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Article

miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin

  • Authors:
    • Hao-Cheng Long
    • Xia Gao
    • Chang-Jiang Lei
    • Bin Zhu
    • Lei Li
    • Cheng Zeng
    • Jian-Bin Huang
    • Jia-Rui Feng
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Fifth Hospital of Wuhan, Wuhan, Hubei 430050, P.R. China, Department of Oncology, The Fifth Hospital of Wuhan, Wuhan, Hubei 430050, P.R. China
  • Pages: 1112-1118
    |
    Published online on: February 25, 2016
       https://doi.org/10.3892/ijmm.2016.2505
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Abstract

Colorectal cancer is one of the most common malignancies. Previous studies have reported that cortactin (CTTN) is often overexpressed in tumors and is associated with metastasis and poor prognosis of patients. The abnormal expression of microRNAs (miRNAs or miRs) is closely related to the development and progression of various types of cancer, including colorectal cancer. However, little is known about the miRNAs targeting cortactin. In the present study, prediction using biological software revealed that cortactin has binding sites for miR-542-3p. Transfection with miR-542-3p mimic demonstrated that miR‑542-3p reduced the expression of cortactin in colorectal cancer cells. Dual luciferase reporter assays further demonstrated that miR-542-3p regulated cortactin in a targeted manner and that miR-542-3p expression was significantly downregulated in colorectal cancer cells. A cell proliferation assay and Transwell migration assay were undertaken: we noted that miR‑542-3p inhibited the proliferation and invasion of colorectal cancer cells while promoting their apoptosis. By contrast, cortactin acted antagonistically. When co-transfected with miR-542-3p mimic and CTTN overexpression vector, the inhibitory effect of miR-542-3p was blocked. This indicates that miR-542-3p regulates CTTN in a targeted manner to modulate the growth and invasion of colorectal cancer cells. The present study thus provides new targets for the prevention and treatment of colorectal cancer.
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1 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Li M and Gu J: Changing patterns of colorectal cancer in China over a period of 20 years. World J Gastroenterol. 11:4685–4688. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Rothschild BL, Shim AH, Ammer AG, Kelley LC, Irby KB, Head JA, Chen L, Varella-Garcia M, Sacks PG, Frederick B, et al: Cortactin overexpression regulates actin-related protein 2/3 complex activity, motility, and invasion in carcinomas with chromosome 11q13 amplification. Cancer Res. 66:8017–8025. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Schuuring E: The involvement of the chromosome 11q13 region in human malignancies: cyclin D1 and EMS1 are two new candidate oncogenes - a review. Gene. 159:83–96. 1995. View Article : Google Scholar : PubMed/NCBI

5 

Lee YY, Yu CP, Lin CK, Nieh S, Hsu KF, Chiang H and Jin JS: Expression of survivin and cortactin in colorectal adenocarcinoma: association with clinicopathological parameters. Dis Markers. 26:9–18. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Luo ML, Shen XM, Zhang Y, Wei F, Xu X, Cai Y, Zhang X, Sun YT, Zhan QM, Wu M and Wang MR: Amplification and overexpression of CTTN (EMS1) contribute to the metastasis of esophageal squamous cell carcinoma by promoting cell migration and anoikis resistance. Cancer Res. 66:11690–11699. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Timpson P, Wilson AS, Lehrbach GM, Sutherland RL, Musgrove EA and Daly RJ: Aberrant expression of cortactin in head and neck squamous cell carcinoma cells is associated with enhanced cell proliferation and resistance to the epidermal growth factor receptor inhibitor gefitinib. Cancer Res. 67:9304–9314. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Buday L and Downward J: Roles of cortactin in tumor pathogenesis. Biochim Biophys Acta. 1775:263–273. 2007.PubMed/NCBI

9 

Chuma M, Sakamoto M, Yasuda J, Fujii G, Nakanishi K, Tsuchiya A, Ohta T, Asaka M and Hirohashi S: Overexpression of cortactin is involved in motility and metastasis of hepatocellular carcinoma. J Hepatol. 41:629–636. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Weaver AM: Cortactin in tumor invasiveness. Cancer Lett. 265:157–166. 2008. View Article : Google Scholar : PubMed/NCBI

