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Article

miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein

  • Authors:
    • Jiantao Sun
    • Zhiqiang Fan
    • Shuaiqi Lu
    • Jinhui Yang
    • Tongtong Hao
    • Qingxiang Huo
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, Henan 471002, P.R. China, Department of Urology, Henan Provincial People's Hospital, Zhengzhou, Henan 450003, P.R. China
  • Pages: 485-492
    |
    Published online on: January 5, 2016
       https://doi.org/10.3892/ijmm.2016.2449
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Abstract

Nin one binding (NOB1) protein has been identified as an oncogene in various human cancers, including prostate cancer. MicroRNAs (miRs) have also been recognized as novel regulatory molecules of gene expression. The present study aimed to discover potential miRs that target NOB1 and regulate NOB1 expression in prostate cancer. miR-192, which is an important regulator of numerous cancers, was found to be significantly downregulated in prostate cancer cells. Moreover, we noted that miR-192 overexpression markedly inhibited the proliferation, colony‑forming ability, and migratory capacity of the prostate cancer cells. miR-192 overexpression also induced cell cycle arrest in the G1 phase, as shown by flow cytometry. Bioinformatics analysis results revealed that NOB1 was a possible candidate target gene of miR-192. This discovery was further verified through dual‑luciferase reporter assay, RT-qPCR, and western blot analysis. We suggest that miR-192 directly regulates the mRNA and protein expression of NOB1. Furthermore, miR-192 inhibited the expression of p38 mitogen-activated protein kinase. The results of our study indicated that miR-192 negatively regulated NOB1 expression and impaired the tumorigenicity of prostate cancer cells. Therefore, we suggest that targeting miR-192 and NOB1 is a novel strategy which will assist in the development of new therapeutics that will be used in the future to prevent and treat prostate cancer.
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1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Roy M, Kung HJ and Ghosh PM: Statins and prostate cancer: role of cholesterol inhibition vs. prevention of small GTP-binding proteins. Am J Cancer Res. 1:542–561. 2011.PubMed/NCBI

3 

Wang H, Li P and Zhao B: Knockdown of NOB1 expression by RNAi inhibits cellular proliferation and migration in human gliomas. Gene. 528:146–153. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Lin Y, Peng S, Yu H, Teng H and Cui M: RNAi-mediated down-regulation of NOB1 suppresses the growth and colony-formation ability of human ovarian cancer cells. Med Oncol. 29:311–317. 2012. View Article : Google Scholar

5 

Zhang Y, Ni J, Zhou G, Yuan J, Ren W, Shan Y, Tang W, Yu L and Zhao S: Cloning, expression and characterization of the human NOB1 gene. Mol Biol Rep. 32:185–189. 2005. View Article : Google Scholar : PubMed/NCBI

6 

Veith T, Martin R, Wurm JP, Weis BL, Duchardt-Ferner E, Safferthal C, Hennig R, Mirus O, Bohnsack MT, Wöhnert J and Schleiff E: Structural and functional analysis of the archaeal endonuclease Nob1. Nucleic Acids Res. 40:3259–3274. 2012. View Article : Google Scholar :

7 

Lu Z, Guo Q, Shi A, Xie F and Lu Q: Downregulation of NIN/RPN12 binding protein inhibit the growth of human hepatocellular carcinoma cells. Mol Biol Rep. 39:501–507. 2012. View Article : Google Scholar

8 

Huang WY, Chen DH, Ning L and Wang LW: siRNA mediated silencing of NIN1/RPN12 binding protein 1 homolog inhibits proliferation and growth of breast cancer cells. Asian Pac J Cancer Prev. 13:1823–1827. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Lin S, Meng W, Zhang W, Liu J, Wang P, Xue S and Chen G: Expression of the NOB1 gene and its clinical significance in papillary thyroid carcinoma. J Int Med Res. 41:568–572. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li Y, Ma C, Qian M, Wen Z, Jing H and Qian D: Downregulation of NOB1 suppresses the proliferation and tumor growth of non-small cell lung cancer in vitro and in vivo. Oncol Rep. 31:1271–1276. 2014.PubMed/NCBI

