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Article Open Access

In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse

  • Authors:
    • Xiuhui Chen
    • Xianchao Kong
    • Dongzhe Liu
    • Peng Gao
    • Yanhua Zhang
    • Peiling Li
    • Meimei Liu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Surgery, Harbin Children's Hospital, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 95-104
    |
    Published online on: May 17, 2016
       https://doi.org/10.3892/ijmm.2016.2593
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Abstract

Pelvic organ prolapse (POP), is a common condition in parous women. Synthetic mesh was once considered to be the standard of care; however, the use of synthetic mesh is limited by severe complications, thus creating a need for novel approaches. The application of cell-based therapy with stem cells may be an ideal alternative, and specifically for vaginal prolapse. Abnormalities in vaginal smooth muscle (SM) play a role in the pathogenesis of POP, indicating that smooth muscle cells (SMCs) may be a potential therapeutic target. Endometrial regenerative cells (ERCs) are an easily accessible, readily available source of adult stem cells. In the present study, ERCs were obtained from human menstrual blood, and phase contrast microscopy and flow cytometry were performed to characterize the morphology and phenotype of the ERCs. SMC differentiation was induced by a transforming growth factor β1-based medium, and the induction conditions were optimized. We defined the SMC characteristics of the induced cells with regard to morphology and marker expression using transmission electron microscopy, western blot analysis, immunocytofluorescence and RT-PCR. Examining the expression of the components of the Smad pathway and phosphorylated Smad2 and Smad3 by western blot analysis, RT-PCR and quantitative PCR demonstrated that the ‘TGFBR2/ALK5/Smad2 and Smad3’ pathway is involved, and both Smad2 and Smad3 participated in SMC differentiation. Taken together, these findings indicate that ERCs may be a promising cell source for cellular therapy aimed at modulating SM function in the vagina wall and pelvic floor in order to treat POP.
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1 

Wu JM, Matthews CA, Conover MM, Pate V and Jonsson Funk M: Lifetime risk of stress urinary incontinence or pelvic organ prolapse surgery. Obstet Gynecol. 123:1201–1206. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Haylen BT, de Ridder D, Freeman RM, Swift SE, Berghmans B, Lee J, Monga A, Petri E, Rizk DE, Sand PK and Schaer GN: An International Urogynecological Association (IUGA)/International Continence Society (ICS) joint report on the terminology for female pelvic floor dysfunction. Neurourol Urodyn. 29:4–20. 2010.

3 

Hendrix SL, Clark A, Nygaard I, Aragaki A, Barnabei V and McTiernan A: Pelvic organ prolapse in the Women's Health Initiative: gravity and gravidity. Am J Obstet Gynecol. 186:1160–1166. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Olsen ALSV, Smith VJ, Bergstrom JO, Colling JC and Clark AL: Epidemiology of surgically managed pelvic organ prolapse and urinary incontinence. Obstet Gynecol. 89:501–506. 1997. View Article : Google Scholar : PubMed/NCBI

5 

Maher C, Feiner B, Baessler K and Schmid C: Surgical management of pelvic organ prolapse in women. Cochrane Database Syst Rev. 4:CD0040142013.PubMed/NCBI

6 

Baessler K, Hewson AD, Tunn R, Schuessler B and Maher CF: Severe mesh complications following intravaginal slingplasty. Obstet Gynecol. 106:713–716. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Nguyen JN, Jakus-Waldman SM, Walter AJ, White T and Menefee SA: Perioperative complications and reoperations after incontinence and prolapse surgeries using prosthetic implants. Obstet Gynecol. 119:539–546. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Liang R, Abramowitch S, Knight K, Palcsey S, Nolfi A, Feola A, Stein S and Moalli PA: Vaginal degeneration following implantation of synthetic mesh with increased stiffness. BJOG. 120:233–243. 2013. View Article : Google Scholar

9 

Ho MH, Heydarkhan S, Vernet D, Kovanecz I, Ferrini MG, Bhatia NN and Gonzalez-Cadavid NF: Stimulating vaginal repair in rats through skeletal muscle-derived stem cells seeded on small intestinal submucosal scaffolds. Obstet Gynecol. 114:300–309. 2009. View Article : Google Scholar : PubMed/NCBI

