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International Journal of Molecular Medicine
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October-2015 Volume 36 Issue 4

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Article

Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells

  • Authors:
    • Tae‑Rin Kwon
    • Chang Taek Oh
    • Eun Ja Choi
    • Hye Min Park
    • Hae Jung Han
    • Hyi Jeong Ji
    • Beom Joon Kim
  • View Affiliations / Copyright

    Affiliations: Major in Biomedical Science, Department of Medicine, Graduate School, Chung‑Ang University, Seoul 156‑755, Republic of Korea, Green Cross Corporation, Giheung‑gu, Yongin-si, Gyeonggi-do 446-770, Republic of Korea
  • Pages: 1088-1096
    |
    Published online on: August 19, 2015
       https://doi.org/10.3892/ijmm.2015.2316
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Abstract

Human placental extract (HPE) is widely used in Korea to relieve fatigue. However, its effects on human dermal papilla cells (hDPCs) remain unknown. In the present study, in an effort to develop novel therapies to promote hair growth, we screened HPE. We demonstrate that HPE has hair growth‑promoting activities and induces β‑catenin expression through the inhibition of glycogen synthase kinase‑3β (GSK‑3β) by phosphorylation in hDPCs. Treatment with HPE significantly increased the viability of the hDPCs in a concentration‑dependent manner, as shown by bromodeoxyuridine (BrdU) assay. HPE also significantly increased the alkaline phosphatase (ALP) expression levels. The increased β‑catenin levels and the inhibition of GSK‑3β (Ser9) by phosphorylation suggested that HPE promoted the hair-inductive capacity of hDPCs. We compared the effects of treatment with HPE alone and treatment with HPE in conjunction with minoxidil (MXD). We found that HPE plus MXD effectively inhibited GSK‑3β by phosphorylation (Ser9) in the hDPCs. Moreover, we demonstrated that HPE was effective in inducing root hair elongation in rat vibrissa hair follicles, and that treatment with HPE led to a delay in catagen progression. Overall, our findings suggest that HPE promotes hair growth and may thus provide the basis of a novel therapeutic strategy for the clinical treatment of hair loss.
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Copy and paste a formatted citation
Spandidos Publications style
Kwon TR, Oh CT, Choi EJ, Park HM, Han HJ, Ji HJ and Kim BJ: Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells. Int J Mol Med 36: 1088-1096, 2015.
APA
Kwon, T., Oh, C.T., Choi, E.J., Park, H.M., Han, H.J., Ji, H.J., & Kim, B.J. (2015). Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells. International Journal of Molecular Medicine, 36, 1088-1096. https://doi.org/10.3892/ijmm.2015.2316
MLA
Kwon, T., Oh, C. T., Choi, E. J., Park, H. M., Han, H. J., Ji, H. J., Kim, B. J."Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells". International Journal of Molecular Medicine 36.4 (2015): 1088-1096.
Chicago
Kwon, T., Oh, C. T., Choi, E. J., Park, H. M., Han, H. J., Ji, H. J., Kim, B. J."Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells". International Journal of Molecular Medicine 36, no. 4 (2015): 1088-1096. https://doi.org/10.3892/ijmm.2015.2316
Copy and paste a formatted citation
x
Spandidos Publications style
Kwon TR, Oh CT, Choi EJ, Park HM, Han HJ, Ji HJ and Kim BJ: Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells. Int J Mol Med 36: 1088-1096, 2015.
APA
Kwon, T., Oh, C.T., Choi, E.J., Park, H.M., Han, H.J., Ji, H.J., & Kim, B.J. (2015). Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells. International Journal of Molecular Medicine, 36, 1088-1096. https://doi.org/10.3892/ijmm.2015.2316
MLA
Kwon, T., Oh, C. T., Choi, E. J., Park, H. M., Han, H. J., Ji, H. J., Kim, B. J."Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells". International Journal of Molecular Medicine 36.4 (2015): 1088-1096.
Chicago
Kwon, T., Oh, C. T., Choi, E. J., Park, H. M., Han, H. J., Ji, H. J., Kim, B. J."Human placental extract exerts hair growth-promoting effects through the GSK-3β signaling pathway in human dermal papilla cells". International Journal of Molecular Medicine 36, no. 4 (2015): 1088-1096. https://doi.org/10.3892/ijmm.2015.2316
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