Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells

Yang,Da-Wun; Kang,Ok-Hwa; Lee,Young-Seob; Han,Sin-Hee; Lee,Sang-Won; Cha,Seon‑Woo; Seo,Yun-Soo; Mun,Su-Hyun; Gong,Ryong; Shin,Dong-Won; Kwon,Dong-Yeul

doi:10.3892/ijmm.2016.2781

Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells

Authors:
- Da-Wun Yang
- Ok-Hwa Kang
- Young-Seob Lee
- Sin-Hee Han
- Sang-Won Lee
- Seon‑Woo Cha
- Yun-Soo Seo
- Su-Hyun Mun
- Ryong Gong
- Dong-Won Shin
- Dong-Yeul Kwon
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Affiliations: BK21 Plus Team, Professional Graduate School of Oriental Medicine,Wonkwang University, Iksan, Jeonbuk 570‑749, Republic of Korea, Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 369-873, Republic of Korea, Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea
Published online on: October 18, 2016 https://doi.org/10.3892/ijmm.2016.2781
Pages: 1864-1870

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Abstract

Salidroside [2-(4-hydroxyphenyl)ethyl β-D-glucopyranoside (SAS)] has been identified as the most potent ingredient of the plant Rhodiola rosea L. Previous studies have demonstrated that it possesses a number of pharmacological properties, including anti-aging, anti-fatigue, antioxidant, anticancer and anti-inflammatory properties. In this study, to ascertain the molecular mechanisms responsible for the anti-inflammatory activity of SAS, we used phorbol-12-myristate-13-acetate (PMA) plus A23187 to induce inflammation in human mast cell line-1 (HMC-1). The HMC-1 cells were treated with SAS prior to being stimulated with PMA plus A23187. Pro-inflammatory cytokine production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis was used to examine the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). SAS inhibited the mRNA expression and production of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF). In cells stimulated with PMA plus A23187, SAS suppressed the phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-jun N-terminal kinase 1/2 (JNK1/2), but not that of p38 MAPK. SAS suppressed the expression of NF-κB in the nucleus. On the whole, our results suggest that SAS exerts an anti-inflammatory effect by inhibiting the production of pro-inflammatory cytokines through the blocking of the NF-κB and MAPK signaling pathways.

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