Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma

  • Authors:
    • Kaituo Gao
    • Jijuan Yin
    • Jian Dong
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  • Published online on: November 10, 2016     https://doi.org/10.3892/ijmm.2016.2800
  • Pages: 1850-1856
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Abstract

WW domain-containing oxidoreductase (WWOX) is frequently inactivated in human osteosarcoma, and the restoration of its expression can suppress tumorigenicity in WWOX-negative OS cells. However, its regulatory mechanisms remain to be fully elucidated. In the present study, we demonstrate that WWOX is downregulated and that it regulates proliferation and epithelial-to-mesenchymal transition (EMT)-associated protein expression in osteosarcoma. As shown by our results, WWOX overexpression by transfection with WWOX overexpression plasmids suppressed the proliferation, migration and invasion of osteosarcoma MG63 cells (as shown by MTT and migration and invasion assays). The silencing of microRNA (miR)‑214‑3p by transfection with anti-miR‑14‑3p upregulated WWOX protein expression and also inhibited the proliferation, migration and invasion of osteosarcoma cells. Additionally, we found that WWOX negatively regulated miR‑214‑3p and miR‑10b expression. Our findings define a negative feedback pathway in control of WWOX and miR‑214‑3p expression, thus providing novel molecular targets for the treatment of osteosarcoma.
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December-2016
Volume 38 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Gao K, Yin J and Dong J: Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma. Int J Mol Med 38: 1850-1856, 2016
APA
Gao, K., Yin, J., & Dong, J. (2016). Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma. International Journal of Molecular Medicine, 38, 1850-1856. https://doi.org/10.3892/ijmm.2016.2800
MLA
Gao, K., Yin, J., Dong, J."Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma". International Journal of Molecular Medicine 38.6 (2016): 1850-1856.
Chicago
Gao, K., Yin, J., Dong, J."Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma". International Journal of Molecular Medicine 38, no. 6 (2016): 1850-1856. https://doi.org/10.3892/ijmm.2016.2800