5,7-Dihydroxy-6-geranylflavanone improves insulin sensitivity through PPARα/γ dual activation

  • Authors:
    • Woojung Lee
    • Goo Yoon
    • Min Cheol Kim
    • Hak Cheol Kwon
    • Gyu-Un Bae
    • Yong Kee Kim
    • Su-Nam Kim
  • View Affiliations

  • Published online on: March 17, 2016     https://doi.org/10.3892/ijmm.2016.2531
  • Pages: 1397-1404
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Abstract

In the present study, we demonstrate that 5,7-dihydroxy-6-geranylflavanone (DGF) isolated from Amorpha fruticosa (A. fruticosa) is a novel peroxisome proliferator-activated receptor (PPAR)α/γ dual agonist which may be used to improve insulin sensitivity. The extract from A. fruticosa increased the transcriptional activity of both PPARα and PPARγ which was, in part, driven by the active ingredient DGF. Treatment with DGF markedly enhanced the adipogenesis of 3T3-L1 preadipocytes, which was comparable to the effect of the PPARγ agonist, troglitazone. In addition, DGF was found to enhance fatty acid oxidation and glucose utilization through the dual activation of PPARα/γ. In addition treatment with DGF led to an improvement in insulin sensitivity, resulting in enhanced glucose uptake in muscle cells. The findings of our study data suggest that DGF may be used as potential therapeutic agent in the treatment of type 2 diabetes and related metabolic disorders by enhancing glucose and lipid metabolism.

Introduction

Type 2 diabetes mellitus (T2DM), which accounts for >90% of diabetes cases, is a common metabolic disease that is characterized by the resistance of target tissues to insulin stimulation (1). T2DM is usually associated with hyperglycemia, dyslipidemia, obesity, hypertension, fatty liver disease, atherosclerosis, certain types of cancer and cardiovascular diseases (2). The peptide hormone, insulin, lowers blood glucose levels by facilitating glucose uptake, mainly into skeletal muscle and fat tissue, and by inhibiting endogenous glucose production in the liver; insulin resistance occurs when a normal dose of the hormone is incapable of eliciting these metabolic responses (3). Multiple defects in intracellular events, including an impairment of the insulin signaling axis, disrupt glucose metabolism and reduce glycogen synthesis, thus contributing to insulin resistance (46).

Relieving insulin resistance has been considered a promising approach to the treatment of T2DM (7). Thiazolidinediones (TZDs) and fibrates are some of the most commonly used medications in the treatment of T2DM, hyperlipidemia and insulin resistance. These drugs bind to and activate peroxisome proliferator-activated receptors (PPARs), which results in the upregulation of several genes involved in glucose and lipid metabolism (8). The PPAR family, belonging to the nuclear hormone receptor family, consists of 3 isoforms, PPARα, PPARβ/δ and PPARγ (9). PPARγ is mainly expressed in adipose tissue and vascular tissue/macrophages (10), and is present in muscle and β cells at low levels (11); it affects genes involved in lipid synthesis and storage, and glucose homeostasis. It is the functioning receptor for TZDs, including rosiglitazone and pioglitazone (8). PPARα is predominantly expressed in the liver (12) and stimulates lipid consumption by enhancing the expression of fatty acid oxidation genes, resulting in the amelioration of hyperlipidemia (13). PPARα agonists, including fenofibrate have been shown to exert potent effects by reducing plasma triglyceride levels (14). The distinct metabolic effects of PPARα and PPARγ agonists on insulin sensitivity and lipid metabolism has encouraged the development of novel drugs which target both PPARα and PPARγ (1517). It has been proposed that the dual activation of PPARα and PPARγ may guarantee more desirable effects with limited adverse effects (1517). A number of PPARα/γ dual agonists have been identified and tested in obese and insulin-resistant individuals (18,19); however, the majority of these drugs have been shown to have unexpected side-effects, including heart failure, renal failure, urinary cancer and anemia (18,19). Therefore, the development of novel PPARα/γ dual agonists with few adverse effects is urgently required.

