Transgenic overexpression of transient receptor potential vanilloid subtype 1 attenuates isoproterenol-induced myocardial fibrosis in mice

  • Authors:
    • Qiang Wang
    • Yunrong Zhang
    • De Li
    • Yan Zhang
    • Bing Tang
    • Gang Li
    • Yongjian Yang
    • Dachun Yang
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  • Published online on: June 16, 2016     https://doi.org/10.3892/ijmm.2016.2648
  • Pages: 601-609
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Abstract

Transient receptor potential vanilloid subtype 1 (TRPV1) is a non-selective cation channel with high permeability to Ca2+. Intracellular Ca2+ signaling is an essential regulator of endothelial nitric oxide (NO) synthase (eNOS) that plays a beneficial role in myocardial fibrosis. The aim of the present study was to determine the role of TRPV1 in isoproterenol-induced myocardial fibrosis. Transgenic mice overexpressing TRPV1 were generated on a C57BL/6J genetic background. An animal model of myocardial fibrosis was created by subcutaneously injecting the mice with isoproterenol. We found that the wild-type mice exhibited a significant increase in heart/body weight ratio, left ventricle/body weight ratio, left ventricular end-diastolic pressure (LVEDP), the cardiac fibrotic lesion area and collagen content, as well as a marked decrease in eNOS phosphorylation and NO/cyclic guanosine monophosphate (cGMP) levels at 2 weeks after the administration of isoproterenol (all p<0.01). However, these changes were significantly attenuated in the TRPV1 transgenic mice (p<0.05 or p<0.01). Moreover, the beneficial effects on myocardial fibrosis exerted by the overexpression of TRPV1 were attenuated by the administration of the eNOS inhibitor, Nω-nitro-L-arginine methyl ester (L-NAME) (all p<0.05). Similar anti-fibrotic effects were observed in in vitro experiments with primary cultured cardiac fibroblasts. The findings of our study suggest that TRPV1 overexpression attenuates isoproterenol‑induced myocardial fibrosis.
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August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang Q, Zhang Y, Li D, Zhang Y, Tang B, Li G, Yang Y and Yang D: Transgenic overexpression of transient receptor potential vanilloid subtype 1 attenuates isoproterenol-induced myocardial fibrosis in mice. Int J Mol Med 38: 601-609, 2016
APA
Wang, Q., Zhang, Y., Li, D., Zhang, Y., Tang, B., Li, G. ... Yang, D. (2016). Transgenic overexpression of transient receptor potential vanilloid subtype 1 attenuates isoproterenol-induced myocardial fibrosis in mice. International Journal of Molecular Medicine, 38, 601-609. https://doi.org/10.3892/ijmm.2016.2648
MLA
Wang, Q., Zhang, Y., Li, D., Zhang, Y., Tang, B., Li, G., Yang, Y., Yang, D."Transgenic overexpression of transient receptor potential vanilloid subtype 1 attenuates isoproterenol-induced myocardial fibrosis in mice". International Journal of Molecular Medicine 38.2 (2016): 601-609.
Chicago
Wang, Q., Zhang, Y., Li, D., Zhang, Y., Tang, B., Li, G., Yang, Y., Yang, D."Transgenic overexpression of transient receptor potential vanilloid subtype 1 attenuates isoproterenol-induced myocardial fibrosis in mice". International Journal of Molecular Medicine 38, no. 2 (2016): 601-609. https://doi.org/10.3892/ijmm.2016.2648