11 

van Rossum AG, Moolenaar WH and Schuuring E: Cortactin affects cell migration by regulating intercellular adhesion and cell spreading. Exp Cell Res. 312:1658–1670. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Ayala I, Baldassarre M, Giacchetti G, Caldieri G, Tetè S, Luini A and Buccione R: Multiple regulatory inputs converge on cortactin to control invadopodia biogenesis and extracellular matrix degradation. J Cell Sci. 121:369–378. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Esquela-Kerscher A and Slack FJ: Oncomirs - microRNAs with a role in cancer. Nat Rev Cancer. 6:259–269. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Hashimoto Y, Akiyama Y and Yuasa Y: Multiple-to-multiple relationships between microRNAs and target genes in gastric cancer. PLoS One. 8:e625892013. View Article : Google Scholar : PubMed/NCBI

15 

Iwaya T, Yokobori T, Nishida N, Kogo R, Sudo T, Tanaka F, Shibata K, Sawada G, Takahashi Y, Ishibashi M, et al: Down-regulation of miR-144 is associated with colorectal cancer progression via activation of mTOR signaling pathway. Carcinogenesis. 33:2391–2397. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Zhang GJ, Xiao HX, Tian HP, Liu ZL, Xia SS and Zhou T: Upregulation of microRNA-155 promotes the migration and invasion of colorectal cancer cells through the regulation of claudin-1 expression. Int J Mol Med. 31:1375–1380. 2013.PubMed/NCBI

17 

Chiang Y, Song Y, Wang Z, Liu Z, Gao P, Liang J, Zhu J, Xing C and Xu H: microRNA-192, -194 and -215 are frequently down-regulated in colorectal cancer. Exp Ther Med. 3:560–566. 2012.PubMed/NCBI

18 

Goel A and Boland CR: Recent insights into the pathogenesis of colorectal cancer. Curr Opin Gastroenterol. 26:47–52. 2010. View Article : Google Scholar :

19 

Shibuya H, Iinuma H, Shimada R, Horiuchi A and Watanabe T: Clinicopathological and prognostic value of microRNA-21 and microRNA-155 in colorectal cancer. Oncology. 79:313–320. 2010. View Article : Google Scholar

20 

Althoff K, Lindner S, Odersky A, Mestdagh P, Beckers A, Karczewski S, Molenaar JJ, Bohrer A, Knauer S, Speleman F, et al: miR-542-3p exerts tumor suppressive functions in neuroblastoma by downregulating Survivin. Int J Cancer. 136:1308–1320. 2015. View Article : Google Scholar

21 

Yoon S, Choi YC, Lee S, Jeong Y, Yoon J and Baek K: Induction of growth arrest by miR-542-3p that targets survivin. FEBS Lett. 584:4048–4052. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Condeelis J and Segall JE: Intravital imaging of cell movement in tumours. Nat Rev Cancer. 3:921–930. 2003. View Article : Google Scholar

23 

Kedrin D, Wyckoff J, Sahai E, Condeelis J and Segall JE: Imaging tumor cell movement in vivo. Curr Protoc Cell Biol. Chapter 19: Unit 19.7. 2007. View Article : Google Scholar

24 

Kedrin D, van Rheenen J, Hernandez L, Condeelis J and Segall JE: Cell motility and cytoskeletal regulation in invasion and metastasis. J Mammary Gland Biol Neoplasia. 12:143–152. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Pollard TD, Blanchoin L and Mullins RD: Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu Rev Biophys Biomol Struct. 29:545–576. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Uruno T, Liu J, Zhang P, Fan YX, Egile C, Li R, Mueller SC and Zhan X: Activation of Arp2/3 complex-mediated actin polymerization by cortactin. Nat Cell Biol. 3:259–266. 2001. View Article : Google Scholar : PubMed/NCBI

