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Article

Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells

  • Authors:
    • Da-Wun Yang
    • Ok-Hwa Kang
    • Young-Seob Lee
    • Sin-Hee Han
    • Sang-Won Lee
    • Seon‑Woo Cha
    • Yun-Soo Seo
    • Su-Hyun Mun
    • Ryong Gong
    • Dong-Won Shin
    • Dong-Yeul Kwon
  • View Affiliations / Copyright

    Affiliations: BK21 Plus Team, Professional Graduate School of Oriental Medicine,Wonkwang University, Iksan, Jeonbuk 570‑749, Republic of Korea, Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Institute of Biotechnology, Wonkwang University, Iksan, Jeonbuk 570-749, Republic of Korea, Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, Chungbuk 369-873, Republic of Korea, Department of Oriental Medicine Resources, Sunchon National University, Jeonnam 540-742, Republic of Korea
  • Pages: 1864-1870
    |
    Published online on: October 18, 2016
       https://doi.org/10.3892/ijmm.2016.2781
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Abstract

Salidroside [2-(4-hydroxyphenyl)ethyl β-D-gluco­pyranoside (SAS)] has been identified as the most potent ingredient of the plant Rhodiola rosea L. Previous studies have demonstrated that it possesses a number of pharmacological properties, including anti-aging, anti-fatigue, antioxidant, anticancer and anti-inflammatory properties. In this study, to ascertain the molecular mechanisms responsible for the anti-inflammatory activity of SAS, we used phorbol-12-myristate-13-acetate (PMA) plus A23187 to induce inflammation in human mast cell line-1 (HMC-1). The HMC-1 cells were treated with SAS prior to being stimulated with PMA plus A23187. Pro-inflammatory cytokine production was measured by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis was used to examine the activation of mitogen-activated protein kinases (MAPKs) and nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB). SAS inhibited the mRNA expression and production of interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF). In cells stimulated with PMA plus A23187, SAS suppressed the phosphorylation of extracellular signal-regulated kinase (ERK)1/2 and c-jun N-terminal kinase 1/2 (JNK1/2), but not that of p38 MAPK. SAS suppressed the expression of NF-κB in the nucleus. On the whole, our results suggest that SAS exerts an anti-inflammatory effect by inhibiting the production of pro-inflammatory cytokines through the blocking of the NF-κB and MAPK signaling pathways.
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Copy and paste a formatted citation
Spandidos Publications style
Yang D, Kang O, Lee Y, Han S, Lee S, Cha SW, Seo Y, Mun S, Gong R, Shin D, Shin D, et al: Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells. Int J Mol Med 38: 1864-1870, 2016.
APA
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S. ... Kwon, D. (2016). Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells. International Journal of Molecular Medicine, 38, 1864-1870. https://doi.org/10.3892/ijmm.2016.2781
MLA
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S., Seo, Y., Mun, S., Gong, R., Shin, D., Kwon, D."Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells". International Journal of Molecular Medicine 38.6 (2016): 1864-1870.
Chicago
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S., Seo, Y., Mun, S., Gong, R., Shin, D., Kwon, D."Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells". International Journal of Molecular Medicine 38, no. 6 (2016): 1864-1870. https://doi.org/10.3892/ijmm.2016.2781
Copy and paste a formatted citation
x
Spandidos Publications style
Yang D, Kang O, Lee Y, Han S, Lee S, Cha SW, Seo Y, Mun S, Gong R, Shin D, Shin D, et al: Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells. Int J Mol Med 38: 1864-1870, 2016.
APA
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S. ... Kwon, D. (2016). Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells. International Journal of Molecular Medicine, 38, 1864-1870. https://doi.org/10.3892/ijmm.2016.2781
MLA
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S., Seo, Y., Mun, S., Gong, R., Shin, D., Kwon, D."Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells". International Journal of Molecular Medicine 38.6 (2016): 1864-1870.
Chicago
Yang, D., Kang, O., Lee, Y., Han, S., Lee, S., Cha, S., Seo, Y., Mun, S., Gong, R., Shin, D., Kwon, D."Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells". International Journal of Molecular Medicine 38, no. 6 (2016): 1864-1870. https://doi.org/10.3892/ijmm.2016.2781
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