Open Access

Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway

  • Authors:
    • Jiajia Yan
    • Jialin Duan
    • Xiaoxiao Wu
    • Chao Guo
    • Ying Yin
    • Yanrong Zhu
    • Tianxin Hu
    • Guo Wei
    • Aidong Wen
    • Miaomiao Xi
  • View Affiliations

  • Published online on: October 23, 2015     https://doi.org/10.3892/ijmm.2015.2391
  • Pages: 1538-1546
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

It was previously shown that total saponins extracted from Aralia taibaiensis (sAT) have potent antioxidant activities for treating diabetes mellitus and attenuate d-galactose-induced aging. Since diabetes mellitus and aging are closely associated with cardiac dysfunction, particularly ischemic heart disease, sAT may have potential protective activity against myocardial ischemia/reperfusion injury (MI/RI). However, the anti-MI/RI effects of sAT have yet to be examined, and the possible molecular mechanisms remain to be determined. The present study was undertaken to investigate the anti-MI/RI activities of sAT and to elucidate the mechanisms underlying these effects in rats using TUNEL and Hoechst 33258 staining. The results confirmed the cardioprotective effects in vivo and elucidated the potential molecular mechanisms of sAT in vitro. Pretreatment with sAT significantly reduced infarct size, decreased the levels of lactate dehydrogenase and creatine kinase in the serum and blocked apoptosis. In addition, sAT inhibited A/R-induced apoptosis by decreasing DNA strand breaks, caspase-3 activity and cytochrome c release in H9c2 cells. Furthermore, sAT markedly increased the phosphorylation of AMP-activated protein kinase (AMPK) and acetyl CoA carboxylase and elevated the Bcl2/Bcl-2-associated X protein ratio. These effects were blocked by compound C. The results suggested that sAT pretreatment exerts protective effects on myocardial cells in vitro and in vivo against MI/RI-induced apoptosis by activating AMPK pathway.
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December-2015
Volume 36 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yan J, Duan J, Wu X, Guo C, Yin Y, Zhu Y, Hu T, Wei G, Wen A, Xi M, Xi M, et al: Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway. Int J Mol Med 36: 1538-1546, 2015
APA
Yan, J., Duan, J., Wu, X., Guo, C., Yin, Y., Zhu, Y. ... Xi, M. (2015). Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway. International Journal of Molecular Medicine, 36, 1538-1546. https://doi.org/10.3892/ijmm.2015.2391
MLA
Yan, J., Duan, J., Wu, X., Guo, C., Yin, Y., Zhu, Y., Hu, T., Wei, G., Wen, A., Xi, M."Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway". International Journal of Molecular Medicine 36.6 (2015): 1538-1546.
Chicago
Yan, J., Duan, J., Wu, X., Guo, C., Yin, Y., Zhu, Y., Hu, T., Wei, G., Wen, A., Xi, M."Total saponins from Aralia taibaiensis protect against myocardial ischemia/reperfusion injury through AMPK pathway". International Journal of Molecular Medicine 36, no. 6 (2015): 1538-1546. https://doi.org/10.3892/ijmm.2015.2391