Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
January-2016 Volume 37 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2016 Volume 37 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples

  • Authors:
    • Xin Wang
    • Xiang Li
    • Ying-Nan Zeng
    • Fa He
    • Xiao-Min Yang
    • Feng Guan
  • View Affiliations / Copyright

    Affiliations: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China , Wuxi Medical School, Jiangnan University, Wuxi, Jiangsu 214122, P.R. China, Department of Surgical Oncology, The First Affiliated Hospital, Medicine School, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
  • Pages: 197-206
    |
    Published online on: October 27, 2015
       https://doi.org/10.3892/ijmm.2015.2395
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Polysialic acid (PSA) is highly expressed during embryonic development, but barely expressed during postnatal development, and may be ‘re-expressed’ in cancer tissues. In this study, motility and migration assays were performed to compare the changes in cell behavior between non-malignant and maligant cells. Next, the expression levels of PSA were evaluated in 4 human and mouse normal breast or breast cancer (BC) cell lines using 1,2-diamino-4,5-methylenedioxybenzene-labeling HPLC technology, as well as in human clinical BC tissue samples. PSA expression was significantly higher in malignant cells (where it appeared to facilitate cell migration and motility) than in non-malignant cells. Enhanced PSA expression levels were also observed during epithelial-mesenchymal transition (EMT), a leading cause of cancer cell metastasis, which was induced in the NMuMG and MCF10A cells by treatment with transforming growth factor-β1 (TGF-β1). An increased PSA expression also correlated with the disease stage in the patients with BC (P<0.0001). Using RT-qPCR, we found that polysialyltransferase ST8SiaIV (PST) and polysialyltransferase ST8SiaII (STX), which are responsible for PSA synthesis, were differently expressed in the tested BC samples. However, PST, but not STX, was re-expressed in 14 out of 20 clinical BC samples. The findings of the present study indicate that the pathophysiology of BC involves the aberrant regulation of PSA expression and PST gene expression.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Schnaar RL, Gerardy-Schahn R and Hildebrandt H: Sialic acids in the brain: gangliosides and polysialic acid in nervous system development, stability, disease, and regeneration. Physiol Rev. 94:461–518. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Cheray M, Petit D, Forestier L, Karayan-Tapon L, Maftah A, Jauberteau MO, Battu S, Gallet FP and Lalloué F: Glycosylation-related gene expression is linked to differentiation status in glioblastomas undifferentiated cells. Cancer Lett. 312:24–32. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Hildebrandt H, Mühlenhoff M and Gerardy-Schahn R: Polysialylation of NCAM. Adv Exp Med Biol. 663:95–109. 2010. View Article : Google Scholar

4 

Scheidegger EP, Lackie PM, Papay J and Roth J: In vitro and in vivo growth of clonal sublines of human small cell lung carcinoma is modulated by polysialic acid of the neural cell adhesion molecule. Lab Invest. 70:95–106. 1994.PubMed/NCBI

5 

Kameda K, Shimada H, Ishikawa T, Takimoto A, Momiyama N, Hasegawa S, Misuta K, Nakano A, Nagashima Y and Ichikawa Y: Expression of highly polysialylated neural cell adhesion molecule in pancreatic cancer neural invasive lesion. Cancer Lett. 137:201–207. 1999. View Article : Google Scholar : PubMed/NCBI

6 

Suzuki M, Suzuki M, Nakayama J, Suzuki A, Angata K, Chen S, Sakai K, Hagihara K, Yamaguchi Y and Fukuda M: Polysialic acid facilitates tumor invasion by glioma cells. Glycobiology. 15:887–894. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Korja M, Jokilammi A, Salmi TT, Kalimo H, Pelliniemi TT, Isola J, Rantala I, Haapasalo H and Finne J: Absence of polysialylated NCAM is an unfavorable prognostic phenotype for advanced stage neuroblastoma. BMC Cancer. 9:572009. View Article : Google Scholar : PubMed/NCBI

8 

Eckhardt M, Mühlenhoff M, Bethe A, Koopman J, Frosch M and Gerardy-Schahn R: Molecular characterization of eukaryotic polysialyltransferase-1. Nature. 373:715–718. 1995. View Article : Google Scholar : PubMed/NCBI