Amorpha fruticosa (A. fruticosa) (Leguminosae) has been applied traditionally in the treatment of hypertension, hematomas and contusions in China, Japan and Korea. Previous studies on this plant have reported a number of phenolic compounds, including stilbenes, flavonoids and rotenoids. In addition, their pharmacological activities, such as antimicrobial, antitumor and tumor necrosis factor (TNF)-α inhibitory activities have been investigated (20,21). In our previous studies, we demonstrated the therapeutic potential of amorphastilbol isolated from A. fruticosa in metabolic disorders through the dual activation of PPARα/γ in 3T3-L1 cell systems and animal models (22,23). In the present study, we identified another active ingredient, 5,7-dihydroxy-6-geranylflavanone (DGF; chemical structure shown in Fig. 1), isolated from A. fruticosa which promotes the dual activation of PPARα/γ and explored its pharmacological properties.

Materials and methods

Cell culture and chemicals

The CV-1 kindey cells, 3T3-L1 preadipocyte mouse embryonic fibroblasts and C2C12 mouse myoblasts were obtained from ATCC (Manassas, VA, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; HyClone Laboratories, Logan, UT, USA) and 1% penicillin/streptomycin (Invitrogen). The HepG2 hepatocellular carcinoma cells were purchased from ATCC and cultured in minimum essential medium with Earle's balanced salts (MEM/EBSS), supplemented with 10% FBS, 1% penicillin/streptomycin, 1X non-essential amino acid and 1 mM sodium pyruvate (both from Welgene, Daegu, Korea) at 37°C with 5% CO2 in air. Troglitazone (TRO; PPARγ agonist) and WY14643 (PPARα agonist) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and GW501516 (PPARδ agonist) was purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Extraction, isolation and identification of DGF from A. fruticosa

Fruits of A. fruticosa were collected in Gangwon Province, Korea in December 2012. The plant was authenticated and deposited at the KIST Gangneung Institute Herbarium, Gangneung, Korea. The fruit of A. fruticosa (300 g) was extracted 3 times with ethanol and evaporated under a vacuum at 40°C. The extract was reconstituted with 0.5 liter of water and re-extracted with 0.5 liter of ethyl acetate. A total of 5 pure compounds, amorphastilbol, DGF, 4,4′-dimethoxy-2′-hydroxychalcone, 4′,7-dimethoxy-5-hydroxyisoflavone and 12α-hydroxy-α-toxicarol, were isolated by preparative high-performance liquid chromatography (DeltaPrep 150 ml System, SunFire Silica 150×10 mm Column, Waters, MA, USA) and identified from this extract, the chemical structures of which are shown in Fig. 1.

Cell-based transactivation assay

The CV-1 cells were seeded in 24-well plates and cultured for 24 h prior to transfection. After 24 h, the medium was changed to 10% charcoal dextran-treated FBS-DMEM; 4 h following the change in medium, a DNA mixture containing a 3X multimerized PPRE-luciferase reporter plasmid (0.3 µg), pcDNA3-hPPAR (30 ng) and an internal control plasmid, pRL-SV-40 (5 ng), was transfected into the cells using the TransFast™ transfection reagent (Promega, Madison, WI, USA) (23). For RXRα reporter gene analysis, RXRα plasmid (30 ng) and RXRE-luciferase reporter plasmid (0.3 µg) were transfected into the CV-1 cells. Following 24 h of transfection, the cells were treated with 10 µM TRO or the indicated concentrations of plant extracts (1, 3, 10 and 30 µg/ml) or DGF (1, 3, 10 and 30 µM) and incubated for an additional 24 h. The luciferase activities of the cell lysates were measured using the Dual-Luciferase® reporter assay system according to the manufacturer's instructions (Promega). The relative luciferase activity was normalized to the corresponding Renilla luciferase activity to determine the transfection efficiency.

Adipocyte differentiation

Adipocyte differentiation was induced by treating the cells with differentiation medium (DM) containing hormonal coctail (10 µg/ml insulin, 1 µM dexamethasone and 0.5 mM 3-isobutyl-1-methylxanthine) with 10% FBS in DMEM for 48 h before the cells were switched to a maintenance medium with 10% FBS and 10 µg/ml insulin. After an additional 48 h, the medium was replaced with 10% FBS-DMEM. Thereafter, the medium was changed every 2 days. The test drugs were administered at the initiation of differentiation and at every medium change for 8 days. The lipid accumulation in the cells was detected by Oil Red O staining.

