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Article Open Access

Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway

  • Authors:
    • Yanyan Jia
    • Jinyi Zhao
    • Meiyou Liu
    • Bingling Li
    • Ying Song
    • Yuwen Li
    • Aidong Wen
    • Lei Shi
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510010, P.R. China, Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
    Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 210-216
    |
    Published online on: May 31, 2016
       https://doi.org/10.3892/ijmm.2016.2616
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Abstract

Renal ischemia-reperfusion (I/R) injury is associated with high morbidity and mortality as there is currently no available effective therapeutic strategy with which to treat this injury. Thus, the aim of this study was to investigate the potential protective effects of brazilin, a major active component of the Chinese medicine Caesalpinia sappan L., against renal I/R injury in vitro and in vivo. Rats were subjected to removal of the right kidney and I/R injury to the left kidney (ischemia for 45 min followed by reperfusion for 24 h). Treatment with brazilin (30 mg/kg, administered intravenously at 30 min prior to ischemia) led to the reversal of I/R-induced changes in serum creatinine (Scr) and blood urea nitrogen (BUN) levels, and also attenuated the histopathological damage induced by I/R. Furthermore, TUNEL assay revealed that brazilin reduced cell necrosis, and significantly decreased the expression of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in renal tissue. Moreover, HK-2 cells were used in order to elucidate the mechanisms responsible for the protective effects of brazilin. The levels of phosphorylated IκBα and the nuclear translocation of nuclear factor-κB (NF-κB) were all evidently decreased by brazilin. These findings suggested that pre-treatment with brazilin protects against I/R-induced renal damage and suppresses the inflammatory response by inhibiting the activation of the NF-κB signaling pathway.
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View References

1 

Jang HR, Ko GJ, Wasowska BA and Rabb H: The interaction between ischemia-reperfusion and immune responses in the kidney. J Mol Med Berl. 87:859–864. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Sementilli A and Franco M: Renal acute cellular rejection: correlation between the immunophenotype and cytokine expression of the inflammatory cells in acute glomerulitis, arterial intimitis, and tubulointerstitial nephritis. Transplant Proc. 42:1671–1676. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Bagshaw SM, Bennett M, Haase M, Haase-Fielitz A, Egi M, Morimatsu H, D'amico G, Goldsmith D, Devarajan P and Bellomo R: Plasma and urine neutrophil gelatinase-associated lipocalin in septic versus non-septic acute kidney injury in critical illness. Intensive Care Med. 36:452–461. 2010. View Article : Google Scholar

4 

Friedericksen DV, Van der Merwe L, Hattingh TL, Nel DG and Moosa MR: Acute renal failure in the medical ICU still predictive of high mortality. S Afr Med J. 99:873–875. 2009.

5 

Parikh CR, Coca SG, Wang Y, Masoudi FA and Krumholz HM: Long-term prognosis of acute kidney injury after acute myocardial infarction. Arch Intern Med. 168:987–995. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Mansano AM, Vianna PT, Fabris VE, Silva LM, Braz LG and Castiglia YM: Prevention of renal ischemia/reperfusion injury in rats using acetylcysteine after anesthesia with isoflurane. Acta Cir Bras. 27:340–345. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Morgan CJ, Gill PJ, Lam S and Joffe AR: Peri-operative interventions, but not inflammatory mediators, increase risk of acute kidney injury after cardiac surgery: a prospective cohort study. Intensive Care Med. 39:934–941. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Shimizu S, Saito M, Kinoshita Y, Ohmasa F, Dimitriadis F, Shomori K, Hayashi A and Satoh K: Nicorandil ameliorates ischaemia-reperfusion injury in the rat kidney. Br J Pharmacol. 163:272–282. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Alsabbagh MM, Asmar A, Ejaz NI, Aiyer RK, Kambhampati G and Ejaz AA: Update on clinical trials for the prevention of acute kidney injury in patients undergoing cardiac surgery. Am J Surg. 206:86–95. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Di Nardo M, Ficarella A, Ricci Z, Luciano R, Stoppa F, Picardo S, Picca S, Muraca M and Cogo P: Impact of severe sepsis on serum and urinary biomarkers of acute kidney injury in critically ill children: An observational study. Blood Purif. 35:172–176. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Levy EM, Viscoli CM and Horwitz RI: The effect of acute renal failure on mortality. A cohort analysis. JAMA. 275:1489–1494. 1996. View Article : Google Scholar : PubMed/NCBI

