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MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway

  • Authors:
    • Lihua Wang
    • Guixian Song
    • Ming Liu
    • Bin Chen
    • Yumei Chen
    • Yahui Shen
    • Jingai Zhu
    • Xiaoyu Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Neonatology, Nanjing Children's Hospital, Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Cardiology, Taizhou People's Hospital, Taizhou, Jiangsu 225300, P.R. China, Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Children Health Care, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 47-55
    |
    Published online on: November 2, 2015
       https://doi.org/10.3892/ijmm.2015.2399
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Abstract

Our previous study reported that microRNA-375 (miR-375) is significantly upregulated in ventricular septal myocardial tissues from 22‑week‑old fetuses with ventricular septal defect as compared with normal controls. In the present study, the specific effects of miR‑375 on P19 cell differentiation into cardiomyocyte‑like cells were investigated. Stable P19 cell lines overexpressing miR‑375 or containing empty vector were established, which could be efficiently induced into cardiomyocyte‑like cells in the presence of dimethyl sulfoxide in vitro. miR‑375 overexpression was verified using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Cell proliferation was determined according to total cell counts; cell cycle distribution and apoptosis levels were examined using flow cytometry. Apoptosis‑related morphological changes were observed using Hoechst staining and fluorescence microscopy. During P19 cell differentiation, the cardiomyogenesis‑related mRNAs (cardiac troponin T, GATA binding protein 4, myocyte‑specific enhancer factor 2C) and mRNAs involved in the Notch signaling pathway (Notch2, Delta‑like 1 and hes family bHLH transcription factor 1) were detected at days 0, 4, 6 and 10. Their differential expression was examined using RT‑qPCR; the apoptosis‑related genes BAX and Bcl‑2 were also detected using this method. The corresponding proteins were evaluated by western blotting. Compared with the control group, miR‑375 overexpression inhibited proliferation but promoted apoptosis in P19 cells, and the associated mRNAs and proteins were decreased during differentiation. miR‑375 has an important role in cardiomyocyte differentiation, and can disrupt this process via the Notch signaling pathway. The present findings contribute to the understanding of the mechanisms of congenital heart disease and facilitate the development of new gene therapies.
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View References

1 

Hoffman JI and Kaplan S: The incidence of congenital heart disease. J Am Coll Cardiol. 39:1890–1900. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Srivastava D: Making or breaking the heart: From lineage determination to morphogenesis. Cell. 126:1037–1048. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Somerville J: Grown-up congenital heart disease - medical demands look back, look forward 2000. Thorac Cardiovasc Surg. 49:21–26. 2001. View Article : Google Scholar : PubMed/NCBI

4 

van der Linde D, Konings EE, Slager MA, Witsenburg M, Helbing WA, Takkenberg JJ and Roos-Hesselink JW: Birth prevalence of congenital heart disease worldwide: A systematic review and meta-analysis. J Am Coll Cardiol. 58:2241–2247. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Garside VC, Chang AC, Karsan A and Hoodless PA: Co-ordinating Notch, BMP, and TGF-β signaling during heart valve development. Cell Mol Life Sci. 70:2899–2917. 2013. View Article : Google Scholar

6 

High FA and Epstein JA: The multifaceted role of Notch in cardiac development and disease. Nat Rev Genet. 9:49–61. 2008. View Article : Google Scholar

7 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Yates LA, Norbury CJ and Gilbert RJ: The long and short of microRNA. Cell. 153:516–519. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Gangaraju VK and Lin H: MicroRNAs: Key regulators of stem cells. Nat Rev Mol Cell Biol. 10:116–125. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Ambros V: The functions of animal microRNAs. Nature. 431:350–355. 2004. View Article : Google Scholar : PubMed/NCBI

11 

Feng Y and Yu X: Cardinal roles of miRNA in cardiac development and disease. Sci China Life Sci. 54:1113–1120. 2011. View Article : Google Scholar

12 

Thum T, Catalucci D and Bauersachs J: MicroRNAs: Novel regulators in cardiac development and disease. Cardiovasc Res. 79:562–570. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Kasinski AL and Slack FJ: Epigenetics and genetics MicroRNAs en route to the clinic: Progress in validating and targeting microRNAs for cancer therapy. Nat Rev Cancer. 11:849–864. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Callegari E, Gramantieri L, Domenicali M, D'Abundo L, Sabbioni S and Negrini M: MicroRNAs in liver cancer: A model for investigating pathogenesis and novel therapeutic approaches. Cell Death Differ. 22:46–57. 2015. View Article : Google Scholar