Ligand binding assay

The LanthaScreen™ TR-FRET PPAR competitive binding assay (Invitrogen) was performed according to the manufacturer's instructions (24). In brief, a mixture of 5 ng glutathione S-transferase fused to the PPAR ligand-binding domain (GST-PPAR-LBD), 5 nM Tb-GST antibody, 5 nM Fluormone Pan-PPAR Green, and serial dilutions of DGF (≤100 µM) was added to the wells of black 384-well plates to a total volume of 18 ml. Following 2 h of incubation in the dark, the FRET signal was measured by excitation at 340 nm and emission at 520 nm using the Infinite® M1000 microplate reader (Tecan Group Ltd., Männedorf, Switzerland).

Measurement of triglyceride content

Triglyceride contents were measured using the triglyceride determination kit (Sigma-Aldrich). Following treatment, the differentiated 3T3-L1 adipocytes were rinsed twice with PBS, scraped in 200 µl of saline solution (150 mM NaCl, 2 mM EDTA, 50 mM sodium phosphate, pH 7.4), sonicated to homogenize the cell suspension and the total triglyceride content was then measured. The total triglyceride content was determined by measuring the increase in absorbance at 540 nm using a microplate reader (PowerWave XS; BioTek Instruments Inc., Winooski, VT, USA) according to the manufacturer's instructions. The results were expressed as milligrams of triglyceride per milligram of cellular protein. The residual cell lysate was centrifuged at 12,000 rpm to remove the fatty layer, and was then assayed for the measurement of the protein content using a BCA protein assay kit (Sigma-Aldrich).

Measurement of glycerol-3-phosphate dehydrogenase (GPDH) activity

GPDH activity was measured using a GPDH) activity assay kit (MK426; Takara Bio Inc., Shiga, Japan). Following treatment, the differentiated 3T3-L1 adipocytes were rinsed twice with PBS, scraped into 200 µl enzyme extraction buffer (provided with the kit) and sonicated. GPDH activity was determined according to the decrease in NADH activity by measuring the decrease in the absorbance at 340 nm using a microplate reader, according to the manufacturer's instructions.

Gene expression analysis

Total RNA was isolated from the 3T3-L1 adipocytes or HepG2 cells transfected with pcDNA3-hPPARα plasmid using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. The RNA concentration of each sample was determined using a spectrophotometer (Nanodrop 2000; Thermo Fischer Scientific, Inc., Wilmington, DE, USA) at 260 nm; the integrity of each RNA sample was evaluated using the Agilent 2100 BioAnalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). cDNA synthesis was performed using 1 µg of total RNA in 20 µl with random primers and SuperScript II reverse transcriptase. Quantitative PCR (q-PCR) was performed with SYBR-Green fluorescent dye using the 7500 Real-Time PCR system (Applied Biosystems Life Technologies, Foster City, CA, USA). Data analyses were performed using the 7500 system SDS software version 1.3.1 (Applied Biosystems Life Technologies). In some cases, conventional PCR was performed using the GeneAmp PCR system 9700 (Applied Biosystems Life Technologies), and the integrity of the PCR product was verified by agarose gel electrophoresis and ethidium bromide staining. The sequences of the primers used in this study are listed in Table I.

Table I

Primer sequences used for RT-PCR and qPCR.

Table I

Primer sequences used for RT-PCR and qPCR.

Gene nameOligonucleotide sequence (5′→3′)Accession no.Gene expression analysis
mC/EBPαF: AGGTGCTGGAGTTGACCAGTNM_007678 qPCR/RT-PCR
R: CAGCCTAGAGATCCAGCGAC
mPPARγF: CAAGAATACCAAAGTGCGATCAANM_011146 qPCR
R: GAGCTGGGTCTTTTCAGAATAATAAG
mPPARγF: TTCGCTGATGCACTGCCTATNM_011146 RT-PCR
R: GCCAACAGCTTCTCCTTCTC
maP2F: AGTGAAAACTTCGATGATTACATGAANM_024406 qPCR
R: GCCTGCCACTTTCCTTGTG
maP2F: ATGTGTGATGCCTTTGTGGGANM_024406 RT-PCR
R: TGCCCTTTCATAAACTCTTGT
mGLUT4F: AGAGTCTAAAGCGCCTNM_009204 qPCR/RT-PCR
R: CCGAGACCAACGTGAA
mAdiponectinF: AGCCTGGAGAAGCCGCTTATNM_009605 qPCR/RT-PCR
R: TTGCAGTAGAACTTGCCAGTGC
mResistinF: TCAACTCCCTGTTTCCAAATGCNM_022984 qPCR/RT-PCR
R: TCTTCACGAATGTCCCACGA
mGAPDHF: TTGTTGCCATCAACGACCCCNM_008084 qPCR
R: GCCGTTGAATTTGCCGTGAG
mGAPDHF: ACCACAGTCCATGCCATCACNM_008084 RT-PCR
R: TCCACCACCCTGTTGCTGTA
hACO2F: GCGGACATGGCTACTCAAAGCNM_003500.1 RT-PCR
R: GCAGTGCACCTTAGCAGCCTG
hCPT1αF: AGACGGTGGAACAGAGGCTGAAGNM_001876.1 RT-PCR
R: TGAGACCAAACAAAGTGATGATGTCAG
hGAPDHF: ACCACAGTCCATGCCATCACNM_002046 RT-PCR
R: TCCACCACCCTGTTGCTGTA
Myotube formation and western blot analysis

