediators Inflamm. 6:201–210. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Alencar NM, Teixeira EH, Assreuy AM, Cavada BS, Flores CA and Ribeiro RA: Leguminous lectins as tools for studying the role of sugar residues in leukocyte recruitment. Mediators Inflamm. 8:107–113. 1999. View Article : Google Scholar

10 

Cavada BS, Barbosa T, Arruda S, Grangeiro TB and Barral-Netto M: Revisiting proteus: Do minor changes in lectin structure matter in biological activity? Lessons from and potential biotechnological uses of the Diocleinae subtribe lectins. Curr Protein Pept Sci. 2:123–135. 2001. View Article : Google Scholar

11 

Alencar NM, Assreuy AM, Alencar VB, Melo SC, Ramos MV, Cavada BS, Cunha FQ and Ribeiro RA: The galactose-binding lectin from Vatairea macrocarpa seeds induces in vivo neutrophil migration by indirect mechanism. Int J Biochem Cell Biol. 35:1674–1681. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Assreuy AM, Alencar NM, Cavada BS, Rocha-Filho DR, Feitosa RF, Cunha FQ, Calvete JJ and Ribeiro RA: Porcine spermadhesin PSP-I/PSP-II stimulates macrophages to release a neutrophil chemotactic substance: Modulation by mast cells. Biol Reprod. 68:1836–1841. 2003. View Article : Google Scholar : PubMed/NCBI

13 

Alencar NM, Assreuy AM, Criddle DN, Souza EP, Soares PM, Havt A, Aragão KS, Bezerra DP, Ribeiro RA and Cavada BS: Vatairea macrocarpa lectin induces paw edema with leukocyte infiltration. Protein Pept Lett. 11:195–200. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Alencar VB, Assreuy AM, Alencar NM, Meireles AV, Mota MR, Aragão KS, Cajazeiras JB, Nagano CS, Brito GA, Silva LI, et al: Lectin of Pisum arvense seeds induces in vivo and in vitro neutrophil migration. J Pharm Pharmacol. 57:375–381. 2005. View Article : Google Scholar : PubMed/NCBI

15 

McEver RP: Leukocyte-endothelial cell interactions. Curr Opin Cell Biol. 4:840–849. 1992. View Article : Google Scholar : PubMed/NCBI

16 

Cronstein BN and Weissmann G: The adhesion molecules of inflammation. Arthritis Rheum. 36:147–157. 1993. View Article : Google Scholar : PubMed/NCBI

17 

Malik AB and Lo SK: Vascular endothelial adhesion molecules and tissue inflammation. Pharmacol Rev. 48:213–229. 1996.PubMed/NCBI

18 

Alencar NM, Assreuy AM, Havt A, Benevides RG, de Moura TR, de Sousa RB, Ribeiro RA, Cunha FQ and Cavada BS: Vatairea macrocarpa (Leguminosae) lectin activates cultured macrophages to release chemotactic mediators. Naunyn Schmiedebergs Arch Pharmacol. 374:275–282. 2007. View Article : Google Scholar

19 

Liu XQ, Wu H, Yu HL, Zhao TF, Pan YZ and Shi RJ: Purification of a lectin from Arisaema erubescens (Wall.) Schott and its pro-inflammatory effects. Molecules. 16:9480–9494. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Yu HL, Zhu FG and Wu G: Toxic proteins on Raphides from Pinellia ternata and Pinellia pedatisecta. Chin J Tradit Chin Med Pharm. 26:1037–1042. 2011.

