Open Access

In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury

  • Authors:
    • Caterina Constantinou
    • Yiorgos Apidianakis
    • Nikolaos Psychogios
    • Valeria Righi
    • Michael N. Mindrinos
    • Nadeem Khan
    • Harold M. Swartz
    • Hazel H. Szeto
    • Ronald G. Tompkins
    • Laurence G. Rahme
    • A. Aria Tzika
  • View Affiliations

  • Published online on: December 8, 2015     https://doi.org/10.3892/ijmm.2015.2426
  • Pages: 299-308
  • Copyright: © Constantinou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Trauma is the most common cause of mortality among individuals aged between 1 and 44 years and the third leading cause of mortality overall in the US. In this study, we examined the effects of trauma on the expression of genes in Drosophila melanogaster, a useful model for investigating genetics and physiology. After trauma was induced by a non-lethal needle puncture of the thorax, we observed the differential expression of genes encoding for mitochondrial uncoupling proteins, as well as those encoding for apoptosis-related and insulin signaling-related proteins, thus indicating muscle functional dysregulation. These results prompted us to examine the link between insulin signaling and mitochondrial dysfunction using in vivo nuclear magnetic resonance (NMR) with complementary electron paramagnetic resonance (EPR) spectroscopy. Trauma significantly increased insulin resistance biomarkers, and the NMR spectral profile of the aged flies with trauma-induced thoracic injury resembled that of insulin-resistant chico mutant flies. In addition, the mitochondrial redox status, as measured by EPR, was significantly altered following trauma, indicating mitochondrial uncoupling. A mitochondria-targeted compound, Szeto-Schiller (SS)-31 that promotes adenosine triphosphate (ATP) synthesis normalized the NMR spectral profile, as well as the mitochondrial redox status of the flies with trauma-induced thoracic injury, as assessed by EPR. Based on these findings, we propose a molecular mechanism responsible for trauma-related mortality and also propose that trauma sequelae in aging are linked to insulin signaling and mitochondrial dysfunction. Our findings further suggest that SS-31 attenuates trauma-associated pathological changes.
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February-2016
Volume 37 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Constantinou C, Apidianakis Y, Psychogios N, Righi V, Mindrinos MN, Khan N, Swartz HM, Szeto HH, Tompkins RG, Rahme LG, Rahme LG, et al: In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury. Int J Mol Med 37: 299-308, 2016
APA
Constantinou, C., Apidianakis, Y., Psychogios, N., Righi, V., Mindrinos, M.N., Khan, N. ... Tzika, A.A. (2016). In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury. International Journal of Molecular Medicine, 37, 299-308. https://doi.org/10.3892/ijmm.2015.2426
MLA
Constantinou, C., Apidianakis, Y., Psychogios, N., Righi, V., Mindrinos, M. N., Khan, N., Swartz, H. M., Szeto, H. H., Tompkins, R. G., Rahme, L. G., Tzika, A. A."In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury". International Journal of Molecular Medicine 37.2 (2016): 299-308.
Chicago
Constantinou, C., Apidianakis, Y., Psychogios, N., Righi, V., Mindrinos, M. N., Khan, N., Swartz, H. M., Szeto, H. H., Tompkins, R. G., Rahme, L. G., Tzika, A. A."In vivo high-resolution magic angle spinning magnetic and electron paramagnetic resonance spectroscopic analysis of mitochondria-targeted peptide in Drosophila melanogaster with trauma-induced thoracic injury". International Journal of Molecular Medicine 37, no. 2 (2016): 299-308. https://doi.org/10.3892/ijmm.2015.2426