The C2C12 myoblasts were cultured in DMEM until reaching 90% confluence. The cells were differentiated into myotubes with DMEM containing 2% horse serum for 4 days, and then incubated for 16 h in DMEM containing 2% BSA and 10% FBS in the absence or presence of 0.75 mM palmitate to induce insulin resistance. Subsequently, the DGF-treated cells were stimulated with 100 nM insulin for 10 min. Following stimulation, the cells were washed twice with PBS and harvested. For western blot analysis, antibodies to Akt (cs9272; 1:1,000), phospho-Ser473 Akt (cs4060; 1:1,000), insulin receptor (IR) β (cs3020; 1:1,000), phospho-Tyr1135/1136 insulin growth factor (IGF)-1Rβ/Tyr1150/1151 IRβ (cs3024; 1:1,000) were used (Cell Signaling Technology, Beverly, MA, USA).

2-NBDG glucose uptake assay

The myotubes, which were obtained from the above-mentioned procedures, were stimulated with 100 nM insulin for 1 h. Following insulin stimulation, the myotubes were incubated with 50 µM 2-NBDG (Invitrogen) for 15 min and then washed with PBS 3 times to remove free 2-NBDG. The fluorescence intensity of the cells containing 2-NBDG was measured using the Infinite M1000 microplate reader (Tecan Group Ltd.) with excitation at 485 nm and emission at 535 nm.

Statistical analysis

Data are expressed as the means ± SD. Differences between the mean values in the 2 groups were analyzed using one-way analysis of variance (ANOVA). A value of P<0.05 was considered to indicate a statistically significant difference.

Results

Dual activation of PPARα/γ transcriptional activity by A. fruticosa

To explore the pharmacological properties of A. fruticosa, we examined the effects of A. fruticosa on the transcriptional activation of PPARs. As shown in Fig. 2A and B, treatment with the extract of A. fruticosa led to an increase in PPARα- and PPARγ-reporter gene activities in a dose-dependent manner; however, it had a minimal effect on the transcriptional activation of PPARδ (Fig. 2C). In addition, treatment with A. fruticosa extract markedly enhanced the adipocyte differentiation of the 3T3-L1 cells, as shown by Oil Red O staining (Fig. 2D), which was accompanied by an increase in the expression of adipocyte marker genes, including CCAAT-enhancer-binding protein (C/EBP)α, PPARγ, adipocyte protein 2 (aP2), resistin, adiponectin, and glucose transporter type 4 (GLUT4) (Fig. 2E). These results strongly indicate the presence of a novel PPARα/γ agonist in the fruit extract of A. fruticosa.

