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International Journal of Molecular Medicine
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Print ISSN: 1107-3756 Online ISSN: 1791-244X
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January-2016 Volume 37 Issue 1

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Article Open Access

Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress

  • Authors:
    • Ki Cheon Kim
    • Mei Jing Piao
    • Susara Ruwan Kumara Madduma Hewage
    • Xia Han
    • Kyoung Ah Kang
    • Jin Oh Jo
    • Young Sun Mok
    • Jennifer H. Shin
    • Yeunsoo Park
    • Suk Jae Yoo
    • Jin Won Hyun

  • View Affiliations / Copyright

    Affiliations: School of Medicine and Institute for Nuclear Science and Technology, Jeju National University, Jeju 63243, Republic of Korea, Department of Chemical and Biological Engineering, Jeju National University, Jeju 63243, Republic of Korea, Department of Mechanical Engineering and Graduate School of Medical Science and Engineering, KAIST, Daejeon 34141, Republic of Korea, National Fusion Research Institute, Plasma Technology Research Center, Gunsan 54004, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 29-38
    |
    Published online on: November 9, 2015
       https://doi.org/10.3892/ijmm.2015.2405
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Abstract

The aim of this study was to identify the mechanisms through which dielectric-barrier discharge plasma damages human keratinocytes (HaCaT cells) through the induction of oxidative stress. For this purpose, the cells were exposed to surface dielectric-barrier discharge plasma in 70% oxygen and 30% argon. We noted that cell viability was decreased following exposure of the cells to plasma in a time-dependent manner, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The levels of intracellular reactive oxygen species (ROS) were determined using 2',7'-dichlorodihydrofluorescein diacetate and dihydroethidium was used to monitor superoxide anion production. Plasma induced the generation of ROS, including superoxide anions, hydrogen peroxide and hydroxyl radicals. N-acetyl cysteine, which is an antioxidant, prevented the decrease in cell viability caused by exposure to plasma. ROS generated by exposure to plasma resulted in damage to various cellular components, including lipid membrane peroxidation, DNA breaks and protein carbonylation, which was detected by measuring the levels of 8-isoprostane and diphenyl-1-pyrenylphosphine assay, comet assay and protein carbonyl formation. These results suggest that plasma exerts cytotoxic effects by causing oxidative stress-induced damage to cellular components.

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Copy and paste a formatted citation
Spandidos Publications style
Kim KC, Piao MJ, Madduma Hewage SR, Han X, Kang KA, Jo JO, Mok YS, Shin JH, Park Y, Yoo SJ, Yoo SJ, et al: Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress. Int J Mol Med 37: 29-38, 2016.
APA
Kim, K.C., Piao, M.J., Madduma Hewage, S.R., Han, X., Kang, K.A., Jo, J.O. ... Hyun, J.W. (2016). Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress. International Journal of Molecular Medicine, 37, 29-38. https://doi.org/10.3892/ijmm.2015.2405
MLA
Kim, K. C., Piao, M. J., Madduma Hewage, S. R., Han, X., Kang, K. A., Jo, J. O., Mok, Y. S., Shin, J. H., Park, Y., Yoo, S. J., Hyun, J. W."Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress". International Journal of Molecular Medicine 37.1 (2016): 29-38.
Chicago
Kim, K. C., Piao, M. J., Madduma Hewage, S. R., Han, X., Kang, K. A., Jo, J. O., Mok, Y. S., Shin, J. H., Park, Y., Yoo, S. J., Hyun, J. W."Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress". International Journal of Molecular Medicine 37, no. 1 (2016): 29-38. https://doi.org/10.3892/ijmm.2015.2405
Copy and paste a formatted citation
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Spandidos Publications style
Kim KC, Piao MJ, Madduma Hewage SR, Han X, Kang KA, Jo JO, Mok YS, Shin JH, Park Y, Yoo SJ, Yoo SJ, et al: Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress. Int J Mol Med 37: 29-38, 2016.
APA
Kim, K.C., Piao, M.J., Madduma Hewage, S.R., Han, X., Kang, K.A., Jo, J.O. ... Hyun, J.W. (2016). Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress. International Journal of Molecular Medicine, 37, 29-38. https://doi.org/10.3892/ijmm.2015.2405
MLA
Kim, K. C., Piao, M. J., Madduma Hewage, S. R., Han, X., Kang, K. A., Jo, J. O., Mok, Y. S., Shin, J. H., Park, Y., Yoo, S. J., Hyun, J. W."Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress". International Journal of Molecular Medicine 37.1 (2016): 29-38.
Chicago
Kim, K. C., Piao, M. J., Madduma Hewage, S. R., Han, X., Kang, K. A., Jo, J. O., Mok, Y. S., Shin, J. H., Park, Y., Yoo, S. J., Hyun, J. W."Non-thermal dielectric-barrier discharge plasma damages human keratinocytes by inducing oxidative stress". International Journal of Molecular Medicine 37, no. 1 (2016): 29-38. https://doi.org/10.3892/ijmm.2015.2405
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