Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
September-2016 Volume 38 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2016 Volume 38 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines

  • Authors:
    • Chao Hou
    • Qianni Wu
    • Chen Ouyang
    • Ting Huang
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong 510030, P.R. China
    Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 713-720
    |
    Published online on: July 22, 2016
       https://doi.org/10.3892/ijmm.2016.2688
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

In order to explore the potential effects of interleukin (IL)-35 on IL-10, transforming growth factor-β (TGF-β), interferon-γ (INF)-γ, IL-12 and IL-17, a pcDNA3.1‑IL-35 plasmid was injected into the vitreous cavity of BALB/c mice. Enzyme-linked immunosorbent assay, western blot analysis and quantitative PCR analysis were performed to confirm the successful expression of IL-35. Slit-lamp biomicroscopy, hematoxylin and eosin staining and immunofluorescence were employed to detect the status of eyes, and western blot analysis was performed to examine the expression of corneal graft rejection-related cytokines. There were no abnormalities in the eyes pre-mydriasis or post-mydriasis and no injuries to the cornea or retina following the injection of IL-35-expressing plasmid. An immunofluorescence assay detected the positive expression of IL-35 in corneal epithelial cells from IL-35‑injected mice and negative staining in the control group. Further study revealed that IL-35 enhanced the expression of IL-10 and TGF-β which reached their highest levels at 1 and 2 weeks after injection, respectively (p<0.01). Moreover, the expression of INF-γ and IL-12 was decreased significantly at 2 weeks after the injection of IL-35-expressing plasmid (p<0.05), and the expression of IL-17 was suppressed notably at 4 weeks after the injection (p<0.05). The intravitreal injection of IL-35-expressing plasmid in mice downregulates the expression of pro-inflammatory cytokines and upregulates the expression of anti-inflammatory cytokines. Thus, IL-35 may further be assessed as a potential target for the treatment of corneal graft rejection.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Qin Q, Shi Y, Zhao Q, Luo D, Chen Y, Wu J and Zhao M: Effects of CD25siRNA gene transfer on high-risk rat corneal graft rejection. Graefes Arch Clin Exp Ophthalmol. 253:1765–1776. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Pan Q, Xu Q, Boylan NJ, Lamb NW, Emmert DG, Yang JC, Tang L, Heflin T, Alwadani S, Eberhart CG, et al: Corticosteroid-loaded biodegradable nanoparticles for prevention of corneal allograft rejection in rats. J Control Release. 201:32–40. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Chen Y, Liao C, Gao M, Belin MW, Wang M, Yu H and Yu J: Efficacy and safety of corneal transplantation using corneas from foreign donors versus domestic donors: a prospective, randomized, controlled trial. J Ophthalmol. 2015:1782892015. View Article : Google Scholar : PubMed/NCBI

4 

Paunicka KJ, Mellon J, Robertson D, Petroll M, Brown JR and Niederkorn JY: Severing corneal nerves in one eye induces sympathetic loss of immune privilege and promotes rejection of future corneal allografts placed in either eye. Am J Transplant. 15:1490–1501. 2015. View Article : Google Scholar : PubMed/NCBI

5 

Zhang H, Wang L and Zhang L: Cyclosporine nanomicelle eye drop: a novel medication for corneal graft transplantation treatment. Biol Pharm Bull. 38:893–900. 2015. View Article : Google Scholar : PubMed/NCBI

6 

Pleyer U, Milani JK, Dukes A, Chou J, Lutz S, Rückert D, Thiel HJ and Mondino BJ: Effect of topically applied anti-CD4 monoclonal antibodies on orthotopic corneal allografts in a rat model. Invest Ophthalmol Vis Sci. 36:52–61. 1995.PubMed/NCBI

7 

Niederkorn JY: Immunology and immunomodulation of corneal transplantation. Int Rev Immunol. 21:173–196. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Olson BM, Sullivan JA and Burlingham WJ: Interleukin 35: a key mediator of suppression and the propagation of infectious tolerance. Front Immunol. 4:3152013. View Article : Google Scholar : PubMed/NCBI