DGF is a novel PPARα/γ dual activator

As the PPARα/γ-reporter gene activities and adipocyte differentiation were markedly enhanced by the extract of A. fruticosa, we wished to identify the active ingredients of A. fruticosa. Thus, we isolated and identified 5 compounds from A. fruticosa and evaluated their activities on PPARα/γ transcriptional activation. As shown in Table II, although amorphastilbol exhibited the most potent promoting effect on PPARα/γ transactivation, DGF also had a similar effect. The other compounds, 4,4′-dimethoxy-2′-hydroxychalcone, 4′,7-dimethoxy-5-hydroxyisoflavone and 12α-hydroxy-α-toxicarol, exerted weaker effects than those of amorphastilbol and DGF. Since the pharmacological properties of amorphastilbol have already been reported (22), in this study, we focused on the properties of DGF. As shown in Fig. 3A and B, treatment with DGF led to an increase in both PPARα- and PPARγ-reporter gene activities in a dose-dependent manner. However, DGF exerted a minimal effect on PPARδ transcription (Fig. 3C) and no detectable effect on retinoid X receptor (RXRα) was obsrved (Fig. 3D). The DGF binding affinities to PPARα and PPARγ were weaker than those of the positive controls, GW501516 and TRO (Fig. 3E), which is in agreement with the weak ability of DGF to activate the transcription of PPARα/γ in comparison to that of the positive control (Fig. 3A and B).

Table II

Effects of the compounds from A. fruticosa on PPARα/γ transcriptional activity.

Table II

Effects of the compounds from A. fruticosa on PPARα/γ transcriptional activity.

CompoundsTransactivation (fold)
PPARαPPARγ
DGF2.973.68
Amorphastilbol3.943.61
4,4′-Dimethoxy-2′-hydroxychalcone1.381.27
4′,7-Dimethoxy-5-hydroxyisoflavone1.652.38
12α-Hydroxy-α-toxicarol2.012.85
WY146434.71
TRO4.4

[i] Transcriptional activities of peroxisome proliferator-activated receptor (PPAR)-α and PPARγ in the presence of each compounds (30 µM) were analyzed, and the data are expressed as the fold increase in gene expression compared with the control. WY14643 (10 µM) and troglitazone (TRO) (10 µM) were used as positive controls of PPARα and PPARγ, respectively. DGF, 5,7-dihydroxy-6-geranyl flavanone; A. fruticosa, Amorpha fruticosa.

DGF upregulates specific genes involved in both adipocyte differentiation and fatty acid oxidation

To evaluate the potential effects of DGF on adipogenesis, 3T3-L1 preadipocytes were differentiated for 8 days in the presence of DGF. As shown in Fig. 4A and B, treatment with DGF induced a marked increase in lipid accumulation in the differentiated adipocytes, which was comparable to the effects of TRO. To further characterize the adipogenic potential of DGF, the cellular triglyceride content and GPDH enzyme activity were measured. The triglyceride content in the differentiated adipocytes was also increased by DGF treatment up to 1.5-fold (Fig. 4C); this observation was in accordance with the result that DGF induced lipid droplet accumulation in adipocytes, as shown in Fig. 4A. In addition, GPDH activity was significantly enhanced by DGF treatment (Fig. 4D). Taken together, these results strongly support the notion that DGF promotes the adipocyte differentiation of 3T3-L1 cells. As adipogenesis is governed by the increased expression of various transcription factors and adipocyte-specific genes (25), we examined the effects DGF on the expression of adipogenic transcription factors and marker genes in the differentiated adipocytes. Following the induction of adipocyte differentiation in the presence of DGF, the mRNA levels of C/EBPα, PPARγ, aP2, adiponectin and resistin were measured by qPCR. In the DGF-treated adipocytes, the mRNA levels of these genes were markedly increased compared to their levels in the absence of DGF (Fig. 4E). In addition, treatment with DGF induced an increase in the mRNA level of GLUT4, which is responsible for insulin-mediated glucose uptake (26), indicating that the insulin sensitivity of adipose tissue may be enhanced by DGF treatment. Subsequently, we examined the effects of DGF on fatty acid oxidation to confirm its PPARα agonistic effect. In the HepG2 cells transfected with the PPARα overexpression vector, the expression levels of two different PPARα target genes involved in β-oxidation, carnitine palmitoyltransferase 1α (CPT1α) and acyl-CoA oxidase 2 (ACO2), were increased by DGF treatment (Fig. 4F), supporting the hypothesis that DGF promotes fatty acid oxidation through the activation of PPARα.

