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Article

miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α

  • Authors:
    • Zhijuan Fang
    • Peng Li
    • Wenhui Jia
    • Ting Jiang
    • Zhongyun Wang
    • Yang Xiang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu 210093, P.R. China, Department of Anesthesiology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 845-852
    |
    Published online on: July 1, 2016
       https://doi.org/10.3892/ijmm.2016.2659
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Abstract

MicroRNAs (miRNAs or miRs) are known to play a vital role in type 2 diabetes, and peroxisome proliferator-activated receptor-γ (PPARγ) coactivator-1α (PGC-1α) is involved in the pathogenesis of hepatic insulin resistance. However, the correlation, if any, between PGC-1α and miRNAs in the disease has not yet been determined. Thus, in the present study, we aimed to examine the correlation between PGC-1α and miRNAs in diabetes. For this purpose, we used primary hepatocytes isolated from C57BL/6 mice and ob/ob mice. First, we found an inverse correlation between miR‑696 and PGC-1α protein levels in vivo. Second, in vitro evidence demonstrated that PGC-1α expression was significantly decreased by infection with pre-miR‑696-LV, whereas infection with anti-miR‑696-LV increased the PGC-1α protein levels. Third, a luciferase reporter assay confirmed that miR‑696 directly recognizes a specific location within the 3'-untranslated region of PGC-1α transcripts. Furthermore, the biological consequences of miR‑696 targeting PGC-1α were determined by measuring the expression levels of the characteristic hepatic gluconeogenic enzyme, PEPCK, which is regulated by PGC-1α in the liver via the coactivation of transcription factors. Taken together, our findings demonstrate that miR‑696 plays an important role in the development of hepatic gluconeogenesis and insulin resistance through the inhibition of PGC-1α translation in the liver.
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Copy and paste a formatted citation
Spandidos Publications style
Fang Z, Li P, Jia W, Jiang T, Wang Z and Xiang Y: miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α. Int J Mol Med 38: 845-852, 2016.
APA
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., & Xiang, Y. (2016). miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α. International Journal of Molecular Medicine, 38, 845-852. https://doi.org/10.3892/ijmm.2016.2659
MLA
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., Xiang, Y."miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α". International Journal of Molecular Medicine 38.3 (2016): 845-852.
Chicago
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., Xiang, Y."miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α". International Journal of Molecular Medicine 38, no. 3 (2016): 845-852. https://doi.org/10.3892/ijmm.2016.2659
Copy and paste a formatted citation
x
Spandidos Publications style
Fang Z, Li P, Jia W, Jiang T, Wang Z and Xiang Y: miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α. Int J Mol Med 38: 845-852, 2016.
APA
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., & Xiang, Y. (2016). miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α. International Journal of Molecular Medicine, 38, 845-852. https://doi.org/10.3892/ijmm.2016.2659
MLA
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., Xiang, Y."miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α". International Journal of Molecular Medicine 38.3 (2016): 845-852.
Chicago
Fang, Z., Li, P., Jia, W., Jiang, T., Wang, Z., Xiang, Y."miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α". International Journal of Molecular Medicine 38, no. 3 (2016): 845-852. https://doi.org/10.3892/ijmm.2016.2659
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