15 

Zhu S, Cao L, Zhu J, Kong L, Jin J, Qian L, Zhu C, Hu X, Li M and Guo X: et al Identification of maternal serum microRNAs as novel non-invasive biomarkers for prenatal detection of fetal congenital heart defects. Clin Chim Acta. 424:66–72. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Yu Z, Han S, Hu P, Zhu C, Wang X, Qian L and Guo X: Potential role of maternal serum microRNAs as a biomarker for fetal congenital heart defects. Med Hypotheses. 76:424–426. 2011. View Article : Google Scholar

17 

Andersson ER, Sandberg R and Lendahl U: Notch signaling: Simplicity in design, versatility in function. Development. 138:3593–3612. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Kopan R and Ilagan MXG: The canonical Notch signaling pathway: Unfolding the activation mechanism. Cell. 137:216–233. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Iso T, Kedes L and Hamamori Y: HES and HERP families: Multiple effectors of the Notch signaling pathway. J Cell Physiol. 194:237–255. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Chang ACY, Fu Y, Garside VC, Niessen K, Chang L, Fuller M, Setiadi A, Smrz J, Kyle A and Minchinton A: et al Notch initiates the endothelial-to-mesenchymal transition in the atrioventricular canal through autocrine activation of soluble guanylyl cyclase. Dev Cell. 21:288–300. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Chau MDL, Tuft R, Fogarty K and Bao ZZ: Notch signaling plays a key role in cardiac cell differentiation. Mech Dev. 123:626–640. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Bishayee K, Ghosh S, Mukherjee A, Sadhukhan R, Mondal J and Khuda-Bukhsh AR: Quercetin induces cytochrome-c release and ROS accumulation to promote apoptosis and arrest the cell cycle in G2/M, in cervical carcinoma: Signal cascade and drug-DNA interaction. Cell Prolif. 46:153–163. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Verheugt CL, Uiterwaal CS, Grobbee DE and Mulder BJ: Long-term prognosis of congenital heart defects: A systematic review. Int J Cardiol. 131:25–32. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Zomer AC, Vaartjes I, van der Velde ET, de Jong HM, Konings TC, Wagenaar LJ, Heesen WF, Eerens F, Baur LH and Grobbee DE: et al Heart failure admissions in adults with congenital heart disease; risk factors and prognosis. Int J Cardiol. 168:2487–2493. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Sayed AS, Xia K, Salma U, Yang T and Peng J: Diagnosis, prognosis and therapeutic role of circulating miRNAs in cardiovascular diseases. Heart Lung Circ. 23:503–510. 2014. View Article : Google Scholar : PubMed/NCBI

26 

El Ouaamari A, Baroukh N, Martens GA, Lebrun P, Pipeleers D and van Obberghen E: miR-375 targets 3′-phosphoinositide-dependent protein kinase-1 and regulates glucose-induced biological responses in pancreatic beta-cells. Diabetes. 57:2708–2717. 2008. View Article : Google Scholar : PubMed/NCBI

27 

Kloosterman WP, Lagendijk AK, Ketting RF, Moulton JD and Plasterk RH: Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biol. 5:e2032007. View Article : Google Scholar : PubMed/NCBI

28 

Zhang H, Mishra A, Chintagari NR, Gou D and Liu L: Micro-RNA-375 inhibits lung surfactant secretion by altering cytoskeleton reorganization. IUBMB Life. 62:78–83. 2010.

29 

Cao L, Kong LP, Yu ZB, Han SP, Bai YF, Zhu J, Hu X, Zhu C, Zhu S and Guo XR: microRNA expression profiling of the developing mouse heart. Int J Mol Med. 30:1095–1104. 2012.PubMed/NCBI

30 

Adams JM and Cory S: The Bcl-2 protein family: Arbiters of cell survival. Science. 281:1322–1326. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Golestani Eimani B, Sanati MH, Houshmand M, Ataei M, Akbarian F and Shakhssalim N: Expression and prognostic significance of bcl-2 and bax in the progression and clinical outcome of transitional bladder cell carcinoma. Cell J. 15:356–363. 2014.PubMed/NCBI

32 

Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI

33 

van der Heyden MA and Defize LH: Twenty one years of P19 cells: What an embryonal carcinoma cell line taught us about cardiomyocyte differentiation. Cardiovasc Res. 58:292–302. 2003. View Article : Google Scholar : PubMed/NCBI

34 

van der Heyden MA, van Kempen MJ, Tsuji Y, Rook MB, Jongsma HJ and Opthof T: P19 embryonal carcinoma cells: A suitable model system for cardiac electrophysiological differentiation at the molecular and functional level. Cardiovasc Res. 58:410–422. 2003. View Article : Google Scholar : PubMed/NCBI

