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Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress

  • Authors:
    • Pingdong Lin
    • Xiaping Weng
    • Fayuan Liu
    • Yuhuan Ma
    • Houhuang Chen
    • Xiang Shao
    • Wenwei Zheng
    • Xianxiang Liu
    • Hongzhi Ye
    • Xihai Li
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China
    Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1519-1528
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    Published online on: October 22, 2015
       https://doi.org/10.3892/ijmm.2015.2387
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Abstract

Chondrocyte apoptosis triggered by endoplasmic reticulum (ER) stress plays a vital role in the pathogenesis of osteoarthritis (OA). Bushen Zhuangjin decoction (BZD) has been widely used in the treatment of OA. However, the cellular and molecular mechanisms responsible for the inhibitory effects of BZD on chondrocyte apoptosis remain to be elucidated. In the present study, we investigated the effects of BZD on ER stress‑induced chondrocyte apoptosis using a chondrocyte in vitro model of OA. Chondrocytes obtained from the articular cartilage of the knee joints of Sprague Dawley (SD) rats were detected by immunohistochemical staining for type Ⅱ collagen. The ER stress‑mediated apoptosis of tunicamycin (TM)‑stimulated chondrocytes was detected using 4‑phenylbutyric acid (4‑PBA). We found that 4‑PBA inhibited TM‑induced chondrocyte apoptosis, which confirmed the successful induction of chondrocyte apoptosis. BZD enhanced the viability of the TM‑stimulated chondrocytes in a dose‑ and time‑dependent manner, as shown by MTT assay. The apoptotic rate and the loss of mitochondrial membrane potential (ΔΨm) of the TM‑stimulated chondrocytes treated with BZD was markedly decreased compared with those of chondrocytes not treated with BZD, as shown by 4',6-diamidino-2-phenylindole (DAPI) staining, Annexin V-FITC binding assay and JC-1 assay. To further elucidate the mechanisms responsible for the inhibitory effects of BZD on TM‑induced chondrocyte apoptosis mediated by ER stress, the mRNA and protein expression levels of binding immunoglobulin protein (Bip), X‑box binding protein 1 (Xbp1), activating transcription factor 4 (Atf4), C/EBP‑homologous protein (Chop), caspase‑9, caspase‑3, B‑cell lymphoma 2 (Bcl‑2) and Bcl-2‑associated X protein (Bax) were measured by reverse transcription‑polymerase chain reaction (RT‑PCR) and western blot analysis. In the TM‑stimulated chondrocytes treated with BZD, the mRNA and protein expression levels of Bip, Atf4, Chop, caspase‑9, caspase‑3 and Bax were significantly decreased, whereas the mRNA and protein expression levels of Xbp1 and Bcl‑2 were significantly increased compared with the TM‑stimulated chondrocytes not treated with BZD. Additionally, all our findings demonstrated that there was no significant difference between the TM‑stimulated chondrocytes treated with BZD and those treated with 4‑PBA. Taken together, our results indicate that BZD inhibits TM‑induced chondrocyte apoptosis mediated by ER stress. Thus, BZD may be a potential therapeutic agent for use in the treatment of OA.
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Copy and paste a formatted citation
Spandidos Publications style
Lin P, Weng X, Liu F, Ma Y, Chen H, Shao X, Zheng W, Liu X, Ye H, Li X, Li X, et al: Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress. Int J Mol Med 36: 1519-1528, 2015.
APA
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X. ... Li, X. (2015). Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress. International Journal of Molecular Medicine, 36, 1519-1528. https://doi.org/10.3892/ijmm.2015.2387
MLA
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X., Zheng, W., Liu, X., Ye, H., Li, X."Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress". International Journal of Molecular Medicine 36.6 (2015): 1519-1528.
Chicago
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X., Zheng, W., Liu, X., Ye, H., Li, X."Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress". International Journal of Molecular Medicine 36, no. 6 (2015): 1519-1528. https://doi.org/10.3892/ijmm.2015.2387
Copy and paste a formatted citation
x
Spandidos Publications style
Lin P, Weng X, Liu F, Ma Y, Chen H, Shao X, Zheng W, Liu X, Ye H, Li X, Li X, et al: Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress. Int J Mol Med 36: 1519-1528, 2015.
APA
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X. ... Li, X. (2015). Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress. International Journal of Molecular Medicine, 36, 1519-1528. https://doi.org/10.3892/ijmm.2015.2387
MLA
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X., Zheng, W., Liu, X., Ye, H., Li, X."Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress". International Journal of Molecular Medicine 36.6 (2015): 1519-1528.
Chicago
Lin, P., Weng, X., Liu, F., Ma, Y., Chen, H., Shao, X., Zheng, W., Liu, X., Ye, H., Li, X."Bushen Zhuangjin decoction inhibits TM-induced chondrocyte apoptosis mediated by endoplasmic reticulum stress". International Journal of Molecular Medicine 36, no. 6 (2015): 1519-1528. https://doi.org/10.3892/ijmm.2015.2387
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