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International Journal of Molecular Medicine
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Article

Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism

  • Authors:
    • Eva Juengel
    • Masud Afschar
    • Jasmina Makarević
    • Jochen Rutz
    • Igor Tsaur
    • Jens Mani
    • Karen Nelson
    • Axel Haferkamp
    • Roman A. Blaheta
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Goethe-University Hospital, Frankfurt am Main, Germany, Department of Vascular and Endovascular Surgery, Goethe-University Hospital, Frankfurt am Main, Germany
  • Pages: 843-850
    |
    Published online on: January 8, 2016
       https://doi.org/10.3892/ijmm.2016.2454
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Abstract

Information about the natural compound amygdalin, which is employed as an antitumor agent, is sparse and thus its efficacy remains controversial. In this study, to determine whether amygdalin exerts antitumor effects on renal cell carcinoma (RCC) cells, its impact on RCC metastatic activity was investigated. The RCC cell lines, Caki-1, KTC-26 and A498, were exposed to amygdalin from apricot kernels, and adhesion to human vascular endothelium, immobilized collagen or fibronectin was investigated. The influence of amygdalin on chemotactic and invasive activity was also determined, as was the influence of amygdalin on surface and total cellular α and β integrin expression, which are involved in metastasis. We noted that amygdalin caused significant reductions in chemotactic activity, invasion and adhesion to endothelium, collagen and fibronectin. Using FACScan analysis, we noted that amygdalin also induced reductions, particularly in integrins α5 and α6, in all three cell lines. Functional blocking of α5 resulted in significantly diminished adhesion of KTC-26 and A498 to collagen and also in decreased chemotactic behavior in all three cell lines. Blocking α6 integrin significantly reduced chemotactic activity in all three cell lines. Thus, we suggest that exposing RCC cells to amygdalin inhibits metastatic spread and is associated with downregulation of α5 and α6 integrins. Therefore, we posit that amygdalin exerts antitumor activity in vitro, and this may be linked to integrin regulation.
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Copy and paste a formatted citation
Spandidos Publications style
Juengel E, Afschar M, Makarević J, Rutz J, Tsaur I, Mani J, Nelson K, Haferkamp A and Blaheta RA: Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism. Int J Mol Med 37: 843-850, 2016.
APA
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J. ... Blaheta, R.A. (2016). Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism. International Journal of Molecular Medicine, 37, 843-850. https://doi.org/10.3892/ijmm.2016.2454
MLA
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J., Nelson, K., Haferkamp, A., Blaheta, R. A."Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism". International Journal of Molecular Medicine 37.3 (2016): 843-850.
Chicago
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J., Nelson, K., Haferkamp, A., Blaheta, R. A."Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism". International Journal of Molecular Medicine 37, no. 3 (2016): 843-850. https://doi.org/10.3892/ijmm.2016.2454
Copy and paste a formatted citation
x
Spandidos Publications style
Juengel E, Afschar M, Makarević J, Rutz J, Tsaur I, Mani J, Nelson K, Haferkamp A and Blaheta RA: Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism. Int J Mol Med 37: 843-850, 2016.
APA
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J. ... Blaheta, R.A. (2016). Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism. International Journal of Molecular Medicine, 37, 843-850. https://doi.org/10.3892/ijmm.2016.2454
MLA
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J., Nelson, K., Haferkamp, A., Blaheta, R. A."Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism". International Journal of Molecular Medicine 37.3 (2016): 843-850.
Chicago
Juengel, E., Afschar, M., Makarević, J., Rutz, J., Tsaur, I., Mani, J., Nelson, K., Haferkamp, A., Blaheta, R. A."Amygdalin blocks the in vitro adhesion and invasion of renal cell carcinoma cells by an integrin-dependent mechanism". International Journal of Molecular Medicine 37, no. 3 (2016): 843-850. https://doi.org/10.3892/ijmm.2016.2454
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