11 

Che JP, Li W, Yan Y, Liu M, Wang GC, Li QY, Yang B, Yao XD and Zheng JH: Expression and clinical significance of the nin one binding protein and p38 MAPK in prostate carcinoma. Int J Clin Exp Pathol. 6:2300–2311. 2013.PubMed/NCBI

12 

Liu G, Shen D, Jiao L and Sun Y: Nin one binding protein expression as a prognostic marker in prostate carcinoma. Clin Transl Oncol. 16:843–847. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Liu K, Chen HL, Gu MM and You QS: Relationship between NOB1 expression and prognosis of resected non-small cell lung cancer. Int J Biol Markers. 30:e43–e48. 2015. View Article : Google Scholar

14 

Zhang X, Zhang D, Qu F, Hong Y, Cao J, Pan X, Li L, Huang Y, Huang H, Yin L, et al: Knockdown of NOB1 expression inhibits the malignant transformation of human prostate cancer cells. Mol Cell Biochem. 396:1–8. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Bartel DP: MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Winter J, Jung S, Keller S, Gregory RI and Diederichs S: Many roads to maturity: microRNA biogenesis pathways and their regulation. Nat Cell Biol. 11:228–234. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Mendell JT and Olson EN: MicroRNAs in stress signaling and human disease. Cell. 148:1172–1187. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Ranganathan K and Sivasankar V: MicroRNAs - Biology and clinical applications. J Oral Maxillofac Pathol. 18:229–234. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Wang YL, Wu S, Jiang B, Yin FF, Zheng SS and Hou SC: Role of microRNAs in prostate cancer pathogenesis. Clin Genitourin Cancer. 13:261–270. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Geng L, Chaudhuri A, Talmon G, Wisecarver JL, Are C, Brattain M and Wang J: MicroRNA-192 suppresses liver metastasis of colon cancer. Oncogene. 33:5332–5340. 2014. View Article : Google Scholar :

21 

Jin Y, Lu J, Wen J, Shen Y and Wen X: Regulation of growth of human bladder cancer by miR-192. Tumour Biol. 36:3791–3797. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Goto Y, Kojima S, Nishikawa R, Enokida H, Chiyomaru T, Kinoshita T, Nakagawa M, Naya Y, Ichikawa T and Seki N: The microRNA-23b/27b/24-1 cluster is a disease progression marker and tumor suppressor in prostate cancer. Oncotarget. 5:7748–7759. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Augello C, Vaira V, Caruso L, Destro A, Maggioni M, Park YN, Montorsi M, Santambrogio R, Roncalli M and Bosari S: MicroRNA profiling of hepatocarcinogenesis identifies C19MC cluster as a novel prognostic biomarker in hepatocellular carcinoma. Liver Int. 32:772–782. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Liu J, Dong BF, Wang PS, Ren PY, Xue S, Zhang X, Han Z and Chen G: Silencing NOB1 enhances doxorubicin antitumor activity of the papillary thyroid carcinoma in vitro and in vivo. Oncol Rep. 33:1551–1559. 2015.PubMed/NCBI

25 

Liu K, Gu MM, Chen HL and You QS: NOB1 in non-small-cell lung cancer: expression profile and clinical significance. Pathol Oncol Res. 20:461–466. 2014. View Article : Google Scholar

26 

Liu K, Chen HL, Gu MM and You QS: NOB1 expression predicts early response to Cisplatin-based chemotherapy in patients with advanced non-small cell lung cancer. J Chemother. May 13–2015.Epub ahead of print. View Article : Google Scholar

27 

Chen B, Liu J, Wu D, Qin Y, Peng C, Li C and Wang J: Gene silencing of NOB1 by lentivirus suppresses growth and migration of human osteosarcoma cells. Mol Med Rep. 9:2173–2179. 2014.PubMed/NCBI

28 

Liu Y, Huang H, Yuan B, Zhuang LY, Luo TP and Zhang Q: Lentivirus-mediated knockdown of NOB1 suppresses the proliferation of colon cancer cells. Z Gastroenterol. 52:429–435. 2014. View Article : Google Scholar : PubMed/NCBI

29 

He XW, Feng T, Yin QL, Jian YW and Liu T: NOB1 is essential for the survival of RKO colorectal cancer cells. World J Gastroenterol. 21:868–877. 2015.PubMed/NCBI

