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Article

SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells

  • Authors:
    • Lei Zhang
    • Wen-Hui Lou
    • Xue-Feng Xu
    • Wenchuan Wu
    • Ye-Fei Rong
    • Da-Yong Jin
  • View Affiliations / Copyright

    Affiliations: Pancreatic Cancer Group, General Surgery Department, Zhongshan Hospital, Fudan Universitiy, Shanghai 200232, P.R. China
  • Pages: 217-222
    |
    Published online on: November 22, 2016
       https://doi.org/10.3892/ijmm.2016.2810
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Abstract

The overexpression of interleukin-8 (IL-8) is closely associated with poor tumor differentiation, metastasis and tumor progression. This study aimed to examine the effects and mechanisms of action of SN38 (a metabolite of the camptothecin derivative, CPT-11) on IL-8 expression in HCT8 cells, using ELISA, CCK-8 and western blot analysis. Among jatrorrhizine, evodiamine, 5-fluorouracil and SN38, SN38 was found to inhibit the proliferation of HCT8 cells in a dose-dependent manner, but to increase IL-8 secretion from HCT8 cells. Of the other agents, evodiamine was found to inhibit both IL-8 secretion and cell proliferation, and jatrorrhizine was found to increase IL-8 secretion without any obvious inhibitory effect on cell proliferation. Further experiments revealed that the increased activation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK)1/2 and c-Jun N-terminal kinase (JNK) by SN38 contributed to the decreased cell proliferation and to the overexpression of IL-8 induced by SN38. Our results suggested that the MAPK pathways are activated by SN38, resulting in the upregulation of IL-8 expression and in the inhibition of cell proliferation in an IL-8-independent manner. Thus, the potential benefit of the use of a combination of camptothecin-11 with other chemical drugs with inhibitory effects on IL-8 expression, should be paid more attention in treating colon cancer.
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1 

Kuai WX, Wang Q, Yang XZ, Zhao Y, Yu R and Tang XJ: Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells. World J Gastroenterol. 18:979–985. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Bünger S, Haug U, Kelly FM, Klempt-Giessing K, Cartwright A, Posorski N, Dibbelt L, Fitzgerald SP, Bruch HP, Roblick UJ, et al BMBF-Consortium 'Colorectal Cancer Screening Chip': Toward standardized high-throughput serum diagnostics: Multiplex-protein array identifies IL-8 and VEGF as serum markers for colon cancer. J Biomol Screen. 16:1018–1026. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kitadai Y, Takahashi Y, Haruma K, Naka K, Sumii K, Yokozaki H, Yasui W, Mukaida N, Ohmoto Y, Kajiyama G, et al: Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice. Br J Cancer. 81:647–653. 1999. View Article : Google Scholar : PubMed/NCBI

4 

Kido S, Kitadai Y, Hattori N, Haruma K, Kido T, Ohta M, Tanaka S, Yoshihara M, Sumii K, Ohmoto Y and Chayama K: Interleukin 8 and vascular endothelial growth factor - prognostic factors in human gastric carcinomas? Eur J Cancer. 37:1482–1487. 2001. View Article : Google Scholar : PubMed/NCBI

5 

Xie K: Interleukin-8 and human cancer biology. Cytokine Growth Factor Rev. 12:375–391. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Matsuo Y, Ochi N, Sawai H, Yasuda A, Takahashi H, Funahashi H, Takeyama H, Tong Z and Guha S: CXCL8/IL-8 and CXCL12/SDF-1alpha co-operatively promote invasiveness and angiogenesis in pancreatic cancer. Int J Cancer. 124:853–861. 2009. View Article : Google Scholar :

7 

Ju D, Sun D, Xiu L, Meng X, Zhang C and Wei P: Interleukin-8 is associated with adhesion, migration and invasion in human gastric cancer SCG-7901 cells. Med Oncol. 29:91–99. 2012. View Article : Google Scholar

