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Article Open Access

Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis

Retraction in: /10.3892/ijmm.2024.5476
  • Authors:
    • Dong-Yu Liang
    • Yan-Qiang Hou
    • Li-Jun Luo
    • Li Ao
  • View Affiliations / Copyright

    Affiliations: Department of Central Laboratory, Shanghai Jiading Central Hospital, Shanghai 201800, P.R. China, Department of Central Laboratory, Songjiang Hospital Affiliated to First People's Hospital, Shanghai Jiao Tong University, Shanghai 201600, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 131-138
    |
    Published online on: May 26, 2016
       https://doi.org/10.3892/ijmm.2016.2610
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Abstract

MicroRNAs (miRNAs or miRs) are small, non-coding RNA molecules that play significant roles in numerous diseases. However, there is limited information regarding the plasma expression of miRNAs in patients with primary biliary cirrhosis (PBC) as well as the potential role of miRNAs in the development of PBC. miRNA microarray analysis was performed using plasma obtaind from three patients with PBC and three healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to confirm the differential expression of miRNAs in the plasma and peripheral blood mononuclear cells (PBMCs) isolated from 20 patients with PBC, 20 patients with chronic hepatitis B (CHB) and 20 healthy controls. These miRNAs in PBMCs and plasma were validated by linear regression analyses. The T cell subset frequency was analyzed by flow cytometry. Correlations between altered miRNA expression and the frequency of the T cell subsets were determined by linear regression analyses. The co-expression of miRNAs and IL-17A was examined using fluorescence in situ hybridization (FISH) and immunohistochemistry. The microarray analysis identified sixteen miRNAs that were differentially expressed. Four miRNAs were validated by RT-qPCR. The expression pattern of miR-572 and miR-92a in the PBMCs correlated with the expression pattern in plasma. We also found that miR-92a expression closely correlated with the frequency of a subset of IL-17-producing T helper cells (Th17), and that miR-92a was co-expressed with IL-17A in patients with PBC. Taken together, these findings revealed that plasma from patients with PBC has a unique miRNA expression profile. Moreover, miR-92a may play an important role in the pathogenesis of PBC by regulating Th17 cell differentiation.
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Copy and paste a formatted citation
Spandidos Publications style
Liang D, Hou Y, Luo L and Ao L: Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476. Int J Mol Med 38: 131-138, 2016.
APA
Liang, D., Hou, Y., Luo, L., & Ao, L. (2016). Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476. International Journal of Molecular Medicine, 38, 131-138. https://doi.org/10.3892/ijmm.2016.2610
MLA
Liang, D., Hou, Y., Luo, L., Ao, L."Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476". International Journal of Molecular Medicine 38.1 (2016): 131-138.
Chicago
Liang, D., Hou, Y., Luo, L., Ao, L."Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476". International Journal of Molecular Medicine 38, no. 1 (2016): 131-138. https://doi.org/10.3892/ijmm.2016.2610
Copy and paste a formatted citation
x
Spandidos Publications style
Liang D, Hou Y, Luo L and Ao L: Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476. Int J Mol Med 38: 131-138, 2016.
APA
Liang, D., Hou, Y., Luo, L., & Ao, L. (2016). Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476. International Journal of Molecular Medicine, 38, 131-138. https://doi.org/10.3892/ijmm.2016.2610
MLA
Liang, D., Hou, Y., Luo, L., Ao, L."Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476". International Journal of Molecular Medicine 38.1 (2016): 131-138.
Chicago
Liang, D., Hou, Y., Luo, L., Ao, L."Altered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis Retraction in /10.3892/ijmm.2024.5476". International Journal of Molecular Medicine 38, no. 1 (2016): 131-138. https://doi.org/10.3892/ijmm.2016.2610
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