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Article

Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis

  • Authors:
    • Yi Liu
    • Zhen Lin
    • Jing Guo
    • Gaoli Xu
    • Ye Li
    • Taotao Xu
    • Huiqing Lv
    • Jianzhi Chen
    • Gang Wu
  • View Affiliations / Copyright

    Affiliations: School of Stomatology, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong 510630, P.R. China, The First Clinical Medical College, Zhejiang Chinese Medical University, Binjiang, Hangzhou, Zhejiang 310053, P.R. China, School of Pharmacy, Zhejiang Chinese Medical University, Binjiang, Hangzhou, Zhejiang 310053, P.R. China, Department of Oral Implantology and Prosthetic Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), VU University Amsterdam and University of Amsterdam, MOVE Research Institute, 1081 LA Amsterdam, Nord-Holland, The Netherlands
  • Pages: 537-544
    |
    Published online on: June 21, 2016
       https://doi.org/10.3892/ijmm.2016.2652
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Abstract

Notoginsenoside R1 (NGR1), one of the main effective components of Panax notoginseng, appears to be effective in promoting osteogenesis and treating osteoporosis. However, hitherto, whether NGR1 can directly promote osteoblastogenesis remains to be elucidated. In the present study, we hereby examined the effects of NGR1 on the osteoblastogenesis of a pre-osteoblast cell line (MC3T3-E1) in in vitro time-course and dose-dependent experiments. Its efficacy was evaluated by assessing cell viability (indicator of proliferation), alkaline phosphatase (ALP) activity (a marker of early osteoblastic differentiation), levels of osteocalcin (OCN; a marker of late osteoblastic differentiation), calcium deposition (a marker of final mineralization) and the expression of a series of osteoblastogenic marker genes (such as collagen Iα, Runx2, ALP and OCN) at different time points. When examining the proliferation of and ALP activity in the pre-osteoblasts, a bell-shaped dose-response pattern was observed when the cells were treated with various concentrations of NGR1, with a peak being observed at the concentration of 50 µg/ml. NGR1 markedly increased the expression of OCN at the concentration of 1,000 µg/ml in a dose‑dependent manner. Furthermore, treatment with 1,000 µg/ml NGR1 resulted in the highest mineralization by 4.3- and 5.9-fold on the 21st and 28th day, respectively compared with the control group (no treatment). On the whole, our findings indicate that NGR1 significantly promotes the osteoblastogenesis of pre-osteoblasts, which suggests that NGR1 has potential for use as a bone regeneration agent.
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View References

1 

Bi W, Gu Z, Zheng Y, Zhang X, Guo J and Wu G: Heterodimeric BMP-2/7 antagonizes the inhibition of all-trans retinoic acid and promotes the osteoblastogenesis. PLoS One. 8:e781982013. View Article : Google Scholar : PubMed/NCBI

2 

Zimmermann G and Moghaddam A: Allograft bone matrix versus synthetic bone graft substitutes. Injury. 42(Suppl 2): S16–S21. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Shadjou N and Hasanzadeh M: Silica-based mesoporous nano-biomaterials as promoter of bone regeneration process. J Biomed Mater Res A. 103:3703–3716. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Zheng Y, Wu G, Zhao J, Wang L, Sun P and Gu Z: rhBMP2/7 heterodimer: An osteoblastogenesis inducer of not higher potency but lower effective concentration compared with rhBMP2 and rhBMP7 homodimers. Tissue Eng Part A. 16:879–887. 2010. View Article : Google Scholar

5 

Govender S, Csimma C, Genant HK, Valentin-Opran A, Amit Y, Arbel R, Aro H, Atar D, Bishay M, Börner MG, et al: Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Joint Surg Am. 84-A:2123–2134. 2002.PubMed/NCBI

6 

Boden SD, Kang J, Sandhu H and Heller JG: Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans: a prospective, randomized clinical pilot trial: 2002 Volvo Award in clinical studies. Spine. 27:2662–2673. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Kaneko H, Arakawa T, Mano H, Kaneda T, Ogasawara A, Nakagawa M, Toyama Y, Yabe Y, Kumegawa M and Hakeda Y: Direct stimulation of osteoclastic bone resorption by bone morphogenetic protein (BMP)-2 and expression of BMP receptors in mature osteoclasts. Bone. 27:479–486. 2000. View Article : Google Scholar : PubMed/NCBI