35 

Qin DN, Qian L, Hu DL, Yu ZB, Han SP, Zhu C, Wang X and Hu X: Effects of miR-19b overexpression on proliferation, differentiation, apoptosis and Wnt/β-catenin signaling pathway in P19 cell model of cardiac differentiation in vitro. Cell Biochem Biophys. 66:709–722. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Zhu C, Hu DL, Liu YQ, Zhang QJ, Chen FK, Kong XQ, Cao KJ, Zhang JS and Qian LM: Fabp3 inhibits proliferation and promotes apoptosis of embryonic myocardial cells. Cell Biochem Biophys. 60:259–266. 2011. View Article : Google Scholar : PubMed/NCBI

37 

Zhu S, Hu X, Han S, Yu Z, Peng Y, Zhu J, Liu X, Qian L, Zhu C and Li M: et al Differential expression profile of long non-coding RNAs during differentiation of cardiomyocytes. Int J Med Sci. 11:500–507. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Yuan S, Zaidi S and Brueckner M: Congenital heart disease: Emerging themes linking genetics and development. Curr Opin Genet Dev. 23:352–359. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Staudt D and Stainier D: Uncovering the molecular and cellular mechanisms of heart development using the zebrafish. Annu Rev Genet. 46:397–418. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Olson EN: Gene regulatory networks in the evolution and development of the heart. Science. 313:1922–1927. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Penton AL, Leonard LD and Spinner NB: Notch signaling in human development and disease. Semin Cell Dev Biol. 23:450–457. 2012. View Article : Google Scholar : PubMed/NCBI

42 

de la Pompa JL: Notch signaling in cardiac development and disease. Pediatr Cardiol. 30:643–650. 2009. View Article : Google Scholar : PubMed/NCBI

43 

McDaniell R, Warthen DM, Sanchez-Lara PA, Pai A, Krantz ID, Piccoli DA and Spinner NB: NOTCH2 mutations cause Alagille syndrome, a heterogeneous disorder of the notch signaling pathway. Am J Hum Genet. 79:169–173. 2006. View Article : Google Scholar : PubMed/NCBI

44 

Kamath BM, Spinner NB, Emerick KM, Chudley AE, Booth C, Piccoli DA and Krantz ID: Vascular anomalies in Alagille syndrome: A significant cause of morbidity and mortality. Circulation. 109:1354–1358. 2004. View Article : Google Scholar : PubMed/NCBI

45 

Garg V, Muth AN, Ransom JF, Schluterman MK, Barnes R, King IN, Grossfeld PD and Srivastava D: Mutations in NOTCH1 cause aortic valve disease. Nature. 437:270–274. 2005. View Article : Google Scholar : PubMed/NCBI

46 

Zhou XL and Liu JC: Role of Notch signaling in the mammalian heart. Braz J Med Biol Res. 47:1–10. 2014. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Wang L, Song G, Liu M, Chen B, Chen Y, Shen Y, Zhu J and Zhou X: MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway. Int J Mol Med 37: 47-55, 2016.
APA
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y. ... Zhou, X. (2016). MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway. International Journal of Molecular Medicine, 37, 47-55. https://doi.org/10.3892/ijmm.2015.2399
MLA
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y., Zhu, J., Zhou, X."MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway". International Journal of Molecular Medicine 37.1 (2016): 47-55.
Chicago
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y., Zhu, J., Zhou, X."MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway". International Journal of Molecular Medicine 37, no. 1 (2016): 47-55. https://doi.org/10.3892/ijmm.2015.2399
Copy and paste a formatted citation
x
Spandidos Publications style
Wang L, Song G, Liu M, Chen B, Chen Y, Shen Y, Zhu J and Zhou X: MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway. Int J Mol Med 37: 47-55, 2016.
APA
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y. ... Zhou, X. (2016). MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway. International Journal of Molecular Medicine, 37, 47-55. https://doi.org/10.3892/ijmm.2015.2399
MLA
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y., Zhu, J., Zhou, X."MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway". International Journal of Molecular Medicine 37.1 (2016): 47-55.
Chicago
Wang, L., Song, G., Liu, M., Chen, B., Chen, Y., Shen, Y., Zhu, J., Zhou, X."MicroRNA-375 overexpression influences P19 cell proliferation, apoptosis and differentiation through the Notch signaling pathway". International Journal of Molecular Medicine 37, no. 1 (2016): 47-55. https://doi.org/10.3892/ijmm.2015.2399
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