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International Journal of Molecular Medicine
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Article

Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading

  • Authors:
    • Qiannan Niu
    • Feifei Li
    • Liang Zhang
    • Xinyuan Xu
    • Yucong Liu
    • Jie Gao
    • Xue Feng
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Military Stomatology, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Stomatology, Hospital 323 of The People's Liberation Army, Xi'an, Shaanxi 710045, P.R. China, Department of Biochemistry and Molecular Biology, School of Stomatology, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Stomatology, The First People's Hospital of Shuangliu County, Chengdu, Sichuan 610200, P.R. China
  • Pages: 755-762
    |
    Published online on: January 22, 2016
       https://doi.org/10.3892/ijmm.2016.2463
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Abstract

In order to better understand the mechanisms by which chondrocytes respond to mechanical stimulation, ATDC5 mouse embryonic carcinoma cells were induced to differentiate into chondrocytes and then exposed to mechanical loading. To specifically elucidate the role of this pathway, the localization and expression of proteins involved in the Wnt/β-catenin signaling pathway were observed. Chondrogenic-differentiated ATDC5 cells were exposed to a 12% cycle tension load for 1, 2, 4, or 8 h. At each time point, immunofluorescence staining, western blot analysis, and qPCR were used to track the localization of β-catenin and glycogen synthase kinase-3β (GSK-3β) expression. In addition, the mRNA expression of Wnt3a, disheveled homolog 1 (Dvl-1), GSK-3β, and collagen type II were also detected. Activation of the Wnt/β-catenin signaling pathway was investigated in cells treated with Dickkopf-related protein 1 (DKK-1). β-catenin and GSK-3β protein expression increased initially and then decreased over the mechanical loading period, and the corresponding mRNA levels followed a similar trend. After application of the inhibitor DKK-1, Wnt/β‑catenin signaling was suppressed, and the mRNA expression of collagen II was also reduced. Thus, stimulation of chondrocytes with mechanical strain loading is associated with the translocation of active β-catenin from the cytoplasm to the nucleus.
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Copy and paste a formatted citation
Spandidos Publications style
Niu Q, Li F, Zhang L, Xu X, Liu Y, Gao J and Feng X: Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading. Int J Mol Med 37: 755-762, 2016.
APA
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., & Feng, X. (2016). Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading. International Journal of Molecular Medicine, 37, 755-762. https://doi.org/10.3892/ijmm.2016.2463
MLA
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., Feng, X."Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading". International Journal of Molecular Medicine 37.3 (2016): 755-762.
Chicago
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., Feng, X."Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading". International Journal of Molecular Medicine 37, no. 3 (2016): 755-762. https://doi.org/10.3892/ijmm.2016.2463
Copy and paste a formatted citation
x
Spandidos Publications style
Niu Q, Li F, Zhang L, Xu X, Liu Y, Gao J and Feng X: Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading. Int J Mol Med 37: 755-762, 2016.
APA
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., & Feng, X. (2016). Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading. International Journal of Molecular Medicine, 37, 755-762. https://doi.org/10.3892/ijmm.2016.2463
MLA
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., Feng, X."Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading". International Journal of Molecular Medicine 37.3 (2016): 755-762.
Chicago
Niu, Q., Li, F., Zhang, L., Xu, X., Liu, Y., Gao, J., Feng, X."Role of the Wnt/β-catenin signaling pathway in the response of chondrocytes to mechanical loading". International Journal of Molecular Medicine 37, no. 3 (2016): 755-762. https://doi.org/10.3892/ijmm.2016.2463
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