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Article

Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer

  • Authors:
    • Weigang Guo
    • Yongxing Zhang
    • Yi Zhang
    • Yu Shi
    • Junjie Xi
    • Hong Fan
    • Songtao Xu
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Zhongshan Hospital of Fudan University, Shanghai 200032, P.R. China
  • Pages: 1720-1726
    |
    Published online on: October 22, 2015
       https://doi.org/10.3892/ijmm.2015.2388
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Abstract

In order to identify novel non-invasive biomarkers with high accuracy for the screening of non-small cell lung cancer (NSCLC), we investigated the predictive power of 4 microRNAs (miR-146, miR-204, miR-106a and miR-124) in plasma samples obtained from patients with NSCLC and healthy controls (n=50; training phase) by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). We found that the levels of miR-204 in the patients with NSCLC were significantly dysregulated compared with the healthy controls, and thus this miRNA was selected for further validation. For the validation phase, RT-qPCR was performed on plasma samples from patients with NSCLC and healthy controls (n=176) in order to examine the expression levels of the candidate miRNA, miR-204. The results revealed that the plasma levels of miR-204 were significantly downregulated in the patients with NSCLC compared with the healthy controls (p<0.001). The value of the area under the receiver operating characteristic (ROC) curve obtained for miR-204 was 0.809 (sensitivity, 76%; specificity, 82%), which was higher than the values obtained for carcinoembryonic antigen (CEA) and carbohydrate antigen 19-9 (CA19-9). The expression of miR-204 in plasma significantly correlated with the tumor stage (p=0.009) and distant metastasis (p=0.048). A log-rank test revealed that lower plasma levels of miR-204 were associated with a shorter overall survival and disease-free survival (p=0.006 and 0.0065, respectively). Both univariate and multivariate Cox regression analyses indicated that a lower miR-204 expression level in plasma was a prognostic factor with a relative risk of death of 1.936 and 1.712, respectively. On the whole, our results suggest that the decreased expression of miR-204 in plasma is a promising biomarker for the detection of NSCLC and the prediction of poor survival in patients with the disease.
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Copy and paste a formatted citation
Spandidos Publications style
Guo W, Zhang Y, Zhang Y, Shi Y, Xi J, Fan H and Xu S: Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer. Int J Mol Med 36: 1720-1726, 2015.
APA
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., & Xu, S. (2015). Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer. International Journal of Molecular Medicine, 36, 1720-1726. https://doi.org/10.3892/ijmm.2015.2388
MLA
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., Xu, S."Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer". International Journal of Molecular Medicine 36.6 (2015): 1720-1726.
Chicago
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., Xu, S."Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer". International Journal of Molecular Medicine 36, no. 6 (2015): 1720-1726. https://doi.org/10.3892/ijmm.2015.2388
Copy and paste a formatted citation
x
Spandidos Publications style
Guo W, Zhang Y, Zhang Y, Shi Y, Xi J, Fan H and Xu S: Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer. Int J Mol Med 36: 1720-1726, 2015.
APA
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., & Xu, S. (2015). Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer. International Journal of Molecular Medicine, 36, 1720-1726. https://doi.org/10.3892/ijmm.2015.2388
MLA
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., Xu, S."Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer". International Journal of Molecular Medicine 36.6 (2015): 1720-1726.
Chicago
Guo, W., Zhang, Y., Zhang, Y., Shi, Y., Xi, J., Fan, H., Xu, S."Decreased expression of miR-204 in plasma is associated with a poor prognosis in patients with non-small cell lung cancer". International Journal of Molecular Medicine 36, no. 6 (2015): 1720-1726. https://doi.org/10.3892/ijmm.2015.2388
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