12 

Santos WJ, Zanetta DM, Pires AC, Lobo SM, Lima EQ and Burdmann EA: Patients with ischaemic, mixed and nephrotoxic acute tubular necrosis in the intensive care unit - a homogeneous population? Crit Care. 10:R682006. View Article : Google Scholar

13 

Frantz S, Tillmanns J, Kuhlencordt PJ, Schmidt I, Adamek A, Dienesch C, Thum T, Gerondakis S, Ertl G and Bauersachs J: Tissue-specific effects of the nuclear factor kappaB subunit p50 on myocardial ischemia-reperfusion injury. Am J Pathol. 171:507–512. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Majid DS: Tumor necrosis factor-α and kidney function: Experimental findings in mice. Adv Exp Med Biol. 691:471–480. 2011. View Article : Google Scholar

15 

Shahid M, Francis J, Matrougui K and Majid DS: Involvement of tumor necrosis factor-alpha in natriuretic response to systemic infusion of nitric oxide synthase inhibitor in anesthetized mice. Am J Physiol Renal Physiol. 299:F217–F224. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Wang P, Wu P, Siegel MI, Egan RW and Billah MM: Interleukin (IL)-10 inhibits nuclear factor kappa B (NF kappa B) activation in human monocytes. IL-10 and IL-4 suppress cytokine synthesis by different mechanisms. J Biol Chem. 270:9558–9563. 1995. View Article : Google Scholar : PubMed/NCBI

17 

Bean AG, Freiberg RA, Andrade S, Menon S and Zlotnik A: Interleukin 10 protects mice against staphylococcal enterotoxin B-induced lethal shock. Infect Immun. 61:4937–4939. 1993.PubMed/NCBI

18 

Gérard C, Bruyns C, Marchant A, Abramowicz D, Vandenabeele P, Delvaux A, Fiers W, Goldman M and Velu T: Interleukin 10 reduces the release of tumor necrosis factor and prevents lethality in experimental endotoxemia. J Exp Med. 177:547–550. 1993. View Article : Google Scholar : PubMed/NCBI

19 

Fiorentino DF, Zlotnik A, Mosmann TR, Howard M and O'Garra A: IL-10 inhibits cytokine production by activated macrophages. J Immunol. 147:3815–3822. 1991.PubMed/NCBI

20 

Moon CK, Park KS, Kim SG, Won HS and Chung JH: Brazilin protects cultured rat hepatocytes from BrCCl3-induced toxicity. Drug Chem Toxicol. 15:81–91. 1992. View Article : Google Scholar : PubMed/NCBI

21 

Hu CM, Liu YH, Cheah KP, Li JS, Lam CS, Yu WY and Choy CS: Heme oxygenase-1 mediates the inhibitory actions of brazilin in RAW264.7 macrophages stimulated with lipopolysaccharide. J Ethnopharmacol. 121:79–85. 2009. View Article : Google Scholar

22 

Bae IK, Min HY, Han AR, Seo EK and Lee SK: Suppression of lipopolysaccharide-induced expression of inducible nitric oxide synthase by brazilin in RAW 264.7 macrophage cells. Eur J Pharmacol. 513:237–242. 2005. View Article : Google Scholar : PubMed/NCBI

23 

Kim B and Kim SH, Jeong SJ, Sohn EJ, Jung JH, Lee MH and Kim SH: Brazilin induces apoptosis and G2/M arrest via inactivation of histone deacetylase in multiple myeloma U266 cells. J Agric Food Chem. 60:9882–9889. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Guo J, Li L, Wu YJ, Yan Y, Xu XN, Wang SB, Yuan TY, Fang LH and Du GH: Inhibitory effects of Brazilin on the vascular smooth muscle cell proliferation and migration induced by PDGF-BB. Am J Chin Med. 41:1283–1296. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Chatterjee PK, Patel NS, Kvale EO, Cuzzocrea S, Brown PA, Stewart KN, Mota-Filipe H and Thiemermann C: Inhibition of inducible nitric oxide synthase reduces renal ischemia/reperfusion injury. Kidney Int. 61:862–871. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Chatterjee PK, Zacharowski K, Cuzzocrea S, Otto M and Thiemermann C: Inhibitors of poly (ADP-ribose) synthetase reduce renal ischemia-reperfusion injury in the anesthetized rat in vivo. FASEB J. 14:641–651. 2000.PubMed/NCBI