9 

Käser T, Müllebner A, Hartl RT, Essler SE, Saalmüller A and Catharina Duvigneau J: Porcine T-helper and regulatory T cells exhibit versatile mRNA expression capabilities for cytokines and co-stimulatory molecules. Cytokine. 60:400–409. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Zhang YL, Zhou XY, Guo XY and Tu JW: Association between serum interleukin-35 levels and severity of acute pancreatitis. Int J Clin Exp Med. 8:7430–7434. 2015.PubMed/NCBI

11 

Filková M, Vernerová Z, Hulejová H, Prajzlerová K, Veigl D, Pavelka K, Vencovský J and Šenolt L: Pro-inflammatory effects of interleukin-35 in rheumatoid arthritis. Cytokine. 73:36–43. 2015. View Article : Google Scholar : PubMed/NCBI

12 

Köseoğlu S, Sağlam M, Pekbağrıyanık T, Savran L and Sütçü R: Level of interleukin-35 in gingival crevicular fluid, saliva, and plasma in periodontal disease and health. J Periodontol. 86:964–971. 2015. View Article : Google Scholar

13 

Ding LF, Chen Q, Li L, Liu JM, Zhang GP, Zhu XH, Wu AM, Ke JW, Dai YL and Wu CX: Effects of sublingual immunotherapy on serum IL-17 and IL-35 levels in children with allergic rhinitis or asthma. Zhongguo Dang Dai Er Ke Za Zhi. 16:1206–1210. 2014.In Chinese. PubMed/NCBI

14 

Collison LW, Chaturvedi V, Henderson AL, Giacomin PR, Guy C, Bankoti J, Finkelstein D, Forbes K, Workman CJ, Brown SA, et al: IL-35-mediated induction of a potent regulatory T cell population. Nat Immunol. 11:1093–1101. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Collison LW, Workman CJ, Kuo TT, Boyd K, Wang Y, Vignali KM, Cross R, Sehy D, Blumberg RS and Vignali DA: the inhibitory cytokine IL-35 contributes to regulatory T-cell function. Nature. 450:566–569. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Tsuda M, Zhang W, Yang GX, Tsuneyama K, Ando Y, Kawata K, Park O, Leung PS, Coppel RL, Ansari AA, et al: Deletion of interleukin (IL)-12p35 induces liver fibrosis in dominant-negative TGFβ receptor type II mice. Hepatology. 57:806–816. 2013. View Article : Google Scholar

17 

Wirtz S, Billmeier U, Mchedlidze T, Blumberg RS and Neurath MF: Interleukin-35 mediates mucosal immune responses that protect against T-cell-dependent colitis. Gastroenterology. 141:1875–1886. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Guo H, Wang W, Zhao N, He X, Zhu L and Jiang X: Inhibiting cardiac allograft rejection with interleukin-35 therapy combined with decitabine treatment in mice. Transpl Immunol. 29:99–104. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Jin E, Wang C, Hu Q, Jin G and Li S: The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy. Rom J Morphol Embryol. 55:1353–1361. 2014.

20 

Ali TK, Matragoon S, Pillai BA, Liou GI and El-Remessy AB: Peroxynitrite mediates retinal neurodegeneration by inhibiting nerve growth factor survival signaling in experimental and human diabetes. Diabetes. 57:889–898. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Niedbala W, Wei XQ, Cai B, Hueber AJ, Leung BP, McInnes IB and Liew FY: IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells. Eur J Immunol. 37:3021–3029. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Hori S, Nomura T and Sakaguchi S: Control of regulatory T cell development by the transcription factor Foxp3. Science. 299:1057–1061. 2003. View Article : Google Scholar : PubMed/NCBI

