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Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis

  • Authors:
    • Magdy El-Salhy
    • Kazuo Umezawa
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology, Department of Medicine, Stord Helse-Fonna Hospital, Stord, Norway, Department of Molecular Target Medicine, School of Aichi Medical University, School of Medicine, Nagakute, Aichi, Japan
    Copyright: © El-Salhy et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 556-564
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    Published online on: February 5, 2016
       https://doi.org/10.3892/ijmm.2016.2481
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Abstract

The aim of this study was to determine the effects of two anti-inflammatory agents on the abnormalities in colonic endocrine cells in dextran sodium sulfate (DSS)-induced colitis. Colitis was induced in male Wistar rats (n=45) using DSS; a further 15 rats without colitis were included in a healthy control group. The animals with DSS-induced colitis were randomly divided into 3 treatment groups as follows: i) DSS group, rats were treated with 0.5 ml of 0.5% carboxymethyl cellulose (CMC); ii) DSS‑G group, rats were treated with 3-[(dodecylthiocarbonyl)‑methyl]‑glutarimide (DTCM‑G), a novel activator protein 1 (AP-1) inhibitor, 20 mg/kg in CMC; and iii) DSS‑Q group, rats were treated with dehydroxymethylepoxyquinomicin, a nuclear factor κB (NF-κB) inhibitor, 15 mg/kg in CMC. The treatments were administered intraperitoneally, twice daily for 5 days, after which the animals were sacrificed and tissue samples from the colon were immunostained for chromogranin A (CgA), serotonin, peptide YY (PYY), enteroglucagon, pancreatic polypeptide (PP), somatostatin, leukocytes, B/T lymphocytes, B lymphocytes, T lymphocytes, macrophages/monocytes and mast cells. The densities of these endocrine and immune cells were quantified by computer‑aided image analysis. The densities of CgA-, serotonin-, PYY- and enteroglucagon-producing cells were significantly higher, and those of PP- and somatostatin-producing cells were significantly lower in the DSS‑G, DSS‑Q and control groups than in the DSS group. The densities of all the immune cells were lower in the DSS‑G, DSS‑Q and control groups than in the DSS group. The densities of all endocrine cell types and immune cells in both the DSS groups treated with anti‑inflammatory agents were restored to control levels. In conclusion, our data demonstrate that there is an interaction between endocrine and immune cells during inflammation. This interaction with subsequent changes in endocrine cells is responsible for the clinical manifestation of colitis symptoms.
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Copy and paste a formatted citation
Spandidos Publications style
El-Salhy M and Umezawa K: Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis. Int J Mol Med 37: 556-564, 2016.
APA
El-Salhy, M., & Umezawa, K. (2016). Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis. International Journal of Molecular Medicine, 37, 556-564. https://doi.org/10.3892/ijmm.2016.2481
MLA
El-Salhy, M., Umezawa, K."Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis". International Journal of Molecular Medicine 37.3 (2016): 556-564.
Chicago
El-Salhy, M., Umezawa, K."Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis". International Journal of Molecular Medicine 37, no. 3 (2016): 556-564. https://doi.org/10.3892/ijmm.2016.2481
Copy and paste a formatted citation
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Spandidos Publications style
El-Salhy M and Umezawa K: Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis. Int J Mol Med 37: 556-564, 2016.
APA
El-Salhy, M., & Umezawa, K. (2016). Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis. International Journal of Molecular Medicine, 37, 556-564. https://doi.org/10.3892/ijmm.2016.2481
MLA
El-Salhy, M., Umezawa, K."Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis". International Journal of Molecular Medicine 37.3 (2016): 556-564.
Chicago
El-Salhy, M., Umezawa, K."Treatment with novel AP-1 and NF-κB inhibitors restores the colonic endocrine cells to normal levels in rats with DSS-induced colitis". International Journal of Molecular Medicine 37, no. 3 (2016): 556-564. https://doi.org/10.3892/ijmm.2016.2481
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