27 

Sharples EJ, Patel N, Brown P, Stewart K, Mota-Philipe H, Sheaff M, Kieswich J, Allen D, Harwood S, Raftery M, et al: Erythropoietin protects the kidney against the injury and dysfunction caused by ischemia-reperfusion. J Am Soc Nephrol. 15:2115–2124. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Shen J, Zhang HY, Lin H, Su H, Xing DM and Du LJ: Brazilein protects the brain against focal cerebral ischemia reperfusion injury correlating to inflammatory response suppression. Eur J Pharmacol. 558:88–95. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Bonventre JV and Weinberg JM: Recent advances in the pathophysiology of ischemic acute renal failure. J Am Soc Nephrol. 14:2199–2210. 2003. View Article : Google Scholar : PubMed/NCBI

30 

Wang LT, Chen BL, Wu CT, Huang KH, Chiang CK and Hwa Liu S: Protective role of AMP-activated protein kinase-evoked autophagy on an in vitro model of ischemia/reperfusion-induced renal tubular cell injury. PLoS One. 8:e798142013. View Article : Google Scholar : PubMed/NCBI

31 

Nichols TC: NF-kappaB and reperfusion injury. Drug News Perspect. 17:99–104. 2004. View Article : Google Scholar : PubMed/NCBI

32 

Lee HT, Kim M, Kim M, Kim N, Billings FT IV, D'Agati VD and Emala CW Sr: Isoflurane protects against renal ischemia and reperfusion injury and modulates leukocyte infiltration in mice. Am J Physiol Renal Physiol. 293:F713–F722. 2007. View Article : Google Scholar : PubMed/NCBI

33 

Kumar S, Allen DA, Kieswich JE, Patel NS, Harwood S, Mazzon E, Cuzzocrea S, Raftery MJ, Thiemermann C and Yaqoob MM: Dexamethasone ameliorates renal ischemia-reperfusion injury. J Am Soc Nephrol. 20:2412–2425. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Lee HT and Emala CW: Preconditioning and adenosine protect human proximal tubule cells in an in vitro model of ischemic injury. J Am Soc Nephrol. 13:2753–2761. 2002. View Article : Google Scholar : PubMed/NCBI

35 

Xie J and Guo Q: Apoptosis antagonizing transcription factor protects renal tubule cells against oxidative damage and apoptosis induced by ischemia-reperfusion. J Am Soc Nephrol. 17:3336–3346. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jia Y, Zhao J, Liu M, Li B, Song Y, Li Y, Wen A and Shi L: Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. Int J Mol Med 38: 210-216, 2016.
APA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y. ... Shi, L. (2016). Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. International Journal of Molecular Medicine, 38, 210-216. https://doi.org/10.3892/ijmm.2016.2616
MLA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38.1 (2016): 210-216.
Chicago
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38, no. 1 (2016): 210-216. https://doi.org/10.3892/ijmm.2016.2616
Copy and paste a formatted citation
x
Spandidos Publications style
Jia Y, Zhao J, Liu M, Li B, Song Y, Li Y, Wen A and Shi L: Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. Int J Mol Med 38: 210-216, 2016.
APA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y. ... Shi, L. (2016). Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway. International Journal of Molecular Medicine, 38, 210-216. https://doi.org/10.3892/ijmm.2016.2616
MLA
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38.1 (2016): 210-216.
Chicago
Jia, Y., Zhao, J., Liu, M., Li, B., Song, Y., Li, Y., Wen, A., Shi, L."Brazilin exerts protective effects against renal ischemia-reperfusion injury by inhibiting the NF-κB signaling pathway". International Journal of Molecular Medicine 38, no. 1 (2016): 210-216. https://doi.org/10.3892/ijmm.2016.2616
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