23 

He Y, Jie Y, Wang B, Zeng H, Zhang Y and Pan Z: Adoptive transfer of donor corneal antigen-specific regulatory T cells can prolong mice corneal grafts survival. Cornea. 29(Suppl 1): S25–S31. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Rossmanith W, Chabicovsky M, Herkner K and Schulte-Hermann R: Cellular gene dose and kinetics of gene expression in mouse livers transfected by high-volume tail-vein injection of naked DNA. DNA Cell Biol. 21:847–853. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Maruyama H, Higuchi N, Nishikawa Y, Kameda S, Iino N, Kazama JJ, Takahashi N, Sugawa M, Hanawa H, Tada N, et al: High-level expression of naked DNA delivered to rat liver via tail vein injection. J Gene Med. 4:333–341. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Liu F, Song Y and Liu D: Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 6:1258–1266. 1999. View Article : Google Scholar : PubMed/NCBI

27 

Andrianaivo F, Lecocq M, Wattiaux-De Coninck S, Wattiaux R and Jadot M: Hydrodynamics-based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection. J Gene Med. 6:877–883. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Jiang Y, Zhang Q and Steinle JJ: Intravitreal injection of IGFBP-3 restores normal insulin signaling in diabetic rat retina. PLoS One. 9:e937882014. View Article : Google Scholar : PubMed/NCBI

29 

Yoon KC, Ahn KY, Lee JH, Chun BJ, Park SW, Seo MS, Park YG and Kim KK: Lipid-mediated delivery of brain-specific angiogenesis inhibitor 1 gene reduces corneal neovascularization in an in vivo rabbit model. Gene Ther. 12:617–624. 2005. View Article : Google Scholar : PubMed/NCBI

30 

Sonoda S, Tachibana K, Uchino E, Okubo A, Yamamoto M, Sakoda K, Hisatomi T, Sonoda KH, Negishi Y, Izumi Y, et al: Gene transfer to corneal epithelium and keratocytes mediated by ultrasound with microbubbles. Invest Ophthalmol Vis Sci. 47:558–564. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Masli S, Turpie B, Hecker KH and Streilein JW: Expression of thrombospondin in TGFbeta-treated APCs and its relevance to their immune deviation-promoting properties. J Immunol. 168:2264–2273. 2002. View Article : Google Scholar : PubMed/NCBI

32 

Torres PF, De Vos AF, van der Gaag R, Martins B and Kijlstra A: Cytokine mRNA expression during experimental corneal allograft rejection. Exp Eye Res. 63:453–461. 1996. View Article : Google Scholar : PubMed/NCBI

33 

Song SS, Yuan PF, Chen JY, Fu JJ, Wu HX, Lu JT and Wei W: TGF-β favors bone marrow-derived dendritic cells to acquire tolerogenic properties. Immunol Invest. 43:360–369. 2014. View Article : Google Scholar

34 

Zhou L, Zhu X, Tan J, Wang J and Xing Y: Effect of recombinant adeno-associated virus mediated transforming growth factor-beta1 on corneal allograft survival after high-risk penetrating keratoplasty. Transpl Immunol. 28:164–169. 2013. View Article : Google Scholar : PubMed/NCBI

35 

Enzmann V, Hollborn M, Wiedemann P and Kohen L: Minor influence of the immunosuppressive cytokines IL-10 and TGF-beta on the proliferation and apoptosis of human retinal pigment epithelial (RPE) cells in vitro. Ocul Immunol Inflamm. 9:259–266. 2001. View Article : Google Scholar

36 

Li B, Tian L, Diao Y, Li X, Zhao L and Wang X: Exogenous IL-10 induces corneal transplantation immune tolerance by a mechanism associated with the altered Th1/Th2 cytokine ratio and the increased expression of TGF-β. Mol Med Rep. 9:2245–2250. 2014.PubMed/NCBI

37 

Klebe S, Sykes PJ, Coster DJ, Krishnan R and Williams KA: Prolongation of sheep corneal allograft survival by ex vivo transfer of the gene encoding interleukin-10. Transplantation. 71:1214–1220. 2001. View Article : Google Scholar : PubMed/NCBI

38 

Wang X, Wang W, Xu J, Wu S and Le Q: All-trans retinoid acid promotes allogeneic corneal graft survival in mice by regulating Treg-Th17 balance in the presence of TGF-β. BMC Immunol. 16:172015. View Article : Google Scholar