10 

Boennelycke M, Gras S and Lose G: Tissue engineering as a potential alternative or adjunct to surgical reconstruction in treating pelvic organ prolapse. Int Urogynecol J Pelvic Floor Dysfunct. 24:741–747. 2013. View Article : Google Scholar

11 

Northington GM, Basha M, Arya LA, Wein AJ and Chacko S: Contractile response of human anterior vaginal muscularis in women with and without pelvic organ prolapse. Reprod Sci. 18:296–303. 2011. View Article : Google Scholar : PubMed/NCBI

12 

Boreham MK, Wai CY, Miller RT, Schaffer JI and Word RA: Morphometric properties of the posterior vaginal wall in women with pelvic organ prolapse. Am J Obstet Gynecol. 187:1501–1508; discussion 1508–1509. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Inal HA, Kaplan PB, Usta U, Taştekin E, Aybatli A and Tokuc B: Neuromuscular morphometry of the vaginal wall in women with anterior vaginal wall prolapse. Neurourol Urodyn. 29:458–463. 2010.

14 

Bortolini MA, Shynlova O, Drutz HP, Castro RA, Girão MJ, Lye S and Alarab M: Expression of genes encoding smooth muscle contractile proteins in vaginal tissue of women with and without pelvic organ prolapse. Neurourol Urodyn. 31:109–114. 2012. View Article : Google Scholar

15 

Meijerink AM, van Rijssel RH and van der Linden PJ: Tissue composition of the vaginal wall in women with pelvic organ prolapse. Gynecol Obstet Invest. 75:21–27. 2013. View Article : Google Scholar

16 

Murphy MP, Wang H, Patel AN, Kambhampati S, Angle N, Chan K, Marleau AM, Pyszniak A, Carrier E, Ichim TE and Riordan NH: Allogeneic endometrial regenerative cells: an 'Off the shelf solution' for critical limb ischemia? J Transl Med. 6:452008. View Article : Google Scholar

17 

Zhong Z, Patel AN, Ichim TE, Riordan NH, Wang H, Min WP, Woods EJ, Reid M, Mansilla E, Marin GH, et al: Feasibility investigation of allogeneic endometrial regenerative cells. J Transl Med. 7:152009. View Article : Google Scholar : PubMed/NCBI

18 

Ichim TE, Alexandrescu DT, Solano F, Lara F, Campion RN, Paris E, Woods EJ, Murphy MP, Dasanu CA, Patel AN, et al: Mesenchymal stem cells as anti-inflammatories: implications for treatment of Duchenne muscular dystrophy. Cell Immunol. 260:75–82. 2010. View Article : Google Scholar

19 

Bockeria L, Bogin V, Bockeria O, Le T, Alekyan B, Woods EJ, Brown AA, Ichim TE and Patel AN: Endometrial regenerative cells for treatment of heart failure: a new stem cell enters the clinic. J Transl Med. 11:562013. View Article : Google Scholar : PubMed/NCBI

20 

Meng X, Ichim TE, Zhong J, Rogers A, Yin Z, Jackson J, Wang H, Ge W, Bogin V, Chan KW, et al: Endometrial regenerative cells: a novel stem cell population. J Transl Med. 5:572007. View Article : Google Scholar : PubMed/NCBI

21 

Patel AN, Park E, Kuzman M, Benetti F, Silva FJ and Allickson JG: Multipotent menstrual blood stromal stem cells: isolation, characterization, and differentiation. Cell Transplant. 17:303–311. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Hida N, Nishiyama N, Miyoshi S, Kira S, Segawa K, Uyama T, Mori T, Miyado K, Ikegami Y, Cui C, et al: Novel cardiac precursor-like cells from human menstrual blood-derived mesenchymal cells. Stem Cells. 26:1695–1704. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Rustandi RR, Loughney JW, Hamm M, Hamm C, Lancaster C, Mach A and Ha S: Qualitative and quantitative evaluation of Simon™, a new CE-based automated western blot system as applied to vaccine development. Electrophoresis. 23:2790–2797. 2012. View Article : Google Scholar

