Biological effects of rutin on skin aging

Choi,Seong  Jin; Lee,Sung-Nae; Kim,Karam; Joo,Da  Hye; Shin,Shanghun; Lee,Jeongju; Lee,Hyun  Kyung; Kim,Jihyun; Kwon,Seung Bin; Kim,Min  Jung; Ahn,Kyu  Joong; An,In-Sook; An,Sungkwan; Cha,Hwa  Jun

doi:10.3892/ijmm.2016.2604

Biological effects of rutin on skin aging

Authors:
- Seong Jin Choi
- Sung-Nae Lee
- Karam Kim
- Da Hye Joo
- Shanghun Shin
- Jeongju Lee
- Hyun Kyung Lee
- Jihyun Kim
- Seung Bin Kwon
- Min Jung Kim
- Kyu Joong Ahn
- In-Sook An
- Sungkwan An
- Hwa Jun Cha
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Affiliations: Korea Institute for Skin and Clinical Sciences, Konkuk University, Seoul 143-701, Republic of Korea, GeneCellPharm Incorporated, Cheongju-si, Chungcheongbuk-do 361-951, Republic of Korea, Department of Dermatology, Konkuk University School of Medicine, Seoul 143-701, Republic of Korea
Published online on: May 24, 2016 https://doi.org/10.3892/ijmm.2016.2604
Pages: 357-363

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Abstract

Rutin, a quercetin glycoside is a member of the bioflavonoid family which is known to possess antioxidant properties. In the present study, we aimed to confirm the anti‑aging effects of rutin on human dermal fibroblasts (HDFs) and human skin. We examined the effects of rutin using a cell viability assay, senescence-associated-β-galactosidase assay, reverse transcription-quantitative polymerase chain reaction, and by measuring reactive oxygen species (ROS) scavenging activity in vitro. To examine the effects of rutin in vivo, rutin‑containing cream was applied to human skin. A double-blind clinical study was conducted in 40 subjects aged between 30-50 years and divided into control and experimental groups. The test material was applied for 4 weeks. After 2 and 4 weeks, dermal density, skin elasticity, the length and area of crow's feet, and number of under-eye wrinkles following the application of either the control or the rutin-containing cream were analyzed. Rutin increased the mRNA expression of collagen, type I, alpha 1 (COL1A1) and decreased the mRNA expression of matrix metallopeptidase 1 (MMP1) in HDFs. We verified that ROS scavenging activity was stimulated by rutin in a dose‑dependent manner and we identified that rutin exerted protective effects under conditions of oxidative stress. Furthermore, rutin increased skin elasticity and decreased the length, area and number of wrinkles. The consequences of human aging are primarily visible on the skin, such as increased wrinkling, sagging and decreased elasticity. Overall, this study demonstrated the biological effects of rutin on ROS-induced skin aging.

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