DGF improves insulin sensitivity

To further ascertain the positive effects of DGF on insulin sensitivity, we first examined glucose uptake in C2C12 myotubes under normal conditions. As shown in Fig. 5A, glucose uptake in C2C12 myotubes in the absence or presence of insulin was significantly enhanced by DGF treatment under normal conditions. Correspondingly, treatment with DGF also enhanced the insulin-induced phosphorylation of IR and Akt under normal conditions (Fig. 5C). We then examined the effects of DGF on glucose uptake under palmitate-induced insulin-resistant conditions. DGF enhanced glucose uptake under insulin-resistant conditions as well (Fig. 5B). The effects of DGF on insulin sensitivity under insulin-resistant conditions were further confirmed by the elevated phosphorylation levels of these downstream proteins in insulin signaling (Fig. 5D). All these results suggest that the enhancement of insulin sensitivity by DGF is mediated by the insulin-induced activation of the IR-Akt signaling axis and enhanced glucose uptake.

Discussion

T2DM and related metabolic diseases pose a serious health concern in modern societies. We have recently reported that amorphastilbol isolated from A. fruticosa exerts beneficial effects on glucose and lipid metabolism by selectively activating both PPARα and PPARγ, thus ameliorating metabolic disorders without being associated with any severe adverse reactions that have been observed for other PPAR agonists, such as weight gain and hepatomegaly (22). In this study, we demonstrated that DGF, as another novel PPARα/γ dual agonist isolated from A. fruticosa, promoted adipogenesis through the upregulation of adipocyte specific marker gene expression, and improved insulin sensitivity. However, the binding affinities of DGF to both PPARα (>25 µM) and PPARγ (9.7 µM) were much weaker than those of amorphastilbol; the binding affinities of amorphastilbol to both PPARα and PPARγ were reported as 1.5 µM and 0.84 µM, respectively (22). Although its binding affinities to PPARα/γ were much weaker than those of amorphastilbol, DGF significantly increased their transcriptional activities, which resulted in the upregulation of adipocyte specific marker gene expression and the improvement of insulin sensitivity, indicating that DGF is one of the active ingredients of A. fruticosa with anti-diabetic properties.

It has been demonstrated that rosiglitazone, a full agonist of PPARγ, is associated with an increased risk of heart attacks, which potentially limits its appeal and further clinical use, in spite of its potent improvement of glucose metabolism (27). However, recently, FDA demonstrated that there is no direct evidence for the cardiovascular risk of rosiglitazone after reviewing the results of clinical trials (28). In addition, pioglitazone, another PPARγ agonist, is less frequently associated with increased cardiovascular risk (29). All these facts imply that PPARγ activity itself may not be directly associated with this cardiovascular risk. Therefore, the development of novel partial PPAR agonists that are structurally unrelated to TZDs, particularly rosiglitazone, remains appealing. From this point of view, it is noteworthy that amorphastilbol and DGF isolated from A. fruticosa are partial dual agonists of PPARα/γ and that their chemical structures are unique from those of TZDs. In addition, amorphastilbol and DGF do not affect hERG K+ channel activity, which is associated with cardiovascular toxicity (data not shown). Taken together, these data support, in part, the relative safe use of amorphastilbol and DGF with regard to cardiovascular risk.

Insulin resistance occurs when a normal level of insulin is incapable of eliciting its metabolic responses (3) and results from multiple intracellular defects, including an impairment of the insulin signaling axis (46). Our data demonstrated that DGF ameliorated insulin resistance by activating the insulin signaling axis, resulting in the enhancement of glucose uptake (Fig. 5A and B).

In conclusion, in this study, we demonstrate that DGF exerts beneficial effects on glucose and lipid metabolism, thus ameliorating metabolic disorders by selectively activating both PPARα and PPARγ. These results strongly suggest that DGF is one of active components of A. fruticosa with anti-diabetic and anti-metabolic properties. Therefore, our data provide strongly support the further development of A. fruticosa and its component DGF as anti-metabolic agents for the treatment of glucose and lipid abnormalities, as well as insulin resistance.

Acknowledgments

The present study was supported by grants from the Korea Institute of Science and Technology, Republic of Korea (no. 2Z04371) and the Sookmyung Women's University Research Grants (1-1403-0042).

