Open Access

Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy

  • Authors:
    • Dongdong Chen
    • Xiaoyang Tao
    • Yang Wang
    • Fengxuan Tian
    • Yongxin Wei
    • Guilin Chen
    • Haitao Shen
    • Zhong Wang
    • Zhengquan Yu
    • Haiying Li
    • Gang Chen
  • View Affiliations

  • Published online on: October 9, 2015     https://doi.org/10.3892/ijmm.2015.2365
  • Pages: 1563-1571
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Delayed reendothelialization and intimal hyper­plasia (IH) contribute to the failure of vascular interventions. Curcumin (Cur) has been used for various types of diseases with antioxidant, antiproliferative and anti‑inflammatory effects. However, investigations involving the application of Cur in inhibiting IH are limited. The aim of the present study was to evaluate the potential therapeutic effects of Cur and its underlying mechanisms on a rat model of carotid artery (CA) intimal injury. In vitro, an endothelial cell (EC) migration assay was conducted using cultured primary human umbilical vein endothelial cells (HUVECs) that were exposed to Cur. In vivo, CA angioplasty injury was used to generate a rat model of intimal injury. CAs were collected at 3 days, and 1 and 4 weeks after injury, respectively, for western blot analysis and double-immunofluorescence analyses, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling staining, oxidative stress indicator analysis and hematoxylin and eosin staining of the neointima. In vivo, Cur significantly enhanced the migration and healing of HUVECs and simultaneously promoted microtubule-associated protein light chain 3-II (LC3-II) expression when HUVECs were subjected to an artificial scratch. In vitro, endangium from the Cur-treated rats exhibited a significantly reduced number of apoptotic ECs and oxidative stress level compared to that of the sham group. In addition, Cur treatment markedly improved quantification of the LC3-II concomitant with the downregulation of p62 in the injured CA. At 1 week following injury, sizable neointimal lesions had developed, although prominent intima thickening was not observed. At 4 weeks, apparent hemadostenosis occurred resulting from the exorbitance IH. Cur treatment markedly reduced the thickness of the neointimal lesion. It is noteworthy that high-dose Cur may have exerted more significant effects than low-dose Cur. Cur can potentially become a therapeutic drug for angiostenosis by imparting a protective effect that accelerates reendothelialization and ameliorates IH and was mediated by its pro-autophagic effect.
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December-2015
Volume 36 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Chen D, Tao X, Wang Y, Tian F, Wei Y, Chen G, Shen H, Wang Z, Yu Z, Li H, Li H, et al: Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy. Int J Mol Med 36: 1563-1571, 2015
APA
Chen, D., Tao, X., Wang, Y., Tian, F., Wei, Y., Chen, G. ... Chen, G. (2015). Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy. International Journal of Molecular Medicine, 36, 1563-1571. https://doi.org/10.3892/ijmm.2015.2365
MLA
Chen, D., Tao, X., Wang, Y., Tian, F., Wei, Y., Chen, G., Shen, H., Wang, Z., Yu, Z., Li, H., Chen, G."Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy". International Journal of Molecular Medicine 36.6 (2015): 1563-1571.
Chicago
Chen, D., Tao, X., Wang, Y., Tian, F., Wei, Y., Chen, G., Shen, H., Wang, Z., Yu, Z., Li, H., Chen, G."Curcumin accelerates reendothelialization and ameliorates intimal hyperplasia in balloon-injured rat carotid artery via the upregulation of endothelial cell autophagy". International Journal of Molecular Medicine 36, no. 6 (2015): 1563-1571. https://doi.org/10.3892/ijmm.2015.2365