30 

Jia JW, Liu AQ, Wang Y, Zhao F, Jiao LL and Tan J: Evaluation of NIN/RPN12 binding protein inhibits proliferation and growth in human renal cancer cells. Tumour Biol. 36:1803–1810. 2015. View Article : Google Scholar

31 

Chen J, Wang J, Cui X, Liu Y, Yin L, Li Y, Chen L, Xu D and Gao Y: Positive nin one binding protein expression predicts poor outcome in prostate cancer. Mol Med Rep. 11:2671–2676. 2015.

32 

Xu YJ and Fan Y: MiR-215/192 participates in gastric cancer progression. Clin Transl Oncol. 17:34–40. 2015. View Article : Google Scholar

33 

Feinberg-Gorenshtein G, Guedj A, Shichrur K, Jeison M, Luria D, Kodman Y, Ash S, Feinmesser M, Edry L, Shomron N, et al: MiR-192 directly binds and regulates Dicer1 expression in neuroblastoma. PLoS One. 8:e787132013. View Article : Google Scholar : PubMed/NCBI

34 

Feng S, Cong S, Zhang X, Bao X, Wang W, Li H, Wang Z, Wang G, Xu J, Du B, et al: MicroRNA-192 targeting retinoblastoma 1 inhibits cell proliferation and induces cell apoptosis in lung cancer cells. Nucleic Acids Res. 39:6669–6678. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Zhou J, Xu T, Yan Y, Qin R, Wang H, Zhang X, Huang Y, Wang Y, Lu Y, Fu D and Chen J: MicroRNA-326 functions as a tumor suppressor in glioma by targeting the Nin one binding protein (NOB1). PLoS One. 8:e684692013. View Article : Google Scholar : PubMed/NCBI

36 

Wu L, Hui H, Wang LJ, Wang H, Liu QF and Han SX: MicroRNA-326 functions as a tumor suppressor in colorectal cancer by targeting the nin one binding protein. Oncol Rep. 33:2309–2318. 2015.PubMed/NCBI

37 

Li W, Liu M, Feng Y, Xu YF, Huang YF, Che JP, Wang GC, Yao XD and Zheng JH: Downregulated miR-646 in clear cell renal carcinoma correlated with tumour metastasis by targeting the nin one binding protein (NOB1). Br J Cancer. 111:1188–1200. 2014. View Article : Google Scholar : PubMed/NCBI

38 

Li XD, Liu ZY, Chang B, Liu DX, Chen B, Guo C, Wang YG, Xu JK, Huang DY and Du SX: Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways. Cell Physiol Biochem. 28:367–376. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Sun J, Fan Z, Lu S, Yang J, Hao T and Huo Q: miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein. Int J Mol Med 37: 485-492, 2016.
APA
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., & Huo, Q. (2016). miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein. International Journal of Molecular Medicine, 37, 485-492. https://doi.org/10.3892/ijmm.2016.2449
MLA
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., Huo, Q."miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein". International Journal of Molecular Medicine 37.2 (2016): 485-492.
Chicago
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., Huo, Q."miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein". International Journal of Molecular Medicine 37, no. 2 (2016): 485-492. https://doi.org/10.3892/ijmm.2016.2449
Copy and paste a formatted citation
x
Spandidos Publications style
Sun J, Fan Z, Lu S, Yang J, Hao T and Huo Q: miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein. Int J Mol Med 37: 485-492, 2016.
APA
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., & Huo, Q. (2016). miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein. International Journal of Molecular Medicine, 37, 485-492. https://doi.org/10.3892/ijmm.2016.2449
MLA
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., Huo, Q."miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein". International Journal of Molecular Medicine 37.2 (2016): 485-492.
Chicago
Sun, J., Fan, Z., Lu, S., Yang, J., Hao, T., Huo, Q."miR-192 suppresses the tumorigenicity of prostate cancer cells by targeting and inhibiting nin one binding protein". International Journal of Molecular Medicine 37, no. 2 (2016): 485-492. https://doi.org/10.3892/ijmm.2016.2449
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