Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
International Journal of Molecular Medicine
Join Editorial Board Propose a Special Issue
Print ISSN: 1107-3756 Online ISSN: 1791-244X
Journal Cover
January-2017 Volume 39 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2017 Volume 39 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27

  • Authors:
    • Cho-Rong Kim
    • Young-Min Kim
    • Min-Kyeong Lee
    • In-Hye Kim
    • Youn-Hee Choi
    • Taek-Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Nutrition, Pukyong National University, Busan 48513, Republic of Korea, Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Republic of Korea
    Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 31-38
    |
    Published online on: November 18, 2016
       https://doi.org/10.3892/ijmm.2016.2807
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Pyropia yezoensis (P. yezoensis) is a marine algae that exhibits antioxidant, anti-inflammatory, antitumor and anti-aging activities. In this study, we investigated the effects of the P. yezoensis peptide, PYP1‑5, on collagen synthesis in the human dermal fibroblast cell line Hs27. Skin aging is related to reduced collagen production and the activities of multiple enzymes, including matrix metalloproteinases (MMPs), which degrade collagen structure in the dermis, and tissue inhibitor of tissue inhibitor of metalloproteinases (TIMPs), which inhibit the action of MMPs. While collagen synthesis is associated with a number of signaling pathways, we examined the increased collagen synthesis via the upregulation of the transforming growth factor-β (TGF-β)/Smad signaling pathway. Using MTS assay, we found that PYP1‑5 did not affect cell viability. Moreover, we confirmed that PYP1‑5 increased type 1 collagen expression using enzyme-linked immunosorbent assay (ELISA), western blot analysis and quantitative PCR. In addition, we identified changes in various enzymes, as well as the mechanisms behind the PYP1‑5-induced collagen synthesis. PYP1‑5 decreased the MMP-1 protein and mRNA levels, and increased the TIMP-1 and TIMP-2 protein and mRNA levels. In addition, PYP1‑5 activated the TGF-β/Smad signaling pathway, which increased TGF-β1, p-Smad2 and p-Smad3 expression, while inhibiting Smad7, an inhibitor of the TGF-β/Smad pathway. Furthermore, PYP1‑5 upregulated transcription factor specificity protein 1 (Sp1) expression, which is reportedly involved in type 1 collagen expression. These findings indicate that PYP1‑5 activates the TGF-β/Smad signaling pathway, which subsequently induces collagen synthesis in Hs27 cells.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Kim SH, Yong HJ, Shin C and Ko SG: Research of traditional herbal medicines for anti-aging, inhibition effect of wrinkle and whitening effect in the skin. Kor J Ori Physiol Pathol. 22:691–698. 2008.

2 

Mukherjee PK, Maity N, Nema NK and Sarkar BK: Bioactive compounds from natural resources against skin aging. Phytomedicine. 19:64–73. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Farage MA, Miller KW, Elsner P and Maibach HI: Intrinsic and extrinsic factors in skin ageing: A review. Int J Cosmet Sci. 30:87–95. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Naylor EC, Watson RE and Sherratt MJ: Molecular aspects of skin ageing. Maturitas. 69:249–256. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Costin GE and Hearing VJ: Human skin pigmentation: Melanocytes modulate skin color in response to stress. FASEB J. 21:976–994. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Yoon JH, Kim J, Lee H, Kim SY, Jang HH, Ryu SH, Kim BJ and Lee TG: Laminin peptide YIGSR induces collagen synthesis in Hs27 human dermal fibroblasts. Biochem Biophys Res Commun. 428:416–421. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Kim EJ, Kim MK, Jin XJ, Oh JH, Kim JE and Chung JH: Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin. J Korean Med Sci. 25:980–983. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Kähäri VM and Saarialho-Kere U: Matrix metalloproteinases in skin. Exp Dermatol. 6:199–213. 1997. View Article : Google Scholar

9 

Dennler S, Goumans MJ and ten Dijke P: Transforming growth factor β signal transduction. J Leukoc Biol. 71:731–740. 2002.PubMed/NCBI

10 

Amento EP and Beck LS: TGF-β and wound healing In Ciba Foundation Symposium 157 - Clinical Applications of TGF-β. John Wiley & Sons Ltd; Chichester: pp. 115–136. 1991

11 

Czuwara-Ladykowska J, Sementchenko VI, Watson DK and Trojanowska M: Ets1 is an effector of the transforming growth factor-β (TGF-β) signaling pathway and an antagonist of the profibrotic effects of TGF-β. J Biol Chem. 277:20399–20408. 2002. View Article : Google Scholar : PubMed/NCBI

12 

Verrecchia F and Mauviel A: Transforming growth factor-β signaling through the Smad pathway: Role in extracellular matrix gene expression and regulation. J Invest Dermatol. 118:211–215. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Kitano Y, Murazumi K, Duan J, Kurose K, Kobayashi S, Sugawara T and Hirata T: Effect of dietary porphyran from the red alga, Porphyra yezoensis, on glucose metabolism in diabetic KK-Ay mice. J Nutr Sci Vitaminol (Tokyo). 58:14–19. 2012. View Article : Google Scholar

14 

Jiang LF: The polysaccharides from porphyra yezoensis suppress the denaturation of bighead carp myofibrillar protein. Int J Biol Macromol. 68:18–20. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Kwon MJ and Nam TJ: Porphyran induces apoptosis related signal pathway in AGS gastric cancer cell lines. Life Sci. 79:1956–1962. 2006. View Article : Google Scholar : PubMed/NCBI

