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International Journal of Molecular Medicine
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Article

Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice

  • Authors:
    • Chan-Mi Jeon
    • In-Sik Shin
    • Na-Rae Shin
    • Ju-Mi Hong
    • Ok-Kyoung Kwon
    • Jung-Hee Kim
    • Sei-Ryang Oh
    • Tran-The Bach
    • Do-Van Hai
    • Bui-Hong Quang
    • Sang-Ho Choi
    • Joongku Lee
    • Pyung-Keun Myung
    • Kyung-Seop Ahn
  • View Affiliations / Copyright

    Affiliations: Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongwon‑gu, Cheongju‑si, Chungcheongbuk‑do 28116, Republic of Korea, IEBR, Vietnam Academy of Science and Technology (VAST), Cay Giay, Ha Noi, Vietnam, International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong‑gu, Daejeon 34141, Republic of Korea, College of Pharmacy, Chungnam National University, Yuseong‑gu, Daejeon, Chungcheongnam‑do 34134, Republic of Korea
  • Pages: 1091-1098
    |
    Published online on: March 3, 2016
       https://doi.org/10.3892/ijmm.2016.2515
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Abstract

Clausena anisata (Willd.) Hook.f. ex Benth. (CA), which is widely used in traditional medicine, reportedly exerts antitumor, anti-inflammatory and other important therapeutic effects. The aim of the present study was to investigate the potential therapeutic effects of CA in a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and in LPS-stimulated RAW 264.7 cells. Male C57BL/6 mice were administered treatments for 3 days by oral gavage. On day 3, the mice were instilled intranasally with LPS or PBS followed 3 h later by oral CA (30 mg/kg) or vehicle administration. In vitro, CA decreased nitric oxide (NO) production and pro-inflammatory cytokines, such as interleukin (IL)-6 and prostaglandin E2 (PGE2), in LPS-stimulated RAW 264.7 cells. CA also reduced the expression of pro-inflammatory mediators, such as cyclooxygenase-2. In vivo, CA administration significantly reduced inflammatory cell numbers in the bronchoalveolar lavage fluid (BALF) and suppressed pro-inflammatory cytokine levels, including tumor necrosis factor-α (TNF-α), IL-6, and IL-1β, as well as reactive oxygen species production in the BALF. CA also effectively reduced airway inflammation in mouse lung tissue of an LPS-induced ALI mouse model, in addition to decreasing inhibitor κB (IκB) and nuclear factor-κB (NF-κB) p65 phosphorylation. Taken together, the findings demonstrated that CA inhibited inflammatory responses in a mouse model of LPS-induced ALI and in LPS-stimulated RAW 264.7 cells. Thus, CA is a potential candidate for development as an adjunctive treatment for inflammatory disorders, such as ALI.
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Copy and paste a formatted citation
Spandidos Publications style
Jeon C, Shin I, Shin N, Hong J, Kwon O, Kim J, Oh S, Bach T, Hai D, Quang B, Quang B, et al: Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice. Int J Mol Med 37: 1091-1098, 2016.
APA
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J. ... Ahn, K. (2016). Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice. International Journal of Molecular Medicine, 37, 1091-1098. https://doi.org/10.3892/ijmm.2016.2515
MLA
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J., Oh, S., Bach, T., Hai, D., Quang, B., Choi, S., Lee, J., Myung, P., Ahn, K."Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice". International Journal of Molecular Medicine 37.4 (2016): 1091-1098.
Chicago
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J., Oh, S., Bach, T., Hai, D., Quang, B., Choi, S., Lee, J., Myung, P., Ahn, K."Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice". International Journal of Molecular Medicine 37, no. 4 (2016): 1091-1098. https://doi.org/10.3892/ijmm.2016.2515
Copy and paste a formatted citation
x
Spandidos Publications style
Jeon C, Shin I, Shin N, Hong J, Kwon O, Kim J, Oh S, Bach T, Hai D, Quang B, Quang B, et al: Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice. Int J Mol Med 37: 1091-1098, 2016.
APA
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J. ... Ahn, K. (2016). Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice. International Journal of Molecular Medicine, 37, 1091-1098. https://doi.org/10.3892/ijmm.2016.2515
MLA
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J., Oh, S., Bach, T., Hai, D., Quang, B., Choi, S., Lee, J., Myung, P., Ahn, K."Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice". International Journal of Molecular Medicine 37.4 (2016): 1091-1098.
Chicago
Jeon, C., Shin, I., Shin, N., Hong, J., Kwon, O., Kim, J., Oh, S., Bach, T., Hai, D., Quang, B., Choi, S., Lee, J., Myung, P., Ahn, K."Clausena anisata-mediated protection against lipopolysaccharide-induced acute lung injury in mice". International Journal of Molecular Medicine 37, no. 4 (2016): 1091-1098. https://doi.org/10.3892/ijmm.2016.2515
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