24 

Kohn EA, Yang YA, Du Z, Nagano Y, Van Schyndle CM, Herrmann MA, Heldman M, Chen JQ, Stuelten CH, Flanders KC and Wakefield LM: Biological responses to TGF-β in the mammary epithelium show a complex dependency on Smad3 gene dosage with important implications for tumor progression. Mol Cancer Res. 10:1389–1399. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Chen JQ, Heldman MR, Herrmann MA, Kedei N, Woo W, Blumberg PM and Goldsmith PK: Absolute quantitation of endogenous proteins with precision and accuracy using a capillary Western system. Anal Biochem. 442:97–103. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Bakhsheshian J, Hall MD, Robey RW, Herrmann MA, Chen JQ, Bates SE and Gottesman MM: Overlapping substrate and inhibitor specificity of human and murine ABCG2. Drug Metab Dispos. 41:1805–1812. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Klein BY, Tamir H, Hirschberg DL, Glickstein SB and Welch MG: Oxytocin modulates mTORC1 pathway in the gut. Biochem Biophys Res Commun. 432:466–471. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Xu D, Mane S and Sosic Z: Characterization of a biopharmaceutical protein and evaluation of its purification process using automated capillary western blot. Electrophoresis. 36:363–370. 2015. View Article : Google Scholar

29 

Satija NK, Singh VK, Verma YK, Gupta P, Sharma S, Afrin F, Sharma M, Sharma P, Tripathi RP and Gurudutta GU: Mesenchymal stem cell-based therapy: a new paradigm in regenerative medicine. J Cell Mol Med. 13:4385–4402. 2009. View Article : Google Scholar : PubMed/NCBI

30 

Mosna F, Sensebé L and Krampera M: Human bone marrow and adipose tissue mesenchymal stem cells: a user's guide. Stem Cells Dev. 19:1449–1470. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Nishimatsu H, Suzuki E, Kumano S, Nomiya A, Liu M, Kume H and Homma Y: Adrenomedullin mediates adipose tissue-derived stem cell-induced restoration of erectile function in diabetic rats. J Sex Med. 9:482–493. 2012. View Article : Google Scholar

32 

Ghionzoli M, Repele A, Sartiani L, Costanzi G, Parenti A, Spinelli V, David AL, Garriboli M, Totonelli G, Tian J, et al: Human amniotic fluid stem cell differentiation along smooth muscle lineage. FASEB J. 27:4853–4865. 2013. View Article : Google Scholar : PubMed/NCBI

33 

Sharma RR, Pollock K, Hubel A and McKenna D: Mesenchymal stem or stromal cells: a review of clinical applications and manufacturing practices. Transfusion. 54:1418–1437. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Kato K, Yoshimoto M, Kato K, Adachi S, Yamayoshi A, Arima T, Asanoma K, Kyo S, Nakahata T and Wake N: Characterization of side-population cells in human normal endometrium. Hum Reprod. 22:1214–1223. 2007. View Article : Google Scholar : PubMed/NCBI

35 

Schwab KE and Gargett CE: Co-expression of two perivascular cell markers isolates mesenchymal stem-like cells from human endometrium. Hum Reprod. 22:2903–2911. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Cervelló I, Gil-Sanchis C, Mas A, Delgado-Rosas F, Martínez-Conejero JA, Galán A, Martínez-Romero A, Martínez S, Navarro I, Ferro J, et al: Human endometrial side population cells exhibit genotypic, phenotypic and functional features of somatic stem cells. PLoS One. 5:e109642010. View Article : Google Scholar : PubMed/NCBI

37 

Cervelló I, Mas A, Gil-Sanchis C, Peris L, Faus A, Saunders PT, Critchley HO and Simón C: Reconstruction of endometrium from human endometrial side population cell lines. PLoS One. 6:e212212011. View Article : Google Scholar : PubMed/NCBI

38 

Ulrich D, Muralitharan R and Gargett CE: Toward the use of endometrial and menstrual blood mesenchymal stem cells for cell-based therapies. Expert Opin Biol Ther. 13:1387–1400. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Wang H, Jin P, Sabatino M, Ren J, Civini S, Bogin V, Ichim TE and Stroncek DF: Comparison of endometrial regenerative cells and bone marrow stromal cells. J Transl Med. 10:2072012. View Article : Google Scholar : PubMed/NCBI

40 

Lindskog H, Athley E, Larsson E, Lundin S, Hellström M and Lindahl P: New insights to vascular smooth muscle cell and pericyte differentiation of mouse embryonic stem cells in vitro. Arterioscler Thromb Vasc Biol. 26:1457–1464. 2006. View Article : Google Scholar : PubMed/NCBI

