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Article

Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein

  • Authors:
    • Xiaowu Wang
    • Wenpeng Dong
    • Binbin Yuan
    • Yongchao Yang
    • Dongpeng Yang
    • Xi Lin
    • Changfu Chen
    • Weida Zhang
  • View Affiliations / Copyright

    Affiliations: Center of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China
  • Pages: 1429-1436
    |
    Published online on: March 31, 2016
       https://doi.org/10.3892/ijmm.2016.2543
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Abstract

Heat stress (HS) is commonly used to refer to the heat load that an individual is subjected to due to either metabolic heat, or environmental factors, including high temperatures and high humidity levels. HS has been reported to affect and even damage the functioning of various organs; overexposure to high temperatures and high humidity may lead to accidental deaths. It has been suggested that the cardiovascular system is primarily targeted by exposure to HS conditions; the HS-induced dysfunction of cardiomyocytes, which is characterized by mitochondrial dysfunction, may result in the development of cardiovascular diseases. The excessive production of reactive oxygen species (ROS) also participates in mitochondrial dysfunction. However, effective methods for the prevention and treatment of mitochondrial and cardiovascular dysfunction induced by exposure to HS are lacking. In the present study, we hypothesized that vitamin E (VE), an antioxidant, is capable of preventing oxidative stress and mitochondrial injury in cardiomyocytes induced by exposure to HS. The results revealed that pre‑treatment with VE increased the expression of metallothionein (MT), which has previously been reported to confer cytoprotective effects, particularly on the cardiovascular system. Pre-treatment with VE restored mitochondrial function in cardiomyocytes under conditions of HS by increasing the expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α), nuclear respiratory factor 1 (NRF-1) and mitochondrial transcription factor A (TFAM), and by increasing adenosine triphosphate (ATP) levels. Furthermore, pre-treatment with VE decreased the production of ROS, which was induced by exposure to HS and thus exerted antioxidant effects. In addition, pre-treatment with VE attenuated oxidative stress induced by exposure to HS, as demonstrated by the increased levels of antioxidant enzymes [superoxide dismutase (SOD) and glutathione (GSH)], and by the decreased levels of markers of oxidative injury [malondialdehyde (MDA) and lactate dehydrogenase (LDH)]. Taken together, these findings suggest that pre-treatment with VE can prevent mitochondrial dysfunction and oxidative stress in cardiomyocytes induced by exposure to HS, by increasing the expression of MT.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Dong W, Yuan B, Yang Y, Yang D, Lin X, Chen C and Zhang W: Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein. Int J Mol Med 37: 1429-1436, 2016.
APA
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X. ... Zhang, W. (2016). Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein. International Journal of Molecular Medicine, 37, 1429-1436. https://doi.org/10.3892/ijmm.2016.2543
MLA
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X., Chen, C., Zhang, W."Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein". International Journal of Molecular Medicine 37.5 (2016): 1429-1436.
Chicago
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X., Chen, C., Zhang, W."Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein". International Journal of Molecular Medicine 37, no. 5 (2016): 1429-1436. https://doi.org/10.3892/ijmm.2016.2543
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Dong W, Yuan B, Yang Y, Yang D, Lin X, Chen C and Zhang W: Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein. Int J Mol Med 37: 1429-1436, 2016.
APA
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X. ... Zhang, W. (2016). Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein. International Journal of Molecular Medicine, 37, 1429-1436. https://doi.org/10.3892/ijmm.2016.2543
MLA
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X., Chen, C., Zhang, W."Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein". International Journal of Molecular Medicine 37.5 (2016): 1429-1436.
Chicago
Wang, X., Dong, W., Yuan, B., Yang, Y., Yang, D., Lin, X., Chen, C., Zhang, W."Vitamin E confers cytoprotective effects on cardiomyocytes under conditions of heat stress by increasing the expression of metallothionein". International Journal of Molecular Medicine 37, no. 5 (2016): 1429-1436. https://doi.org/10.3892/ijmm.2016.2543
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