Open Access

Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach

  • Authors:
    • Haiwei Yang
    • Hao Chen
    • Min Jin
    • Hua Xie
    • Shaoheng He
    • Ji-Fu Wei
  • View Affiliations

  • Published online on: October 27, 2016     https://doi.org/10.3892/ijmm.2016.2793
  • Pages: 1795-1805
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Per a 9 is a major allergen of the American cockroach (CR), which has been recognized as an important cause of imunoglobulin E-mediated type I hypersensitivity worldwide. However, it is not neasy to obtain a substantial quantity of this allergen for use in functional studies. In the present study, the Per a 9 gene was cloned and expressed in Escherichia coli (E. coli) systems. It was found that 13/16 (81.3%) of the sera from patients with allergies caused by the American CR reacted to Per a 9, as assessed by enzyme-linked immunosorbent assay, confirming that Per a 9 is a major allergen of CR. The induction of the expression of CD63 and CCR3 in passively sensitized basophils (from sera of patients with allergies caused by the American CR) by approximately 4.2-fold indicated that recombinant Per a 9 was functionally active. Three immunoinformatics tools, including the DNAStar Protean system, Bioinformatics Predicted Antigenic Peptides (BPAP) system and the BepiPred 1.0 server were used to predict the potential B cell epitopes, while Net-MHCIIpan-2.0 and NetMHCII-2.2 were used to predict the T cell epitopes of Per a 9. As a result, we predicted 11 peptides (23-28, 39-46, 58-64, 91-118, 131-136, 145-154, 159-165, 176-183, 290-299, 309-320 and 338-344) as potential B cell linear epitopes. In T cell prediction, the Per a 9 allergen was predicted to have 5 potential T cell epitope sequences, 119-127, 194-202, 210-218, 239-250 and 279-290. The findings of our study may prove to be useful in the development of peptide-based vaccines to combat CR-induced allergies.
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December-2016
Volume 38 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Yang H, Chen H, Jin M, Xie H, He S and Wei J: Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach. Int J Mol Med 38: 1795-1805, 2016
APA
Yang, H., Chen, H., Jin, M., Xie, H., He, S., & Wei, J. (2016). Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach. International Journal of Molecular Medicine, 38, 1795-1805. https://doi.org/10.3892/ijmm.2016.2793
MLA
Yang, H., Chen, H., Jin, M., Xie, H., He, S., Wei, J."Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach". International Journal of Molecular Medicine 38.6 (2016): 1795-1805.
Chicago
Yang, H., Chen, H., Jin, M., Xie, H., He, S., Wei, J."Molecular cloning, expression, IgE binding activities and in silico epitope prediction of Per a 9 allergens of the American cockroach". International Journal of Molecular Medicine 38, no. 6 (2016): 1795-1805. https://doi.org/10.3892/ijmm.2016.2793