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Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis

  • Authors:
    • Xiaohui Zhang
    • Xiaohua Zhang
    • Yiqun Xiong
    • Chaoying Xu
    • Xinliang Liu
    • Jian Lin
    • Guiping Mu
    • Shaogang Xu
    • Wenhe Liu
  • View Affiliations / Copyright

    Affiliations: Central Laboratory, Shenzhen Hospital Affiliated to Guangzhou University of Traditional Chinese Medicine, Shenzhen, Guangdong 518033, P.R. China, Cardiac Signaling Center, Medical University of South Carolina, Charleston, SC 29425, USA
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 758-766
    |
    Published online on: July 5, 2016
       https://doi.org/10.3892/ijmm.2016.2664
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Abstract

The sarcolemmal ATP-sensitive K+ (sarcKATP) channel plays a cardioprotective role during stress. However, the role of the sarcKATP channel in the apoptosis of cardiomyocytes and association with mitochondrial calcium remains unclear. For this purpose, we developed a model of LPS-induced sepsis in neonatal rat cardiomyocytes (NRCs). The TUNEL assay was performed in order to detect the apoptosis of cardiac myocytes and the MTT assay was performed to determine cellular viability. Exposure to LPS significantly decreased the viability of the NRCs as well as the expression of Bcl-2, whereas it enhanced the activity and expression of the apoptosis-related proteins caspase-3 and Bax, respectively. The sarcKATP channel blocker, HMR-1098, increased the apoptosis of NRCs, whereas the specific sarcKATP channel opener, P-1075, reduced the apoptosis of NRCs. The mitochondrial calcium uniporter inhibitor ruthenium red (RR) partially inhibited the pro-apoptotic effect of HMR-1098. In order to confirm the role of the sarcKATP channel, we constructed a recombinant adenovirus vector carrying the sarcKATP channel mutant subunit Kir6.2AAA to inhibit the channel activity. Kir6.2AAA adenovirus infection in NRCs significantly aggravated the apoptosis of myocytes induced by LPS. Elucidating the regulatory mechanisms of the sarcKATP channel in apoptosis may facilitate the development of novel therapeutic targets and strategies for the management of sepsis and cardiac dysfunction.
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1 

Merx MW and Weber C: Sepsis and the heart. Circulation. 116:793–802. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Zanotti-Cavazzoni SL and Hollenberg SM: Cardiac dysfunction in severe sepsis and septic shock. Curr Opin Crit Care. 15:392–397. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Lancel S, Joulin O, Favory R, Goossens JF, Kluza J, Chopin C, Formstecher P, Marchetti P and Neviere R: Ventricular myocyte caspases are directly responsible for endotoxin-induced cardiac dysfunction. Circulation. 111:2596–2604. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Suzuki J, Bayna E, Dalle Molle E and Lew WY: Nicotine inhibits cardiac apoptosis induced by lipopolysaccharide in rats. J Am Coll Cardiol. 41:482–488. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Noma A: ATP-regulated K+ channels in cardiac muscle. Nature. 305:147–148. 1983. View Article : Google Scholar : PubMed/NCBI

6 

Zhou F, Yao HH, Wu JY, Ding JH, Sun T and Hu G: Opening of microglial K(ATP) channels inhibits rotenone-induced neuroinflammation. J Cell Mol Med. 12:1559–1570. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Liu H, Zhang HY, Zhu X, Shao Z and Yao Z: Preconditioning blocks cardiocyte apoptosis: role of K(ATP) channels and PKC-epsilon. Am J Physiol Heart Circ Physiol. 282:H1380–H1386. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Kane GC, Liu XK, Yamada S, Olson TM and Terzic A: Cardiac KATP channels in health and disease. J Mol Cell Cardiol. 38:937–943. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Buckley JF, Singer M and Clapp LH: Role of KATP channels in sepsis. Cardiovasc Res. 72:220–230. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Yang ZW, Chen JK, Ni M, Zhao T, Deng YP, Tao X, Jiang GJ and Shen FM: Role of Kir6.2 subunits of ATP-sensitive potassium channels in endotoxemia-induced cardiac dysfunction. Cardiovasc Diabetol. 12:752013. View Article : Google Scholar : PubMed/NCBI

11 

Kuo JH, Chen SJ, Shih CC, Lue WM and Wu CC: Abnormal activation of potassium channels in aortic smooth muscle of rats with peritonitis-induced septic shock. Shock. 32:74–79. 2009. View Article : Google Scholar

12 

Shi W, Cui N, Wu Z, Yang Y, Zhang S, Gai H, Zhu D and Jiang C: Lipopolysaccharides up-regulate Kir6.1/SUR2B channel expression and enhance vascular KATP channel activity via NF-kappaB-dependent signaling. J Biol Chem. 285:3021–3029. 2010. View Article : Google Scholar

13 

Charalambous BM, Stephens RC, Feavers IM and Montgomery HE: Role of bacterial endotoxin in chronic heart failure: the gut of the matter. Shock. 28:15–23. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Shen M, Wu RX, Zhao L, Li J, Guo HT, Fan R, Cui Y, Wang YM, Yue SQ and Pei JM: Resveratrol attenuates ischemia/reperfusion injury in neonatal cardiomyocytes and its underlying mechanism. PLoS One. 7:e512232012. View Article : Google Scholar