39 

Klebe S, Coster DJ, Sykes PJ, Swinburne S, Hallsworth P, Scheerlinck JP, Krishnan R and Williams KA: Prolongation of sheep corneal allograft survival by transfer of the gene encoding ovine IL-12-p40 but not IL-4 to donor corneal endothelium. J Immunol. 175:2219–2226. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Gee K, Guzzo C, Che Mat NF, Ma W and Kumar A: The IL-12 family of cytokines in infection, inflammation and autoimmune disorders. Inflamm Allergy Drug Targets. 8:40–52. 2009. View Article : Google Scholar : PubMed/NCBI

41 

King WJ, Comer RM, Hudde T, Larkin DF and George AJ: Cytokine and chemokine expression kinetics after corneal transplantation. Transplantation. 70:1225–1233. 2000. View Article : Google Scholar : PubMed/NCBI

42 

Maier P, Heizmann U, Böhringer D, Kern Y and Reinhard T: Predicting the risk for corneal graft rejection by aqueous humor analysis. Mol Vis. 17:1016–1023. 2011.PubMed/NCBI

43 

Park H, Li Z, Yang XO, Chang SH, Nurieva R, Wang YH, Wang Y, Hood L, Zhu Z, Tian Q and Dong C: A distinct lineage of CD4 T cells regulates tissue inflammation by producing interleukin 17. Nat Immunol. 6:1133–1141. 2005. View Article : Google Scholar : PubMed/NCBI

44 

Tang JL, Subbotin VM, Antonysamy MA, Troutt AB, Rao AS and Thomson AW: Interleukin-17 antagonism inhibits acute but not chronic vascular rejection. Transplantation. 72:348–350. 2001. View Article : Google Scholar : PubMed/NCBI

45 

Antonysamy MA, Fanslow WC, Fu F, Li W, Qian S, Troutt AB and Thomson AW: Evidence for a role of IL-17 in alloimmunity: a novel IL-17 antagonist promotes heart graft survival. Transplant Proc. 31:931999. View Article : Google Scholar : PubMed/NCBI

46 

Chen X, Zhao S, Tang X, Ge H and Liu P: Neutralization of mouse interleukin-17 bioactivity inhibits corneal allograft rejection. Mol Vis. 17:2148–2156. 2011.PubMed/NCBI

47 

Chen H, Wang W, Xie H, Xu X, Wu J, Jiang Z, Zhang M, Zhou L and Zheng S: A pathogenic role of IL-17 at the early stage of corneal allograft rejection. Transpl Immunol. 21:155–161. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hou C, Wu Q, Ouyang C and Huang T: Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med 38: 713-720, 2016.
APA
Hou, C., Wu, Q., Ouyang, C., & Huang, T. (2016). Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. International Journal of Molecular Medicine, 38, 713-720. https://doi.org/10.3892/ijmm.2016.2688
MLA
Hou, C., Wu, Q., Ouyang, C., Huang, T."Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines". International Journal of Molecular Medicine 38.3 (2016): 713-720.
Chicago
Hou, C., Wu, Q., Ouyang, C., Huang, T."Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines". International Journal of Molecular Medicine 38, no. 3 (2016): 713-720. https://doi.org/10.3892/ijmm.2016.2688
Copy and paste a formatted citation
x
Spandidos Publications style
Hou C, Wu Q, Ouyang C and Huang T: Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. Int J Mol Med 38: 713-720, 2016.
APA
Hou, C., Wu, Q., Ouyang, C., & Huang, T. (2016). Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines. International Journal of Molecular Medicine, 38, 713-720. https://doi.org/10.3892/ijmm.2016.2688
MLA
Hou, C., Wu, Q., Ouyang, C., Huang, T."Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines". International Journal of Molecular Medicine 38.3 (2016): 713-720.
Chicago
Hou, C., Wu, Q., Ouyang, C., Huang, T."Effects of an intravitreal injection of interleukin-35-expressing plasmid on pro-inflammatory and anti-inflammatory cytokines". International Journal of Molecular Medicine 38, no. 3 (2016): 713-720. https://doi.org/10.3892/ijmm.2016.2688
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team