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Article

Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells

  • Authors:
    • Ge Sheng Cheng
    • Yu Shun Zhang
    • Ting Ting Zhang
    • Lu He
    • Xing Ye Wang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 223-230
    |
    Published online on: December 7, 2016
       https://doi.org/10.3892/ijmm.2016.2820
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Abstract

Pulmonary arterial hypertension (PAH) is a common clinical cardiovascular disease, leading to the excessive proliferation of pulmonary artery smooth muscle cells (PASMCs) and endothelial cells, and is associated with a high mortality rate. Recently, stem- and progenitor cell-mediated gene therapies have provided a novel approach for the treatment of PAH. However, the function of human bone marrow-derived mesenchymal stem cells (hBM‑MSCs) modified with the insulin-like growth factor binding protein-3 (IGFBP-3) gene in the regulation of PAH is not yet fully understood. In this study, we explored the biological role of IGFBP‑3-modified hBM‑MSCs in the proliferation of human PASMCs (hPASMCs), and also investigated the potential underlying molecular mechanisms. Our results revealed that IGFBP-3-modified hBM‑MSCs inhibited the proliferation of angiotensin II-stimulated hPASMCs following co-culture on cell culture inserts. In addition, total DNA synthesis and the protein levels of hPASMCs in co-culture were decreased. Moreover, the IGFBP‑3-modified hBM‑MSCs promoted apoptosis and downregulated the expression of B-cell lymphoma-2 (Bcl-2), but increased the expression of Bcl-2 associated X protein (Bax) in hPASMCs. Furthermore, the IGFBP‑3-modified hBM‑MSCs significantly induced a phenotypic change in the hPASMCs from the synthetic to the contractile phenotype in co-culture. Importantly, the levels of several related proteins in the hPASMCs, including phosphorylated (p-)insulin receptor substrate-1 (p-IRS-1), phosphoinositide 3-kinase (p-PI3K), serine/threonine-protein kinase (p-Akt), mitogen-activated protein kinase (p-p38), p-Jun N-terminal kinase (p-JNK) and extracellular signal-regulated kinase (p-ERK), were markedly decreased by the IGFBP-3-modified hBM‑MSCs following co-culture. Taken together, our findings suggest that IGFBP-3-modified hBM‑MSCs inhibit the proliferation and promote the apoptosis of hPASMCs, and promote the swithc to a contractile phenotype in more effectively than wild-type hBM‑MSCs, possibly through the activation of the PI3K/Akt and Ras-mitogen-activated protein kinase (MAPK) signaling pathways. The findings of our study suggest that IGFBP‑3‑modified hBM‑MSCs may be a promising therapeutic strategy for the treatment of PAH.
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1 

Ibrahim el-SH and Bajwa AA: Severe pulmonary arterial hypertension: Comprehensive evaluation by magnetic resonance imaging. Case Rep Radiol. 2015:946–920. 2015.

2 

Nogueira-Ferreira R, Vitorino R, Ferreira R and Henriques-Coelho T: Exploring the monocrotaline animal model for the study of pulmonary arterial hypertension: A network approach. Pulm Pharmacol Ther. 35:8–16. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Perrin S, Chaumais MC, O'Connell C, Amar D, Savale L, Jaïs X, Montani D, Humbert M, Simonneau G and Sitbon O: New pharmacotherapy options for pulmonary arterial hypertension. Expert Opin Pharmacother. 16:2113–2131. 2015. View Article : Google Scholar : PubMed/NCBI

4 

Weitzenblum E, Chaouat A, Canuet M and Kessler R: Pulmonary hypertension in chronic obstructive pulmonary disease and interstitial lung diseases. Semin Respir Crit Care Med. 30:458–470. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Xiao ZC and Liu YB: Treatment advance and tendency of pulmonary arterial hypertension. Clin Med Engineering. 23:257–260. 2016.In Chinese.

6 

Wang CH and An Y: Progress of stem cell treatment of pulmonary arterial hypertension. Chin J Clin Thorac Cardiovasc Surg. 23:294–298. 2016.In Chinese.

7 

Firth AL, Yao W, Ogawa A, Madani MM, Lin GY and Yuan JX: Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension. Am J Physiol Cell Physiol. 298:C1217–C1225. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Takemiya K, Kai H, Yasukawa H, Tahara N, Kato S and Imaizumi T: Mesenchymal stem cell-based prostacyclin synthase gene therapy for pulmonary hypertension rats. Basic Res Cardiol. 105:409–417. 2010. View Article : Google Scholar

9 

Bach LA: Insulin-like growth factor binding proteins - an update. Pediatr Endocrinol Rev. 13:521–530. 2015.

10 

Kielczewski JL, Jarajapu YP, McFarland EL, Cai J, Afzal A, Li Calzi S, Chang KH, Lydic T, Shaw LC, Busik J, et al: Insulin-like growth factor binding protein-3 mediates vascular repair by enhancing nitric oxide generation. Circ Res. 105:897–905. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Moser DR, Lowe WL Jr, Dake BL, Booth BA, Boes M, Clemmons DR and Bar RS: Endothelial cells express insulin-like growth factor-binding proteins 2 to 6. Mol Endocrinol. 6:1805–1814. 1992.PubMed/NCBI

12 

Johnson MA and Firth SM: IGFBP-3: A cell fate pivot in cancer and disease. Growth Horm IGF Res. 24:164–173. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Valentinis B, Bhala A, DeAngelis T, Baserga R and Cohen P: The human insulin-like growth factor (IGF) binding protein-3 inhibits the growth of fibroblasts with a targeted disruption of the IGF-I receptor gene. Mol Endocrinol. 9:361–367. 1995.PubMed/NCBI

