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Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose

  • Authors:
    • Zi-Ying Shen
    • Qian Sun
    • Zhong-Yuan Xia
    • Qing-Tao Meng
    • Shao-Qing Lei
    • Bo Zhao
    • Ling-Hua Tang
    • Rui Xue
    • Rong Chen
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, Affiliated Hospital of Qingdao University Medical College, Qingdao, Shandong 266003, P.R. China, Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Shen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 729-736
    |
    Published online on: July 14, 2016
       https://doi.org/10.3892/ijmm.2016.2680
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Abstract

Patients with diabetes are more vulnerable to renal ischemia/reperfusion (I/R) injury, which is implicated in hyperglycemia-induced oxidative stress. We previously reported that the hyperglycemia-induced inhibition of DJ-1, a novel oncogene that exhibits potent antioxidant activity, is implicated in the severity of myocardial I/R injury. In the present study, we aimed to explore the role of DJ-1 in hypoxia/reoxygenation (H/R) injury in renal cells exposed to high glucose (HG). For this purpose, NRK-52E cells were exposed to HG (30 mM) for 48 h and then exposed to hypoxia for 4 h and reoxygenation for 2 h, which significantly decreased cell viability and superoxide dismutase (SOD) activity, and increased the malondialdehyde (MDA) content, accompanied by a decrease in DJ‑1 protein expression. The overexpression of DJ‑1 by transfection with a DJ‑1 overexpression plasmid exerted protective effects against HG-induced H/R injury, as evidenced by increased CCK-8 levels and SOD activity, the decreased release of lactate dehydrogenase (LDH) and the decreased MDA content, and increased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and heme oxygenase-1 (HO‑1) expression. Similar effects were observed following treatment with the antioxidant, N-acetylcysteine. These results suggest that the overexpression of DJ‑1 reduces oxidative stress and attenuates H/R injury in NRK-52E cells exposed to HG.
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Copy and paste a formatted citation
Spandidos Publications style
Shen Z, Sun Q, Xia Z, Meng Q, Lei S, Zhao B, Tang L, Xue R and Chen R: Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose. Int J Mol Med 38: 729-736, 2016.
APA
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B. ... Chen, R. (2016). Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose. International Journal of Molecular Medicine, 38, 729-736. https://doi.org/10.3892/ijmm.2016.2680
MLA
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B., Tang, L., Xue, R., Chen, R."Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose". International Journal of Molecular Medicine 38.3 (2016): 729-736.
Chicago
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B., Tang, L., Xue, R., Chen, R."Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose". International Journal of Molecular Medicine 38, no. 3 (2016): 729-736. https://doi.org/10.3892/ijmm.2016.2680
Copy and paste a formatted citation
x
Spandidos Publications style
Shen Z, Sun Q, Xia Z, Meng Q, Lei S, Zhao B, Tang L, Xue R and Chen R: Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose. Int J Mol Med 38: 729-736, 2016.
APA
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B. ... Chen, R. (2016). Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose. International Journal of Molecular Medicine, 38, 729-736. https://doi.org/10.3892/ijmm.2016.2680
MLA
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B., Tang, L., Xue, R., Chen, R."Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose". International Journal of Molecular Medicine 38.3 (2016): 729-736.
Chicago
Shen, Z., Sun, Q., Xia, Z., Meng, Q., Lei, S., Zhao, B., Tang, L., Xue, R., Chen, R."Overexpression of DJ-1 reduces oxidative stress and attenuates hypoxia/reoxygenation injury in NRK-52E cells exposed to high glucose". International Journal of Molecular Medicine 38, no. 3 (2016): 729-736. https://doi.org/10.3892/ijmm.2016.2680
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