8 

Asfaha S, Dubeykovskiy AN, Tomita H, Yang X, Stokes S, Shibata W, Friedman RA, Ariyama H, Dubeykovskaya ZA, Muthupalani S, et al: Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis. Gastroenterology. 144:155–166. 2013. View Article : Google Scholar

9 

Shi HL, Wu XJ, Liu Y and Xie JQ: Berberine counteracts enhanced IL-8 expression of AGS cells induced by evodiamine. Life Sci. 93:830–839. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Li X, Zhao SJ, Shi HL, Qiu SP, Xie JQ, Wu H, Zhang BB, Wang ZT, Yuan JY and Wu XJ: Berberine hydrochloride IL-8 dependently inhibits invasion and IL-8-independently promotes cell apoptosis in MDA-MB-231 cells. Oncol Rep. 32:2777–2788. 2014.PubMed/NCBI

11 

Cacev T, Radosević S, Krizanac S and Kapitanović S: Influence of interleukin-8 and interleukin-10 on sporadic colon cancer development and progression. Carcinogenesis. 29:1572–1580. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Lee YS, Choi I, Ning Y, Kim NY, Khatchadourian V, Yang D, Chung HK, Choi D, LaBonte MJ, Ladner RD, et al: Interleukin-8 and its receptor CXCR2 in the tumour microenvironment promote colon cancer growth, progression and metastasis. Br J Cancer. 106:1833–1841. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Collins TS, Lee LF and Ting JP: Paclitaxel upregulates interleukin-8 synthesis in human lung carcinoma through an NF-kappaB- and AP-1-dependent mechanism. Cancer Immunol Immunother. 49:78–84. 2000. View Article : Google Scholar : PubMed/NCBI

14 

De Larco JE, Wuertz BR, Manivel JC and Furcht LT: Progression and enhancement of metastatic potential after exposure of tumor cells to chemotherapeutic agents. Cancer Res. 61:2857–2861. 2001.PubMed/NCBI

15 

Lev DC, Onn A, Melinkova VO, Miller C, Stone V, Ruiz M, McGary EC, Ananthaswamy HN, Price JE and Bar-Eli M: Exposure of melanoma cells to dacarbazine results in enhanced tumor growth and metastasis in vivo. J Clin Oncol. 22:2092–2100. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Kishida O, Miyazaki Y, Murayama Y, Ogasa M, Miyazaki T, Yamamoto T, Watabe K, Tsutsui S, Kiyohara T, Shimomura I, et al: Gefitinib (Iressa, ZD1839) inhibits SN38-triggered EGF signals and IL-8 production in gastric cancer cells. Cancer Chemother Pharmacol. 55:584–594. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Waugh DJ and Wilson C: The interleukin-8 pathway in cancer. Clin Cancer Res. 14:6735–6741. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Bleiberg H: CPT-11 in gastrointestinal cancer. Eur J Cancer. 35:371–379. 1999. View Article : Google Scholar : PubMed/NCBI

19 

Ajani JA, Baker J, Pisters PW, Ho L, Mansfield PF, Feig BW and Charnsangavej C: CPT-11 plus cisplatin in patients with advanced, untreated gastric or gastroesophageal junction carcinoma: Results of a phase II study. Cancer. 94:641–646. 2002. View Article : Google Scholar : PubMed/NCBI

20 

Britschgi A, Andraos R, Brinkhaus H, Klebba I, Romanet V, Müller U, Murakami M, Radimerski T and Bentires-Alj M: JAK2/STAT5 inhibition circumvents resistance to PI3K/mTOR blockade: A rationale for cotargeting these pathways in metastatic breast cancer. Cancer Cell. 22:796–811. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Zhang W, Feng M, Zheng G, Chen Y, Wang X, Pen B, Yin J, Yu Y and He Z: Chemoresistance to 5-fluorouracil induces epithelial-mesenchymal transition via upregulation of Snail in MCF7 human breast cancer cells. Biochem Biophys Res Commun. 417:679–685. 2012. View Article : Google Scholar

22 

Shao N, Chen LH, Ye RY, Lin Y and Wang SM: The depletion of interleukin-8 causes cell cycle arrest and increases the efficacy of docetaxel in breast cancer cells. Biochem Biophys Res Commun. 431:535–541. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Shi HL, Xie JQ and Wu DZ: Effect of berberine on cell proliferation and IL-8 expression in AGS cells. Pharmacol Clin Chin Mater Med Chin. 28:45–48. 2012.