9 

Schreiber SC, Giehl K, Kastilan C, Hasel C, Mühlenhoff M, Adler G, Wedlich D and Menke A: Polysialylated NCAM represses E-cadherin-mediated cell-cell adhesion in pancreatic tumor cells. Gastroenterology. 134:1555–1566. 2008. View Article : Google Scholar : PubMed/NCBI

10 

Tanaka F, Otake Y, Nakagawa T, Kawano Y, Miyahara R, Li M, Yanagihara K, Nakayama J, Fujimoto I, Ikenaka K and Wada H: Expression of polysialic acid and STX, a human polysialyltransferase, is correlated with tumor progression in non-small cell lung cancer. Cancer Res. 60:3072–3080. 2000.PubMed/NCBI

11 

Raval GN, Parekh LJ, Patel DD, Jha FP, Sainger RN and Patel PS: Clinical usefulness of alterations in sialic acid, sialyl transferase and sialoproteins in breast cancer. Indian J Clin Biochem. 19:60–71. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

13 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Thiery JP: Epithelial-mesenchymal transitions in tumour progression. Nat Rev Cancer. 2:442–454. 2002. View Article : Google Scholar : PubMed/NCBI

15 

Varki A: Sialic acids in human health and disease. Trends Mol Med. 14:351–360. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Liu T, Guo Z, Yang Q, Sad S and Jennings HJ: Biochemical engineering of surface alpha 2-8 polysialic acid for immunotargeting tumor cells. J Biol Chem. 275:32832–32836. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Yang X, Pursell B, Lu S, Chang T-K and Mercurio AM: Regulation of β4-integrin expression by epigenetic modifications in the mammary gland and during the epithelial-to-mesenchymal transition. J Cell Sci. 122:2473–2480. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Yang G, Cui T, Wang Y, Sun S, Ma T, Wang T, Chen Q and Li Z: Selective isolation and analysis of glycoprotein fractions and their glycomes from hepatocellular carcinoma sera. Proteomics. 13:1481–1498. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Tan Z, Lu W, Li X, Yang G, Guo J, Yu H, Li Z and Guan F: Altered N-Glycan expression profile in epithelial-to-mesenchymal transition of NMuMG cells revealed by an integrated strategy using mass spectrometry and glycogene and lectin microarray analysis. J Proteome Res. 13:2783–2795. 2014. View Article : Google Scholar : PubMed/NCBI

20 

Varki A and Diaz S: The release and purification of sialic acids from glycoconjugates: methods to minimize the loss and migration of O-acetyl groups. Anal Biochem. 137:236–247. 1984. View Article : Google Scholar : PubMed/NCBI

21 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−ΔΔC(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

22 

Albrecht-Buehler G: The phagokinetic tracks of 3T3 cells. Cell. 11:395–404. 1977. View Article : Google Scholar : PubMed/NCBI

23 

Guan F, Handa K and Hakomori SI: Specific glycosphingolipids mediate epithelial-to-mesenchymal transition of human and mouse epithelial cell lines. Proc Natl Acad Sci USA. 106:7461–7466. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Grosheva I, Shtutman M, Elbaum M and Bershadsky AD: p120 catenin affects cell motility via modulation of activity of Rho-family GTPases: A link between cell-cell contact formation and regulation of cell locomotion. J Cell Sci. 114:695–707. 2001.PubMed/NCBI

25 

Hosokawa N, Wada I, Hasegawa K, Yorihuzi T, Tremblay LO, Herscovics A and Nagata K: A novel ER α-mannosidase-like protein accelerates ER-associated degradation. EMBO Rep. 2:415–422. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Watanabe S, Kokuho T, Takahashi H, Takahashi M, Kubota T and Inumaru S: Sialylation of N-glycans on the recombinant proteins expressed by a baculovirus-insect cell system under β-N-acetylglucosaminidase inhibition. J Biol Chem. 277:5090–5093. 2002. View Article : Google Scholar