41 

Narita Y, Yamawaki A, Kagami H, Ueda M and Ueda Y: Effects of transforming growth factor-beta 1 and ascorbic acid on differentiation of human bone-marrow-derived mesenchymal stem cells into smooth muscle cell lineage. Cell Tissue Res. 333:449–459. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Pepe AE, Xiao Q, Zampetaki A, Zhang Z, Kobayashi A, Hu Y and Xu Q: Crucial role of nrf3 in smooth muscle cell differentiation from stem cells. Circ Res. 106:870–879. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Park JS, Chu JS, Tsou AD, Diop R, Tang Z, Wang A and Li S: The effect of matrix stiffness on the differentiation of mesenchymal stem cells in response to TGF-β. Biomaterials. 32:3921–3930. 2011. View Article : Google Scholar : PubMed/NCBI

44 

Valcourt U, Kowanetz M, Niimi H, Heldin CH and Moustakas A: TGF-beta and the Smad signaling pathway support transcriptomic reprogramming during epithelial-mesenchymal cell transition. Mol Biol Cell. 16:1987–2002. 2005. View Article : Google Scholar : PubMed/NCBI

45 

Santibañez JF, Quintanilla M and Bernabeu C: TGF-β/TGF-β receptor system and its role in physiological and pathological conditions. Clin Sci (Lond). 121:233–251. 2011. View Article : Google Scholar

46 

Tang Y, Yang X, Friesel RE, Vary CP and Liaw L: Mechanisms of TGF-β-induced differentiation in human vascular smooth muscle cells. J Vasc Res. 48:485–494. 2011. View Article : Google Scholar :

47 

Bobik A: Transforming growth factor-betas and vascular disorders. Arterioscler Thromb Vasc Biol. 26:1712–1720. 2006. View Article : Google Scholar : PubMed/NCBI

48 

Zhao Z, Yu H, Xiao F, Wang X, Yang S and Li S: Differentiation of adipose-derived stem cells promotes regeneration of smooth muscle for ureteral tissue engineering. J Surg Res. 178:55–62. 2012. View Article : Google Scholar : PubMed/NCBI

49 

Chen S and Lechleider RJ: Transforming growth factor-beta-induced differentiation of smooth muscle from a neural crest stem cell line. Circ Res. 94:1195–1202. 2004. View Article : Google Scholar : PubMed/NCBI

50 

Chou MT, Chang SN, Ke C, Chang HI, Sung ML, Kuo HC and Chen CN: The proliferation and differentiation of placental-derived multipotent cells into smooth muscle cells on fibrillar collagen. Biomaterials. 31:4367–4375. 2010. View Article : Google Scholar : PubMed/NCBI

51 

Wang D, Park JS, Chu JS, Krakowski A, Luo K, Chen DJ and Li S: Proteomic profiling of bone marrow mesenchymal stem cells upon transforming growth factor β1 stimulation. J Biol Chem. 279:43725–43734. 2004. View Article : Google Scholar : PubMed/NCBI

52 

Jia X, Glazener C, Mowatt G, MacLennan G, Bain C, Fraser C and Burr J: Efficacy and safety of using mesh or grafts in surgery for anterior and/or posterior vaginal wall prolapse: systematic review and meta-analysis. BJOG. 115:1350–1361. 2008. View Article : Google Scholar : PubMed/NCBI

53 

Roman S, Mangera A, Osman NI, Bullock AJ, Chapple CR and MacNeil S: Developing a tissue engineered repair material for treatment of stress urinary incontinence and pelvic organ prolapse-which cell source? Neurourol Urodyn. 33:531–537. 2014. View Article : Google Scholar

54 

Aboushwareb T, McKenzie P, Wezel F, Southgate J and Badlani G: Is tissue engineering and biomaterials the future for lower urinary tract dysfunction (LUTD)/pelvic organ prolapse (POP)? Neurourol Urodyn. 30:755–782. 2011. View Article : Google Scholar

55 

Hung MJ, Wen MC, Huang YT, Chen GD, Chou MM and Yang VC: Fascia tissue engineering with human adipose-derived stem cells in a murine model: implications for pelvic floor reconstruction. J Formos Med Assoc. 113:704–715. 2014. View Article : Google Scholar

