Ginsenoside Rg1 exerts a protective effect against Aβ25-35-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway

  • Authors:
    • Jiaying Wu
    • Hongyu Yang
    • Qingwei Zhao
    • Xingguo Zhang
    • Yijia Lou
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  • Published online on: February 8, 2016     https://doi.org/10.3892/ijmm.2016.2485
  • Pages: 781-788
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Abstract

Ginsenoside Rg1 (Rg1) is a multipotent triterpene saponin extracted from ginseng, and has been proven to act as a nootropic agent against various types of neurological damage. The present study was designed to investigate the neuroprotective effect and the underlying mechanisms of Rg1 on apoptosis induced by β-amyloid peptide 25-35 (Aβ25-35) in primary cultured cortical neurons. The primary neurons were preincubated with 20 µM Rg1 for 24 h and exposed to 10 µM Aβ25-35 for 72 h. In the present study, we found that Rg1 prevented nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) nuclear translocation and IκB-α phosphorylation in primary cultured cortical neurons after Aβ25-35 exposure by scavenging excess reactive oxygen species (ROS); ROS was measured using DCFDA and examined using a fluorescence microscope. In addition, Rg1 successfully suppressed Aβ25‑35-inducible nitric oxide synthase (iNOS) expression and nitric oxide (NO) production in a NF-κB-dependent manner; the suppression of NO was clearly illustrated by the NO production assay. Pretreatment of the cells with Rg1 elevated the proportion of Bcl-2/Bax, lessened the release of cytochrome c from mitochondria into cytoplasm and then blocked mitochondrial apoptotic cascades after Aβ25-35 insult by lowering NO generation. Taken together, our data demonstrate that Rg1 rescues primary cultured cortical neurons from Aβ25-35-induced cell apoptosis through the downregulation of the NF-κB/NO signaling pathway.
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March-2016
Volume 37 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wu J, Yang H, Zhao Q, Zhang X and Lou Y: Ginsenoside Rg1 exerts a protective effect against Aβ25-35-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway. Int J Mol Med 37: 781-788, 2016.
APA
Wu, J., Yang, H., Zhao, Q., Zhang, X., & Lou, Y. (2016). Ginsenoside Rg1 exerts a protective effect against Aβ25-35-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway. International Journal of Molecular Medicine, 37, 781-788. https://doi.org/10.3892/ijmm.2016.2485
MLA
Wu, J., Yang, H., Zhao, Q., Zhang, X., Lou, Y."Ginsenoside Rg1 exerts a protective effect against Aβ25-35-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway". International Journal of Molecular Medicine 37.3 (2016): 781-788.
Chicago
Wu, J., Yang, H., Zhao, Q., Zhang, X., Lou, Y."Ginsenoside Rg1 exerts a protective effect against Aβ25-35-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway". International Journal of Molecular Medicine 37, no. 3 (2016): 781-788. https://doi.org/10.3892/ijmm.2016.2485