27 

Barranco WT and Eckhert CD: Cellular changes in boric acid-treated DU-145 prostate cancer cells. Br J Cancer. 94:884–890. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Adorno M, Cordenonsi M, Montagner M, Dupont S, Wong C, Hann B, Solari A, Bobisse S, Rondina MB, Guzzardo V, et al: A Mutant-p53/Smad complex opposes p63 to empower TGFbeta-induced metastasis. Cell. 137:87–98. 2009. View Article : Google Scholar : PubMed/NCBI

29 

Kelishadi R, Hashemi Pour M, Famouri F, Sabet B and Sanei M: The impact of breast-feeding in prevention of obesity in children. J Qazvin Univ Med Sci. 9:88–93. 2005.

30 

Gunasinghe NP, Wells A, Thompson EW and Hugo HJ: Mesenchymal-epithelial transition (MET) as a mechanism for metastatic colonisation in breast cancer. Cancer Metastasis Rev. 31:469–478. 2012. View Article : Google Scholar : PubMed/NCBI

31 

Christofori G: New signals from the invasive front. Nature. 441:444–450. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Gupta GP and Massagué J: Cancer metastasis: building a framework. Cell. 127:679–695. 2006. View Article : Google Scholar : PubMed/NCBI

33 

Dall'Olio F and Chiricolo M: Sialyltransferases in cancer. Glycoconj J. 18:841–850. 2001. View Article : Google Scholar

34 

Gotzmann J, Mikula M, Eger A, Schulte-Hermann R, Foisner R, Beug H and Mikulits W: Molecular aspects of epithelial cell plasticity: implications for local tumor invasion and metastasis. Mutat Res. 566:9–20. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Falconer RA, Errington RJ, Shnyder SD, Smith PJ and Patterson LH: Polysialyltransferase: a new target in metastatic cancer. Curr Cancer Drug Targets. 12:925–939. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Lin S, Kemmner W, Grigull S and Schlag PM: Cell surface alpha 2,6 sialylation affects adhesion of breast carcinoma cells. Exp Cell Res. 276:101–110. 2002. View Article : Google Scholar : PubMed/NCBI

37 

Tanaka F, Otake Y, Nakagawa T, Kawano Y, Miyahara R, Li M, Yanagihara K, Inui K, Oyanagi H, Yamada T, et al: Prognostic significance of polysialic acid expression in resected non-small cell lung cancer. Cancer Res. 61:1666–1670. 2001.PubMed/NCBI

38 

Angata K, Suzuki M and Fukuda M: ST8Sia II and ST8Sia IV polysialyltransferases exhibit marked differences in utilizing various acceptors containing oligosialic acid and short polysialic acid. The basis for cooperative polysialylation by two enzymes. J Biol Chem. 277:36808–36817. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang X, Li X, Zeng Y, He F, Yang X and Guan F: Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples. Int J Mol Med 37: 197-206, 2016.
APA
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., & Guan, F. (2016). Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples. International Journal of Molecular Medicine, 37, 197-206. https://doi.org/10.3892/ijmm.2015.2395
MLA
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., Guan, F."Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples". International Journal of Molecular Medicine 37.1 (2016): 197-206.
Chicago
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., Guan, F."Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples". International Journal of Molecular Medicine 37, no. 1 (2016): 197-206. https://doi.org/10.3892/ijmm.2015.2395
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Li X, Zeng Y, He F, Yang X and Guan F: Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples. Int J Mol Med 37: 197-206, 2016.
APA
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., & Guan, F. (2016). Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples. International Journal of Molecular Medicine, 37, 197-206. https://doi.org/10.3892/ijmm.2015.2395
MLA
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., Guan, F."Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples". International Journal of Molecular Medicine 37.1 (2016): 197-206.
Chicago
Wang, X., Li, X., Zeng, Y., He, F., Yang, X., Guan, F."Enhanced expression of polysialic acid correlates with malignant phenotype in breast cancer cell lines and clinical tissue samples". International Journal of Molecular Medicine 37, no. 1 (2016): 197-206. https://doi.org/10.3892/ijmm.2015.2395
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team