56 

Su K, Edwards SL, Tan KS, White JF, Kandel S, Ramshaw JA, Gargett CE and Werkmeister JA: Induction of endometrial mesenchymal stem cells into tissue-forming cells suitable for fascial repair. Acta Biomater. 10:5012–5020. 2014. View Article : Google Scholar : PubMed/NCBI

57 

Smaldone MC and Chancellor MB: Muscle derived stem cell therapy for stress urinary incontinence. World J Urol. 26:327–332. 2008. View Article : Google Scholar : PubMed/NCBI

58 

Lin CS and Lue TF: Stem cell therapy for stress urinary incontinence: a critical review. Stem Cells Dev. 21:834–843. 2012. View Article : Google Scholar :

59 

Roche R, Festy F and Fritel X: Stem cells for stress urinary incontinence: the adipose promise. J Cell Mol Med. 14:135–142. 2010. View Article : Google Scholar

60 

Nikolavasky D, Stangel-Wójcikiewicz K, Stec M and Chancellor MB: Stem cell therapy: a future treatment of stress urinary incontinence. Semin Reprod Med. 29:61–70. 2011. View Article : Google Scholar : PubMed/NCBI

61 

Lin G, Wang G, Banie L, Ning H, Shindel AW, Fandel TM, Lue TF and Lin CS: Treatment of stress urinary incontinence with adipose tissue-derived stem cells. Cytotherapy. 12:88–95. 2010. View Article : Google Scholar :

62 

Shi LB, Cai HX, Chen LK, Wu Y, Zhu SA, Gong XN, Xia YX, Ouyang HW and Zou XH: Tissue engineered bulking agent with adipose-derived stem cells and silk fibroin microspheres for the treatment of intrinsic urethral sphincter deficiency. Biomaterials. 35:1519–1530. 2014. View Article : Google Scholar

63 

Dissaranan C, Cruz MA, Kiedrowski MJ, Balog BM, Gill BC, Penn MS, Goldman HB and Damaser MS: Rat mesenchymal stem cell secretome promotes elastogenesis and facilitates recovery from simulated childbirth injury. Cell Transplant. 23:1395–1406. 2014. View Article : Google Scholar

64 

Cruz M, Dissaranan C, Cotleur A, Kiedrowski M, Penn M and Damaser M: Pelvic organ distribution of mesenchymal stem cells injected intravenously after simulated childbirth injury in female rats. Obstet Gynecol Int. 2012:6129462012.

65 

Borlongan CV, Kaneko Y, Maki M, Yu SJ, Ali M, Allickson JG, Sanberg CD, Kuzmin-Nichols N and Sanberg PR: Menstrual blood cells display stem cell-like phenotypic markers and exert neuroprotection following transplantation in experimental stroke. Stem Cells Dev. 19:439–452. 2010. View Article : Google Scholar

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Chen X, Kong X, Liu D, Gao P, Zhang Y, Li P and Liu M: In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse. Int J Mol Med 38: 95-104, 2016.
APA
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., & Liu, M. (2016). In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse. International Journal of Molecular Medicine, 38, 95-104. https://doi.org/10.3892/ijmm.2016.2593
MLA
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., Liu, M."In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse". International Journal of Molecular Medicine 38.1 (2016): 95-104.
Chicago
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., Liu, M."In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse". International Journal of Molecular Medicine 38, no. 1 (2016): 95-104. https://doi.org/10.3892/ijmm.2016.2593
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Spandidos Publications style
Chen X, Kong X, Liu D, Gao P, Zhang Y, Li P and Liu M: In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse. Int J Mol Med 38: 95-104, 2016.
APA
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., & Liu, M. (2016). In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse. International Journal of Molecular Medicine, 38, 95-104. https://doi.org/10.3892/ijmm.2016.2593
MLA
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., Liu, M."In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse". International Journal of Molecular Medicine 38.1 (2016): 95-104.
Chicago
Chen, X., Kong, X., Liu, D., Gao, P., Zhang, Y., Li, P., Liu, M."In vitro differentiation of endometrial regenerative cells into smooth muscle cells: Α potential approach for the management of pelvic organ prolapse". International Journal of Molecular Medicine 38, no. 1 (2016): 95-104. https://doi.org/10.3892/ijmm.2016.2593
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