16 

Shin ES, Hwang HJ, Kim IH and Nam TJ: A glycoprotein from Porphyra yezoensis produces anti-inflammatory effects in liposaccharide-stimulated macrophages via the TLR4 signaling pathway. Int J Mol Med. 28:809–815. 2011.PubMed/NCBI

17 

Qian L, Zhou Y and Ma JX: Hypolipidemic effect of the polysaccharides from Porphyra yezoensis. Int J Biol Macromol. 68:48–49. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Ryu J, Park SJ, Kim IH, Choi YH and Nam TJ: Protective effect of porphyra-334 on UVA-induced photoaging in human skin fibroblasts. Int J Mol Med. 34:796–803. 2014.PubMed/NCBI

19 

Lee MK, Kim YM, Kim IH, Choi YH and Nam TJ: Pyropia yezoensis peptide protects against dexamethasone-induced muscle atrophy through the down-regulation Atrogin1/MAFbx and MuRF1 in mouse C2C12 myotubes. Mol Med Rep. In press.

20 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−ΔΔC(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

21 

Shoulders MD and Raines RT: Collagen structure and stability. Annu Rev Biochem. 78:929–958. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Ryhanen L and Uitto J: Elastic fibers of the connective tissue. Biochem Physiol Skin. 1:433–447. 1983.

23 

Sephel GC and Davidson JM: Elastin production in human skin fibroblast cultures and its decline with age. J Invest Dermatol. 86:279–285. 1986. View Article : Google Scholar : PubMed/NCBI

24 

Choi MS, Yoo MS, Son DJ, Jung HY, Lee SH, Jung JK, Lee BC, Yun YP, Pyo HB and Hong JT: Increase of collagen synthesis by obovatol through stimulation of the TGF-beta signaling and inhibition of matrix metalloproteinase in UVB-irradiated human fibroblast. J Dermatol Sci. 46:127–137. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Wierstra I: Sp1: emerging roles - beyond constitutive activation of TATA-less housekeeping genes. Biochem Biophys Res Commun. 372:1–13. 2008. View Article : Google Scholar : PubMed/NCBI

26 

Park JH, Kim SR, An HJ, Kim WJ, Choe M and Han JA: Esculetin promotes type I procollagen expression in human dermal fibroblasts through MAPK and PI3K/Akt pathways. Mol Cell Biochem. 368:61–67. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Zhang W, Ou J, Inagaki Y, Greenwel P and Ramirez F: Synergistic cooperation between Sp1 and Smad3/Smad4 mediates transforming growth factor β1 stimulation of α 2(I)-collagen (COL1A2) transcription. J Biol Chem. 275:39237–39245. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Kim YM, Jung HJ, Choi JS and Nam TJ: Anti-wrinkle effects of a tuna heart H2O fraction on Hs27 human fibroblasts. Int J Mol Med. 37:92–98. 2016.

29 

El-Domyati M, Attia S, Saleh F, Brown D, Birk DE, Gasparro F, Ahmad H and Uitto J: Intrinsic aging vs. photoaging: A comparative histopathological, immunohistochemical, and ultra-structural study of skin. Exp Dermatol. 11:398–405. 2002. View Article : Google Scholar : PubMed/NCBI

30 

Samarakoon K and Jeon YJ: Bio-functionalities of proteins derived from marine algae - A review. Food Res Int. 48:948–960. 2012. View Article : Google Scholar

31 

Varani J, Warner RL, Gharaee-Kermani M, Phan SH, Kang S, Chung JH, Wang ZQ, Datta SC, Fisher GJ and Voorhees JJ: Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin. J Invest Dermatol. 114:480–486. 2000. View Article : Google Scholar : PubMed/NCBI

32 

Leask A and Abraham DJ: TGF-β signaling and the fibrotic response. FASEB J. 18:816–827. 2004. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Kim C, Kim Y, Lee M, Kim I, Choi Y and Nam T: Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27. Int J Mol Med 39: 31-38, 2017.
APA
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., & Nam, T. (2017). Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27. International Journal of Molecular Medicine, 39, 31-38. https://doi.org/10.3892/ijmm.2016.2807
MLA
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., Nam, T."Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27". International Journal of Molecular Medicine 39.1 (2017): 31-38.
Chicago
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., Nam, T."Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27". International Journal of Molecular Medicine 39, no. 1 (2017): 31-38. https://doi.org/10.3892/ijmm.2016.2807
Copy and paste a formatted citation
x
Spandidos Publications style
Kim C, Kim Y, Lee M, Kim I, Choi Y and Nam T: Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27. Int J Mol Med 39: 31-38, 2017.
APA
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., & Nam, T. (2017). Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27. International Journal of Molecular Medicine, 39, 31-38. https://doi.org/10.3892/ijmm.2016.2807
MLA
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., Nam, T."Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27". International Journal of Molecular Medicine 39.1 (2017): 31-38.
Chicago
Kim, C., Kim, Y., Lee, M., Kim, I., Choi, Y., Nam, T."Pyropia yezoensis peptide promotes collagen synthesis by activating the TGF-β/Smad signaling pathway in the human dermal fibroblast cell line Hs27". International Journal of Molecular Medicine 39, no. 1 (2017): 31-38. https://doi.org/10.3892/ijmm.2016.2807
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team