References

1 

Rathmann W and Giani G: Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 27:2568–2569; author reply 2569. 2004. View Article : Google Scholar : PubMed/NCBI

2 

Olokoba AB, Obateru OA and Olokoba LB: Type 2 diabetes mellitus: a review of current trends. Oman Med J. 27:269–273. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Saltiel AR: New perspectives into the molecular pathogenesis and treatment of type 2 diabetes. Cell. 104:517–529. 2001. View Article : Google Scholar : PubMed/NCBI

4 

Bajaj M and Defronzo RA: Metabolic and molecular basis of insulin resistance. J Nucl Cardiol. 10:311–323. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Pendergrass M, Bertoldo A, Bonadonna R, Nucci G, Mandarino L, Cobelli C and Defronzo RA: Muscle glucose transport and phosphorylation in type 2 diabetic, obese nondiabetic, and genetically predisposed individuals. Am J Physiol Endocrinol Metab. 292:E92–E100. 2007. View Article : Google Scholar

6 

Cusi K, Maezono K, Osman A, Pendergrass M, Patti ME, Pratipanawatr T, DeFronzo RA, Kahn CR and Mandarino LJ: Insulin resistance differentially affects the PI 3-kinase- and MAP kinase-mediated signaling in human muscle. J Clin Invest. 105:311–320. 2000. View Article : Google Scholar : PubMed/NCBI

7 

Wilcox G: Insulin and insulin resistance. Clin Biochem Rev. 26:19–39. 2005.PubMed/NCBI

8 

Michalik L, Auwerx J, Berger JP, Chatterjee VK, Glass CK, Gonzalez FJ, Grimaldi PA, Kadowaki T, Lazar MA, O'Rahilly S, et al: International Union of Pharmacology. LXI. Peroxisome proliferator-activated receptors. Pharmacol Rev. 58:726–741. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Lee CH, Olson P and Evans RM: Minireview: lipid metabolism, metabolic diseases, and peroxisome proliferator-activated receptors. Endocrinology. 144:2201–2207. 2003. View Article : Google Scholar : PubMed/NCBI

10 

Vidal-Puig AJ, Considine RV, Jimenez-Liñan M, Werman A, Pories WJ, Caro JF and Flier JS: Peroxisome proliferator-activated receptor gene expression in human tissues. Effects of obesity, weight loss, and regulation by insulin and glucocorticoids. J Clin Invest. 99:2416–2422. 1997. View Article : Google Scholar : PubMed/NCBI

11 

Dubois M, Pattou F, Kerr-Conte J, Gmyr V, Vandewalle B, Desreumaux P, Auwerx J, Schoonjans K and Lefebvre J: Expression of peroxisome proliferator-activated receptor gamma (PPARgamma) in normal human pancreatic islet cells. Diabetologia. 43:1165–1169. 2000. View Article : Google Scholar : PubMed/NCBI

12 

Bünger M, Hooiveld GJ, Kersten S and Müller M: Exploration of PPAR functions by microarray technology - a paradigm for nutrigenomics. Biochim Biophys Acta. 1771:1046–1064. 2007. View Article : Google Scholar

13 

Superko HR: A review of combined hyperlipidaemia and its treatment with fenofibrate. J Int Med Res. 17:99–112. 1989.PubMed/NCBI

14 

Bajaj M, Suraamornkul S, Hardies LJ, Glass L, Musi N and DeFronzo RA: Effects of peroxisome proliferator-activated receptor (PPAR)-alpha and PPAR-gamma agonists on glucose and lipid metabolism in patients with type 2 diabetes mellitus. Diabetologia. 50:1723–1731. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Pickavance LC, Brand CL, Wassermann K and Wilding JP: The dual PPARalpha/gamma agonist, ragaglitazar, improves insulin sensitivity and metabolic profile equally with pioglitazone in diabetic and dietary obese ZDF rats. Br J Pharmacol. 144:308–316. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Reifel-Miller A, Otto K, Hawkins E, Barr R, Bensch WR, Bull C, Dana S, Klausing K, Martin JA, Rafaeloff-Phail R, et al: A peroxisome proliferator-activated receptor alpha/gamma dual agonist with a unique in vitro profile and potent glucose and lipid effects in rodent models of type 2 diabetes and dyslipidemia. Mol Endocrinol. 19:1593–1605. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Harrity T, Farrelly D, Tieman A, Chu C, Kunselman L, Gu L, Ponticiello R, Cap M, Qu F, Shao C, et al: Muraglitazar, a novel dual (alpha/gamma) peroxisome proliferator-activated receptor activator, improves diabetes and other metabolic abnormalities and preserves beta-cell function in db/db mice. Diabetes. 55:240–248. 2006. View Article : Google Scholar

