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A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages

  • Authors:
    • Zhang Bao
    • Ran Chen
    • Pei Zhang
    • Shan Lu
    • Xing Chen
    • Yake Yao
    • Xiaozheng Jin
    • Yilan Sun
    • Jianying Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Zhejiang JFK Biological Technology Co., Ltd., Hangzhou, Zhejiang 310052, P.R. China
    Copyright: © Bao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 823-833
    |
    Published online on: July 11, 2016
       https://doi.org/10.3892/ijmm.2016.2675
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Abstract

Mycobacterium tuberculosis (MTB), one of the major bacterial pathogens for lethal infectious diseases, is capable of surviving within the phagosomes of host alveolar macrophages; therefore, host genetic variations may alter the susceptibility to MTB. In this study, to identify host genes exploited by MTB during infection, genes were non-selectively inactivated using lentivirus-based antisense RNA methods in Raw264.7 macrophages, and the cells that survived virulent MTB infection were then screened. Following DNA sequencing of the surviving cell clones, 26 host genes affecting susceptibility to MTB were identified and their pathways were analyzed by bioinformatics analysis. In total, 9 of these genes were confirmed as positive regulators of collagen α-5(IV) chain (Col4a5) expression, a gene encoding a type IV collagen subunit present on the cell surface. The knockdown of Col4a5 consistently suppressed intracellular mycobacterial viability, promoting the survival of Raw264.7 macrophages following mycobacterial infection. Furthermore, Col4a5 deficiency lowered the pH levels of intracellular vesicles, including endosomes, lysosomes and phagosomes in the Raw264.7 cells. Finally, the knockdown of Col4a5 post-translationally increased microsomal vacuolar-type H+-ATPase activity in macrophages, leading to the acidification of intracellular vesicles. Our findings reveal a novel role for Col4a5 in the regulation of macrophage responses to mycobacterial infection and identify Col4a5 as a potential target for the host-directed anti-mycobacterial therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Bao Z, Chen R, Zhang P, Lu S, Chen X, Yao Y, Jin X, Sun Y and Zhou J: A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages. Int J Mol Med 38: 823-833, 2016.
APA
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y. ... Zhou, J. (2016). A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages. International Journal of Molecular Medicine, 38, 823-833. https://doi.org/10.3892/ijmm.2016.2675
MLA
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y., Jin, X., Sun, Y., Zhou, J."A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages". International Journal of Molecular Medicine 38.3 (2016): 823-833.
Chicago
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y., Jin, X., Sun, Y., Zhou, J."A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages". International Journal of Molecular Medicine 38, no. 3 (2016): 823-833. https://doi.org/10.3892/ijmm.2016.2675
Copy and paste a formatted citation
x
Spandidos Publications style
Bao Z, Chen R, Zhang P, Lu S, Chen X, Yao Y, Jin X, Sun Y and Zhou J: A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages. Int J Mol Med 38: 823-833, 2016.
APA
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y. ... Zhou, J. (2016). A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages. International Journal of Molecular Medicine, 38, 823-833. https://doi.org/10.3892/ijmm.2016.2675
MLA
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y., Jin, X., Sun, Y., Zhou, J."A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages". International Journal of Molecular Medicine 38.3 (2016): 823-833.
Chicago
Bao, Z., Chen, R., Zhang, P., Lu, S., Chen, X., Yao, Y., Jin, X., Sun, Y., Zhou, J."A potential target gene for the host-directed therapy of mycobacterial infection in murine macrophages". International Journal of Molecular Medicine 38, no. 3 (2016): 823-833. https://doi.org/10.3892/ijmm.2016.2675
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