15 

Nichols CG: KATP channels as molecular sensors of cellular metabolism. Nature. 440:470–476. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Sellitto AD, Al-Dadah AS, Schuessler RB, Nichols CG and Lawton JS: An open sarcolemmal adenosine triphosphate-sensitive potassium channel is necessary for detrimental myocyte swelling secondary to stress. Circulation. 124(Suppl 11): S70–S74. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Suzuki M, Sasaki N, Miki T, Sakamoto N, Ohmoto-Sekine Y, Tamagawa M, Seino S, Marbán E and Nakaya H: Role of sarcolemmal K(ATP) channels in cardioprotection against ischemia/reperfusion injury in mice. J Clin Invest. 109:509–516. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Zingman LV, Hodgson DM, Bast PH, Kane GC, Perez-Terzic C, Gumina RJ, Pucar D, Bienengraeber M, Dzeja PP, Miki T, et al: Kir6.2 is required for adaptation to stress. Proc Natl Acad Sci USA. 99:13278–13283. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Gumina RJ, Pucar D, Bast P, Hodgson DM, Kurtz CE, Dzeja PP, Miki T, Seino S and Terzic A: Knockout of Kir6.2 negates ischemic preconditioning-induced protection of myocardial energetics. Am J Physiol Heart Circ Physiol. 284:H2106–H2113. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Rainbow RD, Lodwick D, Hudman D, Davies NW, Norman RI and Standen NB: SUR2A C-terminal fragments reduce KATP currents and ischaemic tolerance of rat cardiac myocytes. J Physiol. 557:785–794. 2004. View Article : Google Scholar : PubMed/NCBI

21 

Marinovic J, Ljubkovic M, Stadnicka A, Bosnjak ZJ and Bienengraeber M: Role of sarcolemmal ATP-sensitive potassium channel in oxidative stress-induced apoptosis: mitochondrial connection. Am J Physiol Heart Circ Physiol. 294:H1317–H1325. 2008. View Article : Google Scholar : PubMed/NCBI

22 

Ohmasa F, Saito M, Oiwa H, Tsounapi P, Shomori K, Kitatani K, Dimitriadis F, Kinoshita Y and Satoh K: Pharmacological preconditioning of ATP-sensitive potassium channel openers on acute urinary retention-induced bladder dysfunction in the rat. BJU Int. 110:E245–E252. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Tsounapi P, Saito M, Dimitriadis F, Kitatani K, Kinoshita Y, Shomori K, Takenaka A and Satoh K: The role of K ATP channels on ischemia-reperfusion injury in the rat testis. Life Sci. 90:649–656. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Dorn GW II: Apoptotic and non-apoptotic programmed cardiomyocyte death in ventricular remodelling. Cardiovasc Res. 81:465–473. 2009. View Article : Google Scholar :

25 

Nishida K, Yamaguchi O and Otsu K: Crosstalk between autophagy and apoptosis in heart disease. Circ Res. 103:343–351. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Hassoun SM, Marechal X, Montaigne D, Bouazza Y, Decoster B, Lancel S and Neviere R: Prevention of endotoxin-induced sarcoplasmic reticulum calcium leak improves mitochondrial and myocardial dysfunction. Crit Care Med. 36:2590–2596. 2008. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang X, Zhang X, Xiong Y, Xu C, Liu X, Lin J, Mu G, Xu S and Liu W: Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis. Int J Mol Med 38: 758-766, 2016.
APA
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J. ... Liu, W. (2016). Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis. International Journal of Molecular Medicine, 38, 758-766. https://doi.org/10.3892/ijmm.2016.2664
MLA
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J., Mu, G., Xu, S., Liu, W."Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis". International Journal of Molecular Medicine 38.3 (2016): 758-766.
Chicago
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J., Mu, G., Xu, S., Liu, W."Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis". International Journal of Molecular Medicine 38, no. 3 (2016): 758-766. https://doi.org/10.3892/ijmm.2016.2664
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang X, Zhang X, Xiong Y, Xu C, Liu X, Lin J, Mu G, Xu S and Liu W: Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis. Int J Mol Med 38: 758-766, 2016.
APA
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J. ... Liu, W. (2016). Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis. International Journal of Molecular Medicine, 38, 758-766. https://doi.org/10.3892/ijmm.2016.2664
MLA
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J., Mu, G., Xu, S., Liu, W."Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis". International Journal of Molecular Medicine 38.3 (2016): 758-766.
Chicago
Zhang, X., Zhang, X., Xiong, Y., Xu, C., Liu, X., Lin, J., Mu, G., Xu, S., Liu, W."Sarcolemmal ATP-sensitive potassium channel protects cardiac myocytes against lipopolysaccharide-induced apoptosis". International Journal of Molecular Medicine 38, no. 3 (2016): 758-766. https://doi.org/10.3892/ijmm.2016.2664
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