18 

Mittra S, Sangle G, Tandon R, Sharma S, Roy S, Khanna V, Gupta A, Sattigeri J, Sharma L, Priyadarsiny P, et al: Increase in weight induced by muraglitazar, a dual PPARalpha-gamma agonist, in db/db mice: adipogenesis/or oedema? Br J Pharmacol. 150:480–487. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Adeghate E, Adem A, Hasan MY, Tekes K and Kalasz H: Medicinal Chemistry and Actions of Dual and Pan PPAR Modulators. Open Med Chem J. 5(Suppl 2): 93–98. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Dat NT, Lee JH, Lee K, Hong YS, Kim YH and Lee JJ: Phenolic constituents of Amorpha fruticosa that inhibit NF-kappaB activation and related gene expression. J Nat Prod. 71:1696–1700. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Cho JY, Kim PS, Park J, Yoo ES, Baik KU, Kim YK and Park MH: Inhibitor of tumor necrosis factor-alpha production in lipopolysaccharide-stimulated RAW264.7 cells from Amorpha fruticosa. J Ethnopharmacol. 70:127–133. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Lee W, Ham J, Kwon HC, Kim YK and Kim SN: Anti-diabetic effect of amorphastilbol through PPARα/γ dual activation in db/db mice. Biochem Biophys Res Commun. 432:73–79. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Lee W, Ham J, Kwon HC, Yoon G, Bae GU, Kim YK and Kim SN: Amorphastilbol exerts beneficial effects on glucose and lipid metabolism in mice consuming a high-fat-diet. Int J Mol Med. 36:527–533. 2015.PubMed/NCBI

24 

Kim T, Lee W, Jeong KH, Song JH, Park SH, Choi P, Kim SN, Lee S and Ham J: Total synthesis and dual PPARα/γ agonist effects of amorphastilbol and its synthetic derivatives. Bioorg Med Chem Lett. 22:4122–4126. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Soukas A, Socci ND, Saatkamp BD, Novelli S and Friedman JM: Distinct transcriptional profiles of adipogenesis in vivo and in vitro. J Biol Chem. 276:34167–34174. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Zierath JR, He L, Gumà A, Odegoard Wahlström E, Klip A and Wallberg-Henriksson H: Insulin action on glucose transport and plasma membrane GLUT4 content in skeletal muscle from patients with NIDDM. Diabetologia. 39:1180–1189. 1996. View Article : Google Scholar : PubMed/NCBI

27 

Nissen SE and Wolski K: Effect of rosiglitazone on the risk of myocardial infarction and death from cardiovascular causes. N Engl J Med. 356:2457–2471. 2007. View Article : Google Scholar : PubMed/NCBI

28 

U.S. Food and Drug Administration: Rosiglitazone-containing diabetes medicines: Drug Safety Communication - Removal of some prescribing and dispensing restrictions. FDA Drug Safety Communication. 2013.

29 

Lincoff AM, Wolski K, Nicholls SJ and Nissen SE: Pioglitazone and risk of cardiovascular events in patients with type 2 diabetes mellitus: a meta-analysis of randomized trials. JAMA. 298:1180–1188. 2007. View Article : Google Scholar : PubMed/NCBI

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May 2016
Volume 37 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Lee, W., Yoon, G., Kim, M.C., Kwon, H.C., Bae, G., Kim, Y.K., & Kim, S. (2016). 5,7-Dihydroxy-6-geranylflavanone improves insulin sensitivity through PPARα/γ dual activation. International Journal of Molecular Medicine, 37, 1397-1404. https://doi.org/10.3892/ijmm.2016.2531
MLA
Lee, W., Yoon, G., Kim, M. C., Kwon, H. C., Bae, G., Kim, Y. K., Kim, S."5,7-Dihydroxy-6-geranylflavanone improves insulin sensitivity through PPARα/γ dual activation". International Journal of Molecular Medicine 37.5 (2016): 1397-1404.
Chicago
Lee, W., Yoon, G., Kim, M. C., Kwon, H. C., Bae, G., Kim, Y. K., Kim, S."5,7-Dihydroxy-6-geranylflavanone improves insulin sensitivity through PPARα/γ dual activation". International Journal of Molecular Medicine 37, no. 5 (2016): 1397-1404. https://doi.org/10.3892/ijmm.2016.2531