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Article

Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD

  • Authors:
    • Ying Chen
    • Peng Bai
    • Lili Liu
    • Junyan Han
    • Hui Zeng
    • Yongchang Sun
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, P.R. China, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China
  • Pages: 585-593
    |
    Published online on: June 8, 2016
       https://doi.org/10.3892/ijmm.2016.2629
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Abstract

Receptor activator of nuclear factor-κB ligand (RANKL)-expressing adaptive T cells contribute to bone damage in autoimmune arthritis, although their role in chronic obstructive pulmonary disease (COPD)-associated osteoporosis is unknown. In the present study, the functional expression of RANKL in CD4+/CD8+ T cells and Th17 cells, and the potential role of these cells in COPD-associated bone loss was investigated. A total of 36 non-smokers, 38 smokers with normal lung function and 57 patients with COPD were enrolled. Femoral and vertebral bone mineral density (BMD) was assessed by dual energy X-ray absorptiometry. RANKL expression in peripheral CD4+ and CD8+ T cells and Th17 cells was evaluated by flow cytometry. For in vitro experiments, CD4+ and CD8+ T cells from 17 non-smokers were evaluated for RANKL expression following dose-dependent culture with cigarette smoke extract (CSE) for 5 days. The frequencies of RANKL-positive CD4+ and CD8+ T cells were higher in the patients with COPD than in the non-smokers (P=0.001 and P=0.002, respectively). The proportion of CD4+ T cells positive for both RANKL and interleukin-17 (IL-17) was higher in the patients with COPD than in the non-smokers (P=0.010). However, the frequency of RANKL-expressing Th17 cells was similar among all groups (P=0.508). The frequency of RANKL+CD4+ T cells inversely correlated with BMD of the lumbar vertebrae (P=0.01, r=-0.229), and that of the femoral neck (P<0.001, r=-0.350). The results of our in vitro experiments revealed that CSE increased RANKL expression in CD4+ T cells only. The percentages of RANKL-positive CD4+ T cells and RANKL- and IL-17 double-positive CD4+ T cells were increased in the peripheral blood of patients with COPD, and the former were associated with BMD. These observations suggest that RANKL+CD4+ T cells may be mechanistically linked to diseases of the lung and bone in patients with COPD.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Bai P, Liu L, Han J, Zeng H and Sun Y: Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD. Int J Mol Med 38: 585-593, 2016.
APA
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., & Sun, Y. (2016). Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD. International Journal of Molecular Medicine, 38, 585-593. https://doi.org/10.3892/ijmm.2016.2629
MLA
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., Sun, Y."Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD". International Journal of Molecular Medicine 38.2 (2016): 585-593.
Chicago
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., Sun, Y."Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD". International Journal of Molecular Medicine 38, no. 2 (2016): 585-593. https://doi.org/10.3892/ijmm.2016.2629
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Bai P, Liu L, Han J, Zeng H and Sun Y: Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD. Int J Mol Med 38: 585-593, 2016.
APA
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., & Sun, Y. (2016). Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD. International Journal of Molecular Medicine, 38, 585-593. https://doi.org/10.3892/ijmm.2016.2629
MLA
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., Sun, Y."Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD". International Journal of Molecular Medicine 38.2 (2016): 585-593.
Chicago
Chen, Y., Bai, P., Liu, L., Han, J., Zeng, H., Sun, Y."Increased RANKL expression in peripheral T cells is associated with decreased bone mineral density in patients with COPD". International Journal of Molecular Medicine 38, no. 2 (2016): 585-593. https://doi.org/10.3892/ijmm.2016.2629
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