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Article Open Access

Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7

Retraction in: /10.3892/ijmm.2017.2860
  • Authors:
    • Jihui Duan
    • Zhiqin Tang
    • Hong Mu
    • Guojun Zhang
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory, Tianjin First Center Hospital, Tianjin 300192, P.R. China
    Copyright: © Duan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 399-406
    |
    Published online on: June 9, 2016
       https://doi.org/10.3892/ijmm.2016.2635
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Abstract

Bel1, a transactivator of the prototype foamy virus (PFV), plays pivotal roles in the replication of PFV. Previous studies have demonstrated that Bel1 bears a nuclear localization signal (NLS); however, its amino acid sequence remains unclear and the corresponding adaptor importins have not yet been identified. In this study, we inserted various fragments of Bel1 into an EGFP-GST fusion protein and investigated their subcellular localization by fluorescence microscopy. We found that the 215PRQKRPR221 fragment, which accords with the consensus sequence K(K/R)X(K/R) of the monopartite NLS, directed the nuclear translocation of Bel1. Point mutation experiments revealed that K218, R219 and R221 were essential for the nuclear localization of Bel1. The results of GST pull-down assay revealed that the Bel1 peptide 215-221, which bears the NLS, interacted with the nucleocytoplasmic transport receptors, karyopherin alpha 1 (importin alpha 5) (KPNA1), karyopherin alpha 6 (importin alpha 7) (KPNA6) and karyopherin alpha 7 (importin alpha 8) (KPNA7). Finally, in vitro nuclear import assays demonstrated that KPNA1, KPNA6 or KPNA7, along with other necessary nuclear factors, caused Bel1 to localize to the nucleus. Thus, the findings of our study indicate that KPNA1, KPNA6 and KPNA7 are involved in Bel1 nuclear distribution.
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Spandidos Publications style
Duan J, Tang Z, Mu H and Zhang G: Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860. Int J Mol Med 38: 399-406, 2016.
APA
Duan, J., Tang, Z., Mu, H., & Zhang, G. (2016). Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860. International Journal of Molecular Medicine, 38, 399-406. https://doi.org/10.3892/ijmm.2016.2635
MLA
Duan, J., Tang, Z., Mu, H., Zhang, G."Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860". International Journal of Molecular Medicine 38.2 (2016): 399-406.
Chicago
Duan, J., Tang, Z., Mu, H., Zhang, G."Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860". International Journal of Molecular Medicine 38, no. 2 (2016): 399-406. https://doi.org/10.3892/ijmm.2016.2635
Copy and paste a formatted citation
x
Spandidos Publications style
Duan J, Tang Z, Mu H and Zhang G: Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860. Int J Mol Med 38: 399-406, 2016.
APA
Duan, J., Tang, Z., Mu, H., & Zhang, G. (2016). Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860. International Journal of Molecular Medicine, 38, 399-406. https://doi.org/10.3892/ijmm.2016.2635
MLA
Duan, J., Tang, Z., Mu, H., Zhang, G."Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860". International Journal of Molecular Medicine 38.2 (2016): 399-406.
Chicago
Duan, J., Tang, Z., Mu, H., Zhang, G."Nuclear import of prototype foamy virus transactivator Bel1 is mediated by KPNA1, KPNA6 and KPNA7 Retraction in /10.3892/ijmm.2017.2860". International Journal of Molecular Medicine 38, no. 2 (2016): 399-406. https://doi.org/10.3892/ijmm.2016.2635
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