21 

Sawant KV, Cho H, Lyons M, Ly LH and McMurray DN: Guinea pig neutrophil-macrophage interactions during infection with Mycobacterium tuberculosis. Microbes Infect. 12:828–837. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Sánchez-Fidalgo S, da Silva MS, Cárdeno A, Aparicio-Soto M, Salvador MJ, Frankland Sawaya AC, Souza-Brito AR and de la Lastra CA: Abarema cochliacarpos reduces LPS-induced inflammatory response in murine peritoneal macrophages regulating ROS-MAPK signal pathway. J Ethnopharmacol. 149:140–147. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Kim MK, Chung SW, Kim DH, Kim JM, Lee EK, Kim JY, Ha YM, Kim YH, No JK, Chung HS, et al: Modulation of age-related NF-kappaB activation by dietary zingerone via MAPK pathway. Exp Gerontol. 45:419–426. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Shangary S, Singh J, Kamboj SS, Kamboj KK and Sandhu RS: Purification and properties of four monocot lectins from the family Araceae. Phytochemistry. 40:449–455. 1995. View Article : Google Scholar : PubMed/NCBI

25 

Anstead GM, Chandrasekar B, Zhang Q and Melby PC: Multinutrient undernutrition dysregulates the resident macrophage proinflammatory cytokine network, nuclear factor-kappaB activation, and nitric oxide production. J Leukoc Biol. 74:982–991. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Silva MT: When two is better than one: Macrophages and neutrophils work in concert in innate immunity as complementary and cooperative partners of a myeloid phagocyte system. J Leukoc Biol. 87:93–106. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Alencar VB, Alencar NM, Assreuy AM, Mota ML, Brito GA, Aragão KS, Bittencourt FS, Pinto VP, Debray H, Ribeiro RA, et al: Pro-inflammatory effect of Arum maculatum lectin and role of resident cells. Int J Biochem Cell Biol. 37:1805–1814. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Gloire G, Legrand-Poels S and Piette J: NF-kappaB activation by reactive oxygen species: Fifteen years later. Biochem Pharmacol. 72:1493–1505. 2006. View Article : Google Scholar : PubMed/NCBI

29 

Kim YS, Morgan MJ, Choksi S and Liu ZG: TNF-induced activation of the Nox1 NADPH oxidase and its role in the induction of necrotic cell death. Mol Cell. 26:675–687. 2007. View Article : Google Scholar : PubMed/NCBI

30 

Han J, Zhong CQ and Zhang DW: Programmed necrosis: Backup to and competitor with apoptosis in the immune system. Nat Immunol. 12:1143–1149. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Kung G, Konstantinidis K and Kitsis RN: Programmed necrosis, not apoptosis, in the heart. Circ Res. 108:1017–1036. 2011. View Article : Google Scholar : PubMed/NCBI

32 

McCall K: Genetic control of necrosis - another type of programmed cell death. Curr Opin Cell Biol. 22:882–888. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yu HL, Zhao TF, Wu H, Pan YZ, Zhang Q, Wang KL, Zhang CC and Jin YP: Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species. Int J Mol Med 36: 1127-1135, 2015.
APA
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K. ... Jin, Y. (2015). Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species. International Journal of Molecular Medicine, 36, 1127-1135. https://doi.org/10.3892/ijmm.2015.2315
MLA
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K., Zhang, C., Jin, Y."Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species". International Journal of Molecular Medicine 36.4 (2015): 1127-1135.
Chicago
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K., Zhang, C., Jin, Y."Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species". International Journal of Molecular Medicine 36, no. 4 (2015): 1127-1135. https://doi.org/10.3892/ijmm.2015.2315
Copy and paste a formatted citation
x
Spandidos Publications style
Yu HL, Zhao TF, Wu H, Pan YZ, Zhang Q, Wang KL, Zhang CC and Jin YP: Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species. Int J Mol Med 36: 1127-1135, 2015.
APA
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K. ... Jin, Y. (2015). Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species. International Journal of Molecular Medicine, 36, 1127-1135. https://doi.org/10.3892/ijmm.2015.2315
MLA
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K., Zhang, C., Jin, Y."Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species". International Journal of Molecular Medicine 36.4 (2015): 1127-1135.
Chicago
Yu, H., Zhao, T., Wu, H., Pan, Y., Zhang, Q., Wang, K., Zhang, C., Jin, Y."Pinellia ternata lectin exerts a pro-inflammatory effect on macrophages by inducing the release of pro-inflammatory cytokines, the activation of the nuclear factor-κB signaling pathway and the overproduction of reactive oxygen species". International Journal of Molecular Medicine 36, no. 4 (2015): 1127-1135. https://doi.org/10.3892/ijmm.2015.2315
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team