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International Journal of Molecular Medicine
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Article

Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways

  • Authors:
    • Qinghua Yao
    • Miao Lin
    • Yuqi Wang
    • Yuebiao Lai
    • Jingjing Hu
    • Ting Fu
    • Lu Wang
    • Shuyuan Lin
    • Liangliang Chen
    • Yong Guo
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Traditional Chinese Medicine Oncology, Zhejiang Cancer Hospital, Banshan Qiao, Hangzhou, Zhejiang 310022, P.R. China, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China
  • Pages: 1118-1126
    |
    Published online on: August 26, 2015
       https://doi.org/10.3892/ijmm.2015.2327
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Abstract

Curcumin has been found to exhibit anticancer activity and certain studies have shown that curcumin triggers the apoptosis of human A549 lung adenocarcinoma cells. However, the mechanism underlying curcumin‑mediated apoptosis is not completely understood. The present study was designed to investigate the effect of curcumin on the induction of apoptosis and apoptosis‑related factors in human A549 lung adenocarcinoma cells. Treatment of A549 cells with curcumin caused a concentration‑dependent inhibition of cell growth and an increase in apoptosis, as confirmed by THE MTT assay, flow cytometry and morphology analysis. Curcumin‑treatment of A549 cells induced a loss of the mitochondrial membrane potential and increased cytosolic cytochrome c. Furthermore, curcumin‑induced apoptosis was accompanied by changes in intracellular oxidative stress‑related enzymes, including decreased intracellular reactive oxygen species levels, increased superoxide dismutase and decreased malondialdehyde and 4‑hydroxynonenal. In addition, induction of apoptosis was also accompanied by phosphorylation and activation of mitogen‑activated protein kinase signaling pathway factors c‑Jun N‑terminal kinase, p38 and extracellular signal-regulated kinase.
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Copy and paste a formatted citation
Spandidos Publications style
Yao Q, Lin M, Wang Y, Lai Y, Hu J, Fu T, Wang L, Lin S, Chen L, Guo Y, Guo Y, et al: Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways. Int J Mol Med 36: 1118-1126, 2015.
APA
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T. ... Guo, Y. (2015). Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways. International Journal of Molecular Medicine, 36, 1118-1126. https://doi.org/10.3892/ijmm.2015.2327
MLA
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T., Wang, L., Lin, S., Chen, L., Guo, Y."Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways". International Journal of Molecular Medicine 36.4 (2015): 1118-1126.
Chicago
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T., Wang, L., Lin, S., Chen, L., Guo, Y."Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways". International Journal of Molecular Medicine 36, no. 4 (2015): 1118-1126. https://doi.org/10.3892/ijmm.2015.2327
Copy and paste a formatted citation
x
Spandidos Publications style
Yao Q, Lin M, Wang Y, Lai Y, Hu J, Fu T, Wang L, Lin S, Chen L, Guo Y, Guo Y, et al: Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways. Int J Mol Med 36: 1118-1126, 2015.
APA
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T. ... Guo, Y. (2015). Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways. International Journal of Molecular Medicine, 36, 1118-1126. https://doi.org/10.3892/ijmm.2015.2327
MLA
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T., Wang, L., Lin, S., Chen, L., Guo, Y."Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways". International Journal of Molecular Medicine 36.4 (2015): 1118-1126.
Chicago
Yao, Q., Lin, M., Wang, Y., Lai, Y., Hu, J., Fu, T., Wang, L., Lin, S., Chen, L., Guo, Y."Curcumin induces the apoptosis of A549 cells via oxidative stress and MAPK signaling pathways". International Journal of Molecular Medicine 36, no. 4 (2015): 1118-1126. https://doi.org/10.3892/ijmm.2015.2327
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