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Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2

  • Authors:
    • Yao Xing
    • Lijun Cui
    • Qianyan Kang
  • View Affiliations / Copyright

    Affiliations: Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China, Department of Ophthalmology, The First Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
    Copyright: © Xing et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 407-416
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    Published online on: June 16, 2016
       https://doi.org/10.3892/ijmm.2016.2644
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Abstract

The cytokine, transforming growth factor-β (TGF‑β), plays a key role in wound healing and tissue repair. Integrin‑linked kinase (ILK) is a downstream factor of the TGF-β signaling pathway. Research on ILK has mainly focused on its role in the invasion and metastasis of cancer cells. However, little has been reported on the effects of ILK in human Tenon's capsule fibroblasts (HTFs). In this study, we investigated the role of ILK in the proliferation and migration of HTFs exposed to TGF-β2. A lentiviral vector targeting ILK was screened from three candidates and the experimental result indicated that RNA interference can be used to inhibit ILK expression at both the mRNA and protein level. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to assess ILK mRNA expression. Cell proliferation was quantified by MTT assay and cell cycle progression was detected by flow cytometric analysis. Migration was measured by wound healing assay. It was observed that the silencing of ILK suppressed the TGF-β2-induced proliferation of HTFs and led to G1 phase cell cycle arrest and the significant downregulation of cyclin D1 expression. The migration ability of the HTFs decreased following the silencing of ILK, while the downregulation of α-smooth muscle actin expression and the upregulation of E-cadherin expression were observed. The findings of our study indicate that the silencing of ILK attenuates the abnormal proliferation and migration of HTFs induced by TGF-β2, which reveals the therapeutic potential of ILK inhibition in the prevention of scarring following glaucoma filtration surgery.
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Copy and paste a formatted citation
Spandidos Publications style
Xing Y, Cui L and Kang Q: Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2. Int J Mol Med 38: 407-416, 2016.
APA
Xing, Y., Cui, L., & Kang, Q. (2016). Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2. International Journal of Molecular Medicine, 38, 407-416. https://doi.org/10.3892/ijmm.2016.2644
MLA
Xing, Y., Cui, L., Kang, Q."Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2". International Journal of Molecular Medicine 38.2 (2016): 407-416.
Chicago
Xing, Y., Cui, L., Kang, Q."Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2". International Journal of Molecular Medicine 38, no. 2 (2016): 407-416. https://doi.org/10.3892/ijmm.2016.2644
Copy and paste a formatted citation
x
Spandidos Publications style
Xing Y, Cui L and Kang Q: Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2. Int J Mol Med 38: 407-416, 2016.
APA
Xing, Y., Cui, L., & Kang, Q. (2016). Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2. International Journal of Molecular Medicine, 38, 407-416. https://doi.org/10.3892/ijmm.2016.2644
MLA
Xing, Y., Cui, L., Kang, Q."Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2". International Journal of Molecular Medicine 38.2 (2016): 407-416.
Chicago
Xing, Y., Cui, L., Kang, Q."Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon's capsule fibroblasts induced by TGF-β2". International Journal of Molecular Medicine 38, no. 2 (2016): 407-416. https://doi.org/10.3892/ijmm.2016.2644
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