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Article

Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation

  • Authors:
    • Bailing Chen
    • Tao Lin
    • Xiaoxi Yang
    • Yiqiang Li
    • Denghui Xie
    • Wenhui Zheng
    • Haowen Cui
    • Weimin Deng
    • Xin Tan
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Spine Surgery, Chinese PLA General Hospital (301 Hospital), Beijing 100853, P.R. China, Department of Orthopedics, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong 510623, P.R. China, Department of Spine Surgery, The Third Affiliated Hospital of Southern Medical University (Academy of Orthopedics), Guangzhou, Guangdong 510630, P.R. China, Department of Physiology, Zhongshan School of Medicine of Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China, Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, Guangdong 510000, P.R. China
  • Pages: 1531-1540
    |
    Published online on: September 28, 2016
       https://doi.org/10.3892/ijmm.2016.2757
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Abstract

The positive effect of low-magnitude, high‑frequency (LMHF) vibration on implant osseointegration has been demonstrated; however, the underlying cellular and molecular mechanisms remain unknown. The aim of this study was to explore the effect of LMHF vibration on the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) cultured on hydroxyapatite (HA)-coated surfaces in an in vitro model as well as to elucidate the molecular mechanism responsible for the effects of LMHF vibration on osteogenesis. LMHF vibration resulted in the increased expression of fibronectin, which was measured by immunostaining and RT-qPCR. Stimulation of BMSCs by LMHF vibration resulted in the rearrangement of the actin cytoskeleton with more prominent F-actin. Moreover, the expression of β1 integrin, vinculin and paxillin was notably increased following LMHF stimulation. Scanning electron microscope observations revealed that there were higher cell numbers and more extracellular matrix attached to the HA-coated surface in the LMHF group. Alkaline phosphatase activity as well as the expression of osteogenic-specific genes, namely Runx2, osterix, collagen I and osteocalcin, were significantly elevated in the LMHF group. In addition, the protein expression of Wnt10B, β-catenin, Runx2 and osterix was increased following exposure to LMHF vibration. Taken together, the findings of this study indicate that LMHF vibration promotes the adhesion and the osteogenic differentiation of BMSCs on HA-coated surfaces in vitro, and LMHF vibration may directly induce osteogenesis by activating the Wnt/β‑catenin signaling pathway. These data suggest that LMHF vibration enhances the osseointegration of bone to a HA-coated implant, and provide a scientific foundation for improving bone-implant osseointegration through the application of LMHF vibration.
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Copy and paste a formatted citation
Spandidos Publications style
Chen B, Lin T, Yang X, Li Y, Xie D, Zheng W, Cui H, Deng W and Tan X: Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation. Int J Mol Med 38: 1531-1540, 2016.
APA
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W. ... Tan, X. (2016). Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation. International Journal of Molecular Medicine, 38, 1531-1540. https://doi.org/10.3892/ijmm.2016.2757
MLA
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W., Cui, H., Deng, W., Tan, X."Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation". International Journal of Molecular Medicine 38.5 (2016): 1531-1540.
Chicago
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W., Cui, H., Deng, W., Tan, X."Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation". International Journal of Molecular Medicine 38, no. 5 (2016): 1531-1540. https://doi.org/10.3892/ijmm.2016.2757
Copy and paste a formatted citation
x
Spandidos Publications style
Chen B, Lin T, Yang X, Li Y, Xie D, Zheng W, Cui H, Deng W and Tan X: Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation. Int J Mol Med 38: 1531-1540, 2016.
APA
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W. ... Tan, X. (2016). Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation. International Journal of Molecular Medicine, 38, 1531-1540. https://doi.org/10.3892/ijmm.2016.2757
MLA
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W., Cui, H., Deng, W., Tan, X."Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation". International Journal of Molecular Medicine 38.5 (2016): 1531-1540.
Chicago
Chen, B., Lin, T., Yang, X., Li, Y., Xie, D., Zheng, W., Cui, H., Deng, W., Tan, X."Low-magnitude, high-frequency vibration promotes the adhesion and the osteogenic differentiation of bone marrow-derived mesenchymal stem cells cultured on a hydroxyapatite-coated surface: The direct role of Wnt/β-catenin signaling pathway activation". International Journal of Molecular Medicine 38, no. 5 (2016): 1531-1540. https://doi.org/10.3892/ijmm.2016.2757
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