27 

Hirakawa H, Shibata K and Nakayama T: Localization of cortactin is associated with colorectal cancer development. Int J Oncol. 35:1271–1276. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Schepeler T, Reinert JT, Ostenfeld MS, Christensen LL, Silahtaroglu, Dyrskjøt L, Wiuf C, Sørensen FJ, Kruhøffer M, Laurberg S, et al: Diagnostic and prognostic microRNAs in stage II colon cancer. Cancer Res. 68:6416–6424. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Slaby O, Svoboda M, Fabian P, Smerdova T, Knoflickova D, Bednarikova M, Nenutil R and Vyzula R: Altered expression of miR-21, miR-31, miR-143 and miR-145 is related to clinico-pathologic features of colorectal cancer. Oncology. 72:397–402. 2007. View Article : Google Scholar

30 

Zhang L, Liu T, Huang Y and Liu J: microRNA-182 inhibits the proliferation and invasion of human lung adenocarcinoma cells through its effect on human cortical actin-associated protein. Int J Mol Med. 28:381–388. 2011.PubMed/NCBI

31 

Hong CC, Chen PS, Chiou J, Chiu CF, Yang CY, Hsiao M, Chang YW, Yu YH, Hung MC, Hsu NW, et al: miR326 maturation is crucial for VEGF-C-driven cortactin expression and esophageal cancer progression. Cancer Res. 74:6280–6290. 2014. View Article : Google Scholar : PubMed/NCBI

32 

Wang WT, Zhao YN, Yan JX, Weng MY, Wang Y, Chen YQ and Hong SJ: Differentially expressed microRNAs in the serum of cervical squamous cell carcinoma patients before and after surgery. J Hematol Oncol. 7:62014. View Article : Google Scholar : PubMed/NCBI

33 

Wang Y, Huang JW, Castella M, Huntsman DG and Taniguchi T: p53 is positively regulated by miR-542-3p. Cancer Res. 74:3218–3227. 2014. View Article : Google Scholar : PubMed/NCBI

34 

He T, Qi F, Jia L, Wang S, Song N, Guo L, Fu Y and Luo Y: MicroRNA-542-3p inhibits tumour angiogenesis by targeting angiopoietin-2. J Pathol. 232:499–508. 2014. View Article : Google Scholar : PubMed/NCBI

35 

Shen X, Si Y, Yang Z, Wang Q, Yuan J and Zhang X: MicroRNA-542-3p suppresses cell growth of gastric cancer cells via targeting oncogene astrocyte-elevated gene-1. Med Oncol. 32:3612015. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Long H, Gao X, Lei C, Zhu B, Li L, Zeng C, Huang J and Feng J: miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin. Int J Mol Med 37: 1112-1118, 2016.
APA
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C. ... Feng, J. (2016). miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin. International Journal of Molecular Medicine, 37, 1112-1118. https://doi.org/10.3892/ijmm.2016.2505
MLA
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C., Huang, J., Feng, J."miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin". International Journal of Molecular Medicine 37.4 (2016): 1112-1118.
Chicago
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C., Huang, J., Feng, J."miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin". International Journal of Molecular Medicine 37, no. 4 (2016): 1112-1118. https://doi.org/10.3892/ijmm.2016.2505
Copy and paste a formatted citation
x
Spandidos Publications style
Long H, Gao X, Lei C, Zhu B, Li L, Zeng C, Huang J and Feng J: miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin. Int J Mol Med 37: 1112-1118, 2016.
APA
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C. ... Feng, J. (2016). miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin. International Journal of Molecular Medicine, 37, 1112-1118. https://doi.org/10.3892/ijmm.2016.2505
MLA
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C., Huang, J., Feng, J."miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin". International Journal of Molecular Medicine 37.4 (2016): 1112-1118.
Chicago
Long, H., Gao, X., Lei, C., Zhu, B., Li, L., Zeng, C., Huang, J., Feng, J."miR-542-3p inhibits the growth and invasion of colorectal cancer cells through targeted regulation of cortactin". International Journal of Molecular Medicine 37, no. 4 (2016): 1112-1118. https://doi.org/10.3892/ijmm.2016.2505
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