24 

Itoh Y, Joh T, Tanida S, Sasaki M, Kataoka H, Itoh K, Oshima T, Ogasawara N, Togawa S, Wada T, et al: IL-8 promotes cell proliferation and migration through metalloproteinase-cleavage proHB-EGF in human colon carcinoma cells. Cytokine. 29:275–282. 2005.PubMed/NCBI

25 

Chelouche-Lev D, Miller CP, Tellez C, Ruiz M, Bar-Eli M and Price JE: Different signalling pathways regulate VEGF and IL-8 expression in breast cancer: Implications for therapy. Eur J Cancer. 40:2509–2518. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Maxwell PJ, Gallagher R, Seaton A, Wilson C, Scullin P, Pettigrew J, Stratford IJ, Williams KJ, Johnston PG and Waugh DJ: HIF-1 and NF-kappaB-mediated upregulation of CXCR1 and CXCR2 expression promotes cell survival in hypoxic prostate cancer cells. Oncogene. 26:7333–7345. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Bezzerri V, Borgatti M, Finotti A, Tamanini A, Gambari R and Cabrini G: Mapping the transcriptional machinery of the IL-8 gene in human bronchial epithelial cells. J Immunol. 187:6069–6081. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Ning Y and Lenz HJ: Targeting IL-8 in colorectal cancer. Expert Opin Ther Targets. 16:491–497. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Cao AL, Tang QF, Zhou WC, Qiu YY, Hu SJ and Yin PH: Ras-ERK signaling pathway is involved in curcumin-induced cell cycle arrest and apoptosis in human gastric carcinoma AGS cells. J Asian Nat Prod Res. 17:56–63. 2015. View Article : Google Scholar

30 

Park SY, Kim JY, Lee SM, Jun CH, Cho SB, Park CH, Joo YE, Kim HS, Choi SK and Rew JS: Capsaicin induces apoptosis and modulates MAPK signaling in human gastric cancer cells. Mol Med Rep. 9:499–502. 2014.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang L, Lou W, Xu X, Wu W, Rong Y and Jin D: SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells. Int J Mol Med 39: 217-222, 2017.
APA
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., & Jin, D. (2017). SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells. International Journal of Molecular Medicine, 39, 217-222. https://doi.org/10.3892/ijmm.2016.2810
MLA
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., Jin, D."SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells". International Journal of Molecular Medicine 39.1 (2017): 217-222.
Chicago
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., Jin, D."SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells". International Journal of Molecular Medicine 39, no. 1 (2017): 217-222. https://doi.org/10.3892/ijmm.2016.2810
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang L, Lou W, Xu X, Wu W, Rong Y and Jin D: SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells. Int J Mol Med 39: 217-222, 2017.
APA
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., & Jin, D. (2017). SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells. International Journal of Molecular Medicine, 39, 217-222. https://doi.org/10.3892/ijmm.2016.2810
MLA
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., Jin, D."SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells". International Journal of Molecular Medicine 39.1 (2017): 217-222.
Chicago
Zhang, L., Lou, W., Xu, X., Wu, W., Rong, Y., Jin, D."SN38 increases IL-8 expression through the MAPK pathways in HCT8 cells". International Journal of Molecular Medicine 39, no. 1 (2017): 217-222. https://doi.org/10.3892/ijmm.2016.2810
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