8 

Uludag H, D'Augusta D, Palmer R, Timony G and Wozney J: Characterization of rhBMP-2 pharmacokinetics implanted with biomaterial carriers in the rat ectopic model. J Biomed Mater Res. 46:193–202. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Sokolsky-Papkov M, Agashi K, Olaye A, Shakesheff K and Domb AJ: Polymer carriers for drug delivery in tissue engineering. Adv Drug Deliv Rev. 59:187–206. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Carson JS and Bostrom MP: Synthetic bone scaffolds and fracture repair. Injury. 38(Suppl 1): S33–S37. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Kessler P, Thorwarth M, Bloch-Birkholz A, Nkenke E and Neukam FW: Harvesting of bone from the iliac crest - comparison of the anterior and posterior sites. Br J Oral Maxillofac Surg. 43:51–56. 2005. View Article : Google Scholar

12 

Yang X, Xiong X, Wang H and Wang J: Protective effects of Panax notoginseng saponins on cardiovascular diseases: a comprehensive overview of experimental studies. Evid Based Complement Alternat Med. 2014:2048402014. View Article : Google Scholar : PubMed/NCBI

13 

Qiang H, Zhang C, Shi ZB, Yang HQ and Wang KZ: Protective effects and mechanism of Panax notoginseng saponins on oxidative stress-induced damage and apoptosis of rabbit bone marrow stromal cells. Chin J Integr Med. 16:525–530. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Li XD, Liu ZY, Chang B, Liu DX, Chen B, Guo C, Wang YG, Xu JK, Huang DY and Du SX: Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways. Cell Physiol Biochem. 28:367–376. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Yin LM, Wang X, Qian XD, Lin XJ, Chen XH and Gao RL: Effects of Panax notoginseng saponins on proliferation and differentiation in NIH3T3 cells. Chin J Integr Med. 18:616–620. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Li XD, Chang B, Chen B, Liu ZY, Liu DX, Wang JS, Hou GQ, Huang DY and Du SX: Panax notoginseng saponins potentiate osteogenesis of bone marrow stromal cells by modulating gap junction intercellular communication activities. Cell Physiol Biochem. 26:1081–1092. 2010. View Article : Google Scholar

17 

Li XD, Wang JS, Chang B, Chen B, Guo C, Hou GQ, Huang DY and Du SX: Panax notoginseng saponins promotes proliferation and osteogenic differentiation of rat bone marrow stromal cells. J Ethnopharmacol. 134:268–274. 2011. View Article : Google Scholar

18 

Jia C, Xiong M, Wang P, Cui J, Du X, Yang Q, Wang W, Chen Y and Zhang T: Notoginsenoside R1 attenuates atherosclerotic lesions in ApoE deficient mouse model. PLoS One. 9:e998492014. View Article : Google Scholar : PubMed/NCBI

19 

Yu HS, Zhang LJ, Song XB, Liu YX, Zhang J, Cao M, Kang LP, Kang TG and Ma BP: Chemical constituents from processed rhizomes of Panax notoginseng. Zhongguo Zhong Yao Za Zhi. 38:3910–3917. 2013.In Chinese.

20 

Horii A, Wang X, Gelain F and Zhang S: Biological designer self-assembling peptide nanofiber scaffolds significantly enhance osteoblast proliferation, differentiation and 3-D migration. PLoS One. 2:e1902007. View Article : Google Scholar : PubMed/NCBI

21 

Fatokun AA, Stone TW and Smith RA: Hydrogen peroxide- induced oxidative stress in MC3T3-E1 cells: The effects of glutamate and protection by purines. Bone. 39:542–551. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Iwata T, Morotome Y, Tanabe T, Fukae M, Ishikawa I and Oida S: Noggin blocks osteoinductive activity of porcine enamel extracts. J Dent Res. 81:387–391. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Kuske B, Savkovic V and zur Nieden NI: Improved media compositions for the differentiation of embryonic stem cells into osteoblasts and chondrocytes. Methods Mol Biol. 690:195–215. 2011. View Article : Google Scholar

