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Article

Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure

  • Authors:
    • Xiang-Yang Fang
    • Zhe Chen
    • Guo-Bin Miao
    • Lin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Internal Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China, Heart Center, Beijing Tsinghua Changgung Hospital, Beijing 102218, P.R. China, Heart Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China
  • Pages: 1933-1939
    |
    Published online on: October 24, 2016
       https://doi.org/10.3892/ijmm.2016.2786
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Abstract

β-adrenergic receptors (β-ARs) and anti-β1-AR autoantibodies play important roles in heart failure. This study was designed to investigate the expression of β1- and β2-ARs in the lungs, and their relevance to the corresponding autoantibodies in an aged rat model of heart failure. In addition, we investigated the association between anti-β-AR autoantibody and soluble Fas (sFas) and soluble Fas ligand (sFasL). Aged male Wistar rats were divided into the sham-operated control group and the heart failure group. At 0 and 9 weeks post-surgery, the protein levels of β1- and β2-ARs in the heart and lungs were measured by western blot analysis. The plasma concentrations of autoantibodies, sFas and sFasL were determined by enzyme-linked immunosorbent assay (ELISA). The protein levels of pulmonary β1- and β2-ARs were decreased in the heart failure group when compared with the control group (P<0.01). Both the frequencies of the occurrence and the titers of autoantibodies against β2-AR increased at 9 weeks post-surgery (P<0.01). The levels of sFas and sFasL were also elevated, although there was no difference in the levels of sFas and sFasL between the groups, with positive and negative anti-β-AR autoantibody. These findings suggested that during the development of heart failure, the densities of pulmonary β1- and β2-ARs decreased. The levels of anti-β2-AR autoantibody exhibited similar changes as those of anti-β1-AR autoantibody, and there was no definite association between anti-β-AR autoantibody and the levels of sFas/sFasL.
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Copy and paste a formatted citation
Spandidos Publications style
Fang X, Chen Z, Miao G and Zhang L: Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure. Int J Mol Med 38: 1933-1939, 2016.
APA
Fang, X., Chen, Z., Miao, G., & Zhang, L. (2016). Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure. International Journal of Molecular Medicine, 38, 1933-1939. https://doi.org/10.3892/ijmm.2016.2786
MLA
Fang, X., Chen, Z., Miao, G., Zhang, L."Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure". International Journal of Molecular Medicine 38.6 (2016): 1933-1939.
Chicago
Fang, X., Chen, Z., Miao, G., Zhang, L."Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure". International Journal of Molecular Medicine 38, no. 6 (2016): 1933-1939. https://doi.org/10.3892/ijmm.2016.2786
Copy and paste a formatted citation
x
Spandidos Publications style
Fang X, Chen Z, Miao G and Zhang L: Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure. Int J Mol Med 38: 1933-1939, 2016.
APA
Fang, X., Chen, Z., Miao, G., & Zhang, L. (2016). Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure. International Journal of Molecular Medicine, 38, 1933-1939. https://doi.org/10.3892/ijmm.2016.2786
MLA
Fang, X., Chen, Z., Miao, G., Zhang, L."Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure". International Journal of Molecular Medicine 38.6 (2016): 1933-1939.
Chicago
Fang, X., Chen, Z., Miao, G., Zhang, L."Expression of β1- and β2-adrenergic receptors in the lungs and changes in the levels of corresponding autoantibodies in an aged rat model of heart failure". International Journal of Molecular Medicine 38, no. 6 (2016): 1933-1939. https://doi.org/10.3892/ijmm.2016.2786
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