Transcriptomic changes in human renal proximal tubular cells revealed under hypoxic conditions by RNA sequencing

  • Authors:
    • Wenmin Yu
    • Yiping Li
    • Zhi Wang
    • Lei Liu
    • Jing Liu
    • Fengan Ding
    • Xiaoyi Zhang
    • Zhengyuan Cheng
    • Pingsheng Chen
    • Jun Dou
  • View Affiliations

  • Published online on: July 13, 2016     https://doi.org/10.3892/ijmm.2016.2677
  • Pages: 894-902
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Abstract

Chronic hypoxia often occurs among patients with chronic kidney disease (CKD). Renal proximal tubular cells may be the primary target of a hypoxic insult. However, the underlying transcriptional mechanisms remain undefined. In this study, we revealed the global changes in gene expression in HK‑2 human renal proximal tubular cells under hypoxic and normoxic conditions. We analyzed the transcriptome of HK‑2 cells exposed to hypoxia for 24 h using RNA sequencing. A total of 279 differentially expressed genes was examined, as these genes could potentially explain the differences in HK‑2 cells between hypoxic and normoxic conditions. Moreover, 17 genes were validated by qPCR, and the results were highly concordant with the RNA seqencing results. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to better understand the functions of these differentially expressed genes. The upregulated genes appeared to be significantly enriched in the pathyway of extracellular matrix (ECM)-receptor interaction, and in paticular, the pathway of renal cell carcinoma was upregulated under hypoxic conditions. The downregulated genes were enriched in the signaling pathway related to antigen processing and presentation; however, the pathway of glutathione metabolism was downregulated. Our analysis revealed numerous novel transcripts and alternative splicing events. Simultaneously, we also identified a large number of single nucleotide polymorphisms, which will be a rich resource for future marker development. On the whole, our data indicate that transcriptome analysis provides valuable information for a more in depth understanding of the molecular mechanisms in CKD and renal cell carcinoma.
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September-2016
Volume 38 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yu W, Li Y, Wang Z, Liu L, Liu J, Ding F, Zhang X, Cheng Z, Chen P, Dou J, Dou J, et al: Transcriptomic changes in human renal proximal tubular cells revealed under hypoxic conditions by RNA sequencing. Int J Mol Med 38: 894-902, 2016
APA
Yu, W., Li, Y., Wang, Z., Liu, L., Liu, J., Ding, F. ... Dou, J. (2016). Transcriptomic changes in human renal proximal tubular cells revealed under hypoxic conditions by RNA sequencing. International Journal of Molecular Medicine, 38, 894-902. https://doi.org/10.3892/ijmm.2016.2677
MLA
Yu, W., Li, Y., Wang, Z., Liu, L., Liu, J., Ding, F., Zhang, X., Cheng, Z., Chen, P., Dou, J."Transcriptomic changes in human renal proximal tubular cells revealed under hypoxic conditions by RNA sequencing". International Journal of Molecular Medicine 38.3 (2016): 894-902.
Chicago
Yu, W., Li, Y., Wang, Z., Liu, L., Liu, J., Ding, F., Zhang, X., Cheng, Z., Chen, P., Dou, J."Transcriptomic changes in human renal proximal tubular cells revealed under hypoxic conditions by RNA sequencing". International Journal of Molecular Medicine 38, no. 3 (2016): 894-902. https://doi.org/10.3892/ijmm.2016.2677