Open Access

Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells

  • Authors:
    • Xiu-Yun Li
    • Lun Wu
    • Sheng-Wei Li
    • Wen-Bo Zhou
    • Meng-Yuan Wang
    • Guo-Qing Zuo
    • Chang-An Liu
    • Xiong Ding
  • View Affiliations

  • Published online on: April 12, 2016     https://doi.org/10.3892/ijmm.2016.2561
  • Pages: 1465-1474
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

FcγRIIIa (CD16) is a low-affinity Fc receptor of IgG. As the idio-binding receptor of IgG Fc, it plays an important role in the antibody-dependent cellular cytotoxicity of natural killer cells. The aim of the present study was to investigate the distribution of Kupffer cells (KCs) and the expression of their surface receptor FcγRIIIa in hepatocellular carcinoma. Furthermore, we also aimed to observe the functional mechanism of FcγRIIIa. Immunohistochemical analysis was employed to study KCs and FcγRIIIa. In order to explore the role of FcγRIIIa in the growth of cancer cells, KCs and H22 tumor cells were co-cultured in different serum. The mRNA expression levels of tumor necrosis factor (TNF)-α and FcγRIIIa were analyzed by RT-qPCR; the TNF-α and FcγRIIIa protein expression levels were examined by enzyme‑linked immunosorbent assay and western blot analysis, respectively. Our results showed that the number of Kuppfer cells in cancerous tissues (21.6±7.8) was lower than those in para-cancerous (68.8±9.1) tissues and adjacent normal hepatic tissues (62.0±1.9) (P<0.01); this decreased with the reduction in the differentiation degree of cancer (P<0.05). FcγRIIIa-positive cells were similar in morphology to KCs, and their distributive tendency was coincident (P<0.05). The increase in CD16a mRNA levels in the group treated with immune serum was 3.9-, 4.9- and 3.9-fold greater than that in the ordinary serum group at different time points, and CD16a protein expression also markedly increased (P<0.05). However, these effects were inhibited by the addition of anti-IgG Fc serum (P<0.05). The results of the present study suggested that FcγRIIIa resided in KCs, and it contributed to the inhibition of the growth of liver tumor cells.
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June-2016
Volume 37 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li X, Wu L, Li S, Zhou W, Wang M, Zuo G, Liu C and Ding X: Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells. Int J Mol Med 37: 1465-1474, 2016
APA
Li, X., Wu, L., Li, S., Zhou, W., Wang, M., Zuo, G. ... Ding, X. (2016). Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells. International Journal of Molecular Medicine, 37, 1465-1474. https://doi.org/10.3892/ijmm.2016.2561
MLA
Li, X., Wu, L., Li, S., Zhou, W., Wang, M., Zuo, G., Liu, C., Ding, X."Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells". International Journal of Molecular Medicine 37.6 (2016): 1465-1474.
Chicago
Li, X., Wu, L., Li, S., Zhou, W., Wang, M., Zuo, G., Liu, C., Ding, X."Effect of CD16a, the surface receptor of Kupffer cells, on the growth of hepatocellular carcinoma cells". International Journal of Molecular Medicine 37, no. 6 (2016): 1465-1474. https://doi.org/10.3892/ijmm.2016.2561