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Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells

  • Authors:
    • Hui-Ping Huang
    • Wen-Jun Liu
    • Qu-Lian Guo
    • Yong-Qi Bai
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The First Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 646000, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 669-678
    |
    Published online on: February 4, 2016
       https://doi.org/10.3892/ijmm.2016.2480
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Abstract

Acute lymphocytic leukemia (ALL) is a common malignant tumor with a high morbidity rate among children, accounting for approximately 80% of leukemia cases. Although there have been improvements in the treatment of patients frequent relapse lead to a poor prognosis. The aim of the present study was to determine whether HOXA5 may be used as a target for gene therapy in leukemia in order to provide a new treatment. Mononuclear cells were extracted from the bone marrow according to the clinical research aims. After testing for ALL in the acute stage, the relative mRNA and protein expression of HOXA5 was detected in the ALL remission groups (n=25 cases per group) and the control group [n=20 cases, immune thrombocytopenia (ITP)]. Gene silencing by RNA interference (RNAi) was used to investigate the effect of silencing HOXA5 after small interfering RNA (siRNA) transfection to Jurkat cells. The HOXA5-specific siRNA was transfected to Jurkat cells using lipofectamine. The experiment was divided into the experimental group (liposomal transfection of HOXA5 targeting siRNA), the negative control group (liposomal transfection of cells with negative control siRNA) and the control group (plus an equal amount of cells and culture media only). Western blotting and quantitative fluorescent polymerase chain reaction (QF‑PCR) were used to detect the relative HOXA5 mRNA expression and protein distribution in each cell group. Cell distribution in the cell cycle and the rate of cells undergoing apoptosis were determined using flow cytometry. The expression of HOXA5 at the mRNA and protein levels in the acute phase of ALL was significantly higher than that in ALL in the remission and control groups. In cells transfected with HOXA5-specific siRNA, the expression of HOXA5 at the mRNA and protein levels decreased significantly (P<0.05). The distribution of cells in the cell cycle was also altered. Specifically, more cells were present in the G0/G1 phase compared to the S phase (P<0.05). In addition, the apoptotic rate was significantly higher in cells transfected with HOXA5‑specific siRNA (P<0.05). In conclusion, high expression levels of HOXA5 mRNA and protein in children with ALL indicate that HOXA5 is closely associated with childhood ALL. In addition, HOXA5-specific siRNA effectively silences HOXA5 gene expression and induces apoptosis and cell-cycle arrest in Jurkat cells, thus inhibiting cell proliferation.
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Copy and paste a formatted citation
Spandidos Publications style
Huang H, Liu W, Guo Q and Bai Y: Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells. Int J Mol Med 37: 669-678, 2016.
APA
Huang, H., Liu, W., Guo, Q., & Bai, Y. (2016). Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells. International Journal of Molecular Medicine, 37, 669-678. https://doi.org/10.3892/ijmm.2016.2480
MLA
Huang, H., Liu, W., Guo, Q., Bai, Y."Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells". International Journal of Molecular Medicine 37.3 (2016): 669-678.
Chicago
Huang, H., Liu, W., Guo, Q., Bai, Y."Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells". International Journal of Molecular Medicine 37, no. 3 (2016): 669-678. https://doi.org/10.3892/ijmm.2016.2480
Copy and paste a formatted citation
x
Spandidos Publications style
Huang H, Liu W, Guo Q and Bai Y: Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells. Int J Mol Med 37: 669-678, 2016.
APA
Huang, H., Liu, W., Guo, Q., & Bai, Y. (2016). Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells. International Journal of Molecular Medicine, 37, 669-678. https://doi.org/10.3892/ijmm.2016.2480
MLA
Huang, H., Liu, W., Guo, Q., Bai, Y."Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells". International Journal of Molecular Medicine 37.3 (2016): 669-678.
Chicago
Huang, H., Liu, W., Guo, Q., Bai, Y."Effect of silencing HOXA5 gene expression using RNA interference on cell cycle and apoptosis in Jurkat cells". International Journal of Molecular Medicine 37, no. 3 (2016): 669-678. https://doi.org/10.3892/ijmm.2016.2480
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