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Article

Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands

  • Authors:
    • Hyunkeun Song
    • Yeonye Kim
    • Gabin Park
    • Yeong-Seok Kim
    • Seonghan Kim
    • Hyun-Kyung Lee
    • Woo Yeong Chung
    • Seok Ju Park
    • Sang-Youb Han
    • Daeho Cho
    • Daeyoung Hur
  • View Affiliations / Copyright

    Affiliations: Department of Microbiology and Immunology, Laboratory for Medical Oncology, Inje University College of Medicine, Busan 614‑735, Republic of Korea, Department of Anatomy, Inje University College of Medicine, Busan 614‑735, Republic of Korea, Department of Internal Medicine, Inje University Busan Paik Hospital, Busan 614‑735, Republic of Korea, Department of Pediatrics, Inje University Busan Paik Hospital, Busan 614‑735, Republic of Korea, Department of Internal Medicine, Inje University Ilsan-Paik Hospital, Goyang, Gyeonggi 411‑706, Republic of Korea, Department of Life Science, Sookmyung Women's University, Yongsan-ku, Seoul 140-742, Republic of Korea
  • Pages: 1180-1188
    |
    Published online on: August 20, 2015
       https://doi.org/10.3892/ijmm.2015.2317
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Abstract

Transforming growth factor-β (TGF-β) has a significant role in the response to injury and tissue repair, and it has been detected in various cell types. However, the mechanism by which it regulates the response to ischemia‑reperfusion injury (IRI) and manipulates natural killer (NK) cells is not well understood. In the present study, TGF‑β modulated NK cell function, thereby promoting recovery from renal IRI. Human renal proximal tubular epithelial cells (HK‑2) treated with TGF‑β exhibited increased surface and intracellular expression of the NK group 2 member D (NKG2D) ligand MICA. This increased surface expression of MICA inhibited NK cell cytotoxicity to the HK‑2 cells. In addition, an enzyme‑linked immunosorbent assay revealed that TGF‑β treatment evidently increased the amount of soluble MICA released into the culture supernatant from HK‑2 cells. Taken together, these findings suggest that TGF‑β‑induced release of soluble MICA leads to downregulation of NKG2D, thereby preventing NK cell‑mediated cytotoxicity toward renal proximal tubular epithelial cells in renal IRI, which in turn improves the survival of these cells.
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Copy and paste a formatted citation
Spandidos Publications style
Song H, Kim Y, Park G, Kim Y, Kim S, Lee H, Chung WY, Park SJ, Han S, Cho D, Cho D, et al: Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. Int J Mol Med 36: 1180-1188, 2015.
APA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H. ... Hur, D. (2015). Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. International Journal of Molecular Medicine, 36, 1180-1188. https://doi.org/10.3892/ijmm.2015.2317
MLA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36.4 (2015): 1180-1188.
Chicago
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36, no. 4 (2015): 1180-1188. https://doi.org/10.3892/ijmm.2015.2317
Copy and paste a formatted citation
x
Spandidos Publications style
Song H, Kim Y, Park G, Kim Y, Kim S, Lee H, Chung WY, Park SJ, Han S, Cho D, Cho D, et al: Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. Int J Mol Med 36: 1180-1188, 2015.
APA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H. ... Hur, D. (2015). Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. International Journal of Molecular Medicine, 36, 1180-1188. https://doi.org/10.3892/ijmm.2015.2317
MLA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36.4 (2015): 1180-1188.
Chicago
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36, no. 4 (2015): 1180-1188. https://doi.org/10.3892/ijmm.2015.2317
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