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Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury

  • Authors:
    • Sokjoong Chung
    • Seungsoo Rho
    • Gijin Kim
    • So-Ra Kim
    • Kwang-Hyun Baek
    • Myungseo Kang
    • Helen Lew
  • View Affiliations / Copyright

    Affiliations: Department of Ophthalmology, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea, Department of Biomedical Science, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea, Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea
    Copyright: © Chung et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1170-1180
    |
    Published online on: March 17, 2016
       https://doi.org/10.3892/ijmm.2016.2532
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Abstract

The use of mesenchymal stem cells (MSCs) in cell therapy in regenerative medicine has great potential, particularly in the treatment of nerve injury. Umbilical cord blood (UCB) reportedly contains stem cells, which have been widely used as a hematopoietic source and may have therapeutic potential for neurological impairment. Although ongoing research is dedicated to the management of traumatic optic nerve injury using various measures, novel therapeutic strategies based on the complex underlying mechanisms responsible for optic nerve injury, such as inflammation and/or ischemia, are required. In the present study, a rat model of optic nerve crush (ONC) injury was established in order to examine the effects of transplanting human chorionic plate-derived MSCs (CP‑MSCs) isolated from the placenta, as well as human UCB mononuclear cells (CB-MNCs) on compressed rat optic nerves. Expression markers for inflammation, apoptosis, and optic nerve regeneration were analyzed, as well as the axon survival rate by direct counting. Increased axon survival rates were observed following the injection of CB‑MNCs at at 1 week post-transplantation compared with the controls. The levels of growth-associated protein-43 (GAP‑43) were increased after the injection of CB‑MNCs or CP‑MSCs compared with the controls, and the expression levels of hypoxia-inducible factor-1α (HIF-1α) were also significantly increased following the injection of CB-MNCs or CP-MSCs. ERM-like protein (ERMN) and SLIT-ROBO Rho GTPase activating protein 2 (SRGAP2) were found to be expressed in the optic nerves of the CP‑MSC-injected rats with ONC injury. The findings of our study suggest that the administration of CB‑MNCs or CP‑MSCs may promote axon survival through systemic concomitant mechanisms involving GAP‑43 and HIF‑1α. Taken together, these findings provide further understanding of the mechanisms repsonsible for optic nerve injury and may aid in the development of novel cell-based therapeutic strategies with future applications in regenerative medicine, particularly in the management of optic nerve disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Chung S, Rho S, Kim G, Kim S, Baek K, Kang M and Lew H: Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury. Int J Mol Med 37: 1170-1180, 2016.
APA
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., & Lew, H. (2016). Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury. International Journal of Molecular Medicine, 37, 1170-1180. https://doi.org/10.3892/ijmm.2016.2532
MLA
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., Lew, H."Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury". International Journal of Molecular Medicine 37.5 (2016): 1170-1180.
Chicago
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., Lew, H."Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury". International Journal of Molecular Medicine 37, no. 5 (2016): 1170-1180. https://doi.org/10.3892/ijmm.2016.2532
Copy and paste a formatted citation
x
Spandidos Publications style
Chung S, Rho S, Kim G, Kim S, Baek K, Kang M and Lew H: Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury. Int J Mol Med 37: 1170-1180, 2016.
APA
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., & Lew, H. (2016). Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury. International Journal of Molecular Medicine, 37, 1170-1180. https://doi.org/10.3892/ijmm.2016.2532
MLA
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., Lew, H."Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury". International Journal of Molecular Medicine 37.5 (2016): 1170-1180.
Chicago
Chung, S., Rho, S., Kim, G., Kim, S., Baek, K., Kang, M., Lew, H."Human umbilical cord blood mononuclear cells and chorionic plate-derived mesenchymal stem cells promote axon survival in a rat model of optic nerve crush injury". International Journal of Molecular Medicine 37, no. 5 (2016): 1170-1180. https://doi.org/10.3892/ijmm.2016.2532
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