24 

Galindo M, Pratap J, Young DW, Hovhannisyan H, Im HJ, Choi JY, Lian JB, Stein JL, Stein GS and van Wijnen AJ: The bone-specific expression of Runx2 oscillates during the cell cycle to support a G1-related antiproliferative function in osteoblasts. J Biol Chem. 280:20274–20285. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Pratap J, Galindo M, Zaidi SK, Vradii D, Bhat BM, Robinson JA, Choi JY, Komori T, Stein JL, Lian JB, et al: Cell growth regulatory role of Runx2 during proliferative expansion of preosteoblasts. Cancer Res. 63:5357–5362. 2003.PubMed/NCBI

26 

Kang X, Li Z, Zhang WH, Zhou Q, Liu RH and Wang XJ: Study on effect of combination of Epimedii Folium and Ligustri Lucidi Fructus on osteoporosis rats induced by retinoic acid. Zhongguo Zhong Yao Za Zhi. 38:4124–4128. 2013.In Chinese.

27 

Wang X, Zhen L, Zhang G, Wong MS, Qin L and Yao X: Osteogenic effects of flavonoid aglycones from an osteoprotective fraction of Drynaria fortunei - an in vitro efficacy study. Phytomedicine. 18:868–872. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Ma HP, Ming LG, Ge BF, Zhai YK, Song P, Xian CJ and Chen KM: Icariin is more potent than genistein in promoting osteoblast differentiation and mineralization in vitro. J Cell Biochem. 112:916–923. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Tsuji-Naito K: Aldehydic components of cinnamon bark extract suppresses RANKL-induced osteoclastogenesis through NFATc1 downregulation. Bioorg Med Chem. 16:9176–9183. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Gong W and Li F: Quality evaluation on different specifications of Cervi Cornu Pantotrichum with its effect on ovariectomized osteoporosis model rats. Zhongguo Zhong Yao Za Zhi. 39:2326–2329. 2014.In Chinese. PubMed/NCBI

31 

Zhong L, Zhou XL, Liu YS, Wang YM, Ma F, Guo BL, Yan ZQ and Zhang QY: Estrogen receptor α mediates the effects of notoginsenoside R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes. Mol Med Rep. 12:119–126. 2015.PubMed/NCBI

32 

Shi X, Yu W, Yang T, Liu W, Zhao Y, Sun Y, Chai L, Gao Y, Dong Bin and Zhu L: Panax notoginseng saponins provide neuroprotection by regulating NgR1/RhoA/Rock2 pathway expression, in vitro and in vivo. J Ethnopharmacol. June 8–2016.Epub ahead of print. View Article : Google Scholar

33 

Wang P, Cui J, Du X, Yang Q, Jia C, Xiong M, Yu X, Li L, Wang W, Chen Y and Zhang T: Panax notoginseng saponins (PNS) inhibits breast cancer metastasis. J Ethnopharmacol. 154:663–671. 2014. View Article : Google Scholar : PubMed/NCBI

34 

Yang BR, Cheung KK, Zhou X, Xie RF, Cheng PP, Wu S, Zhou ZY, Tang JY, Hoi PM, Wang YH and Lee SM: Amelioration of acute myocardial infarction by saponins from flower buds of Panax notoginseng via pro-angiogenesis and anti-apoptosis. J Ethnopharmacol. 181:50–58. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Wang T, Guo R, Zhou G, Zhou X, Kou Z, Sui F, Li C, Tang L and Wang Z: Traditional uses, botany, phytochemistry, pharmacology and toxicology of Panax notoginseng (Burk.) F.H. Chen: A review. J Ethnopharmacol. May 3–2016.Epub ahead of print. View Article : Google Scholar

36 

Wang X, Jiang L, Wang X, Yin F, Li G, Feng X, Wang K and Sun S: Combination of autologous transplantation of G-CSF-mobilized peripheral blood mononuclear cells and Panax notoginseng saponins in the treatment of unreconstructable critical limb ischemia. Ann Vasc Surg. 28:1501–1512. 2014. View Article : Google Scholar : PubMed/NCBI

37 

Lee BA, Lee HS, Jung YS, Kim SW, Lee YW, Chang SH, Chung HJ, Kim OS and Kim YJ: The effects of a novel botanical agent on lipopolysaccharide-induced alveolar bone loss in rats. J Periodontol. 84:1221–1229. 2013. View Article : Google Scholar

