Open Access

The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress

  • Authors:
    • Shu Tang
    • Hongbo Chen
    • Yanfen Cheng
    • Mohammad Abdel Nasir
    • Nicole Kemper
    • Endong Bao
  • View Affiliations

  • Published online on: November 19, 2015     https://doi.org/10.3892/ijmm.2015.2414
  • Pages: 56-62
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Heat shock factor 1 (HSF1) is a heat shock transcription factor that rapidly induces heat shock gene transcription following thermal stress. In this study, we subjected primary neonatal rat myocardial cells to heat stress in vitro to create a model system for investigating the trends in expression and association between various heat shock proteins (HSPs) and HSF1 under adverse environmental conditions. After the cells were subjected to heat stress at 42˚C for different periods of time, HSP and HSF1 mRNA and protein levels were detected by qPCR and western blot analysis in the heat-stressed cells. The HSF1 expression levels significantly increased in the cells following 120 min of exposure to heat stess compared to the levels observed at the beginning of heat stress exposure. HSP90 followed a similar trend in expression to HSF1, whereas HSP70 followed an opposite trend. However, no significant changes were observed in the crystallin, alpha B (CRYAB, also known as HSP beta-5) expression levels during the 480‑min period of exposure to heat stress. The interaction between the HSPs and HSF1 was analyzed by STRING 9.1, and it was found that HSF1 interacted with HSP90 and HSP70, and that it did not play a role in regulating CRYAB expression. Based on our findings, HSP70 may suppress HSF1 in rat myocardial cells under conditions of heat stress. Furthermore, our data demonstrate that HSF1 is not the key factor for all HSPs, and this was particularly the case for CRYAB.
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January-2016
Volume 37 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Tang S, Chen H, Cheng Y, Nasir MA, Kemper N and Bao E: The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress. Int J Mol Med 37: 56-62, 2016
APA
Tang, S., Chen, H., Cheng, Y., Nasir, M.A., Kemper, N., & Bao, E. (2016). The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress. International Journal of Molecular Medicine, 37, 56-62. https://doi.org/10.3892/ijmm.2015.2414
MLA
Tang, S., Chen, H., Cheng, Y., Nasir, M. A., Kemper, N., Bao, E."The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress". International Journal of Molecular Medicine 37.1 (2016): 56-62.
Chicago
Tang, S., Chen, H., Cheng, Y., Nasir, M. A., Kemper, N., Bao, E."The interactive association between heat shock factor 1 and heat shock proteins in primary myocardial cells subjected to heat stress". International Journal of Molecular Medicine 37, no. 1 (2016): 56-62. https://doi.org/10.3892/ijmm.2015.2414