38 

Chen B, Li XD, Liu DX, Wang H, Xie P, Liu ZY, Hou GQ, Chang B and Du SX: Canonical Wnt signaling is required for Panax notoginseng saponin-mediated attenuation of the RANKL/OPG ratio in bone marrow stromal cells during osteogenic differentiation. Phytomedicine. 19:1029–1034. 2012. View Article : Google Scholar : PubMed/NCBI

39 

Chen W, Dang Y and Zhu C: Simultaneous determination of three major bioactive saponins of Panax notoginseng using liquid chromatography-tandem mass spectrometry and a pharmacokinetic study. Chin Med. 5:122010. View Article : Google Scholar : PubMed/NCBI

40 

Sun Q, Meng QT, Jiang Y, Liu HM, Lei SQ, Su WT, Duan WN, Wu Y and Xia ZY and Xia ZY: Protective effect of ginsenoside Rb1 against intestinal ischemia-reperfusion induced acute renal injury in mice. PLoS One. 8:e808592013. View Article : Google Scholar : PubMed/NCBI

41 

Song ZJ, Tang LL and Li GL: The protective function and mechanism of notoginsenoside Rb1 against hypoxia hypercapnia-induced pulmonary vasoconstriction. Zhongguo Zhong Xi Yi Jie He Za Zhi. 32:1380–1384. 2012.In Chinese. PubMed/NCBI

42 

Shen Y, Li YQ, Li SP, Ma L, Ding LJ and Ji H: Alleviation of ovariectomy-induced osteoporosis in rats by Panax notoginseng saponins. J Nat Med. 64:336–345. 2010. View Article : Google Scholar : PubMed/NCBI

43 

Logan DC: Bacteriological standards in the investigation of enteric convalescents. BMJ. 1:222–223. 1942. View Article : Google Scholar : PubMed/NCBI

44 

Sasano Y, Li HC, Zhu JX, Imanaka-Yoshida K, Mizoguchi I and Kagayama M: Immunohistochemical localization of type I collagen, fibronectin and tenascin C during embryonic osteogenesis in the dentary of mandibles and tibias in rats. Histochem J. 32:591–598. 2000. View Article : Google Scholar

45 

Son JH, Cho YC, Sung IY, Kim IR, Park BS and Kim YD: Melatonin promotes osteoblast differentiation and mineralization of MC3T3-E1 cells under hypoxic conditions through activation of PKD/p38 pathways. J Pineal Res. 57:385–392. 2014. View Article : Google Scholar : PubMed/NCBI

46 

Okamura H, Yoshida K, Yang D and Haneji T: Protein phosphatase 2A Cα regulates osteoblast differentiation and the expressions of bone sialoprotein and osteocalcin via osterix transcription factor. J Cell Physiol. 228:1031–1037. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Lin Z, Guo J, Xu G, Li Y, Xu T, Lv H, Chen J and Wu G: Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis. Int J Mol Med 38: 537-544, 2016.
APA
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T. ... Wu, G. (2016). Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis. International Journal of Molecular Medicine, 38, 537-544. https://doi.org/10.3892/ijmm.2016.2652
MLA
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T., Lv, H., Chen, J., Wu, G."Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis". International Journal of Molecular Medicine 38.2 (2016): 537-544.
Chicago
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T., Lv, H., Chen, J., Wu, G."Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis". International Journal of Molecular Medicine 38, no. 2 (2016): 537-544. https://doi.org/10.3892/ijmm.2016.2652
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Lin Z, Guo J, Xu G, Li Y, Xu T, Lv H, Chen J and Wu G: Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis. Int J Mol Med 38: 537-544, 2016.
APA
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T. ... Wu, G. (2016). Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis. International Journal of Molecular Medicine, 38, 537-544. https://doi.org/10.3892/ijmm.2016.2652
MLA
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T., Lv, H., Chen, J., Wu, G."Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis". International Journal of Molecular Medicine 38.2 (2016): 537-544.
Chicago
Liu, Y., Lin, Z., Guo, J., Xu, G., Li, Y., Xu, T., Lv, H., Chen, J., Wu, G."Notoginsenoside R1 significantly promotes in vitro osteoblastogenesis". International Journal of Molecular Medicine 38, no. 2 (2016): 537-544. https://doi.org/10.3892/ijmm.2016.2652
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