14 

Lofqvist C, Chen J, Connor KM, Smith AC, Aderman CM, Liu N, Pintar JE, Ludwig T, Hellstrom A and Smith LE: IGFBP3 suppresses retinopathy through suppression of oxygen-induced vessel loss and promotion of vascular regrowth. Proc Natl Acad Sci USA. 104:10589–10594. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Tajsic T and Morrell NW: Smooth muscle cell hypertrophy, proliferation, migration and apoptosis in pulmonary hypertension. Compr Physiol. 1:295–317. 2011.PubMed/NCBI

16 

Chen PK, Shi B, Long XP, Liu ZJ, Wang ZL and Wang DM: Effects of rat mesenchymal stem cells modified by CGRP on proliferation and phenotype transformation of vascular smooth muscle cells in vitro. Chin J Pathophysiology. 29:1777–1782. 2013.In Chinese.

17 

Su XY, Jiang XM and Chen SL: The expression profile of IGFBP family in pulmonary artery smooth muscle cells of rats with pulmonary hypertension. Zhonghua Linchuang Yishi Zazhi. 9:1143–1148. 2015.In Chinese.

18 

Schinköthe T, Bloch W and Schmidt A: In vitro secreting profile of human mesenchymal stem cells. Stem Cells Dev. 17:199–206. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Firth SM, Ganeshprasad U and Baxter RC: Structural determinants of ligand and cell surface binding of insulin-like growth factor-binding protein-3. J Biol Chem. 273:2631–2638. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Xia Y, Bhattacharyya A, Roszell EE, Sandig M and Mequanint K: The role of endothelial cell-bound Jagged1 in Notch3-induced human coronary artery smooth muscle cell differentiation. Biomaterials. 33:2462–2472. 2012. View Article : Google Scholar

21 

Li Y, Liu G, Cai D, Pan B, Lin Y, Li X, Li S, Zhu L, Liao X and Wang H: H2S inhibition of chemical hypoxia-induced proliferation of HPASMCs is mediated by the upregulation of COX-2/PGI2. Int J Mol Med. 33:359–366. 2014.

22 

Liu Y, Tian HY, Yan XL, Fan FL, Wang WP, Han JL, Zhang JB, Ma Q, Meng Y and Wei F: Serotonin inhibits apoptosis of pulmonary artery smooth muscle cell by pERK1/2 and PDK through 5-HT1B receptors and 5-HT transporters. Cardiovasc Pathol. 22:451–457. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Squillaro T, Peluso G and Galderisi U: Clinical trials with mesenchymal stem cells: An update. Cell Transplant. 25:829–848. 2016. View Article : Google Scholar

24 

Baraniak PR and McDevitt TC: Stem cell paracrine actions and tissue regeneration. Regen Med. 5:121–143. 2010. View Article : Google Scholar :

25 

Chen JY, An R, Liu ZJ, Wang JJ, Chen SZ, Hong MM, Liu JH, Xiao MY and Chen YF: Therapeutic effects of mesenchymal stem cell-derived microvesicles on pulmonary arterial hypertension in rats. Acta Pharmacol Sin. 35:1121–1128. 2014. View Article : Google Scholar : PubMed/NCBI

26 

Agostini-Dreyer A, Jetzt AE, Stires H and Cohick WS: Endogenous IGFBP-3 mediates intrinsic apoptosis through modulation of Nur77 phosphorylation and nuclear export. Endocrinology. 156:4141–4151. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Muzumdar RH, Ma X, Fishman S, Yang X, Atzmon G, Vuguin P, Einstein FH, Hwang D, Cohen P and Barzilai N: Central and opposing effects of IGF-I and IGF-binding protein-3 on systemic insulin action. Diabetes. 55:2788–2796. 2006. View Article : Google Scholar : PubMed/NCBI

28 

Chan SS, Twigg SM, Firth SM and Baxter RC: Insulin-like growth factor binding protein-3 leads to insulin resistance in adipocytes. J Clin Endocrinol Metab. 90:6588–6595. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Chang RL, Lin JW, Hsieh DJ, Yeh YL, Shen CY, Day CH, Ho TJ, Viswanadha VP, Kuo WW and Huang CY: Long-term hypoxia exposure enhanced IGFBP-3 protein synthesis and secretion resulting in cell apoptosis in H9c2 myocardial cells. Growth Factors. 33:275–281. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Blouin MJ, Bazile M, Birman E, Zakikhani M, Florianova L, Aleynikova O, Powell DR and Pollak M: Germ line knockout of IGFBP-3 reveals influences of the gene on mammary gland neoplasia. Breast Cancer Res Treat. 149:577–585. 2015. View Article : Google Scholar : PubMed/NCBI

31 

Schermuly RT, Ghofrani HA, Wilkins MR and Grimminger F: Mechanisms of disease: Pulmonary arterial hypertension. Nat Rev Cardiol. 8:443–455. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Mandegar M, Fung YC, Huang W, Remillard CV, Rubin LJ and Yuan JX: Cellular and molecular mechanisms of pulmonary vascular remodeling: Role in the development of pulmonary hypertension. Microvasc Res. 68:75–103. 2004. View Article : Google Scholar : PubMed/NCBI

33 

Jeffery TK and Morrell NW: Molecular and cellular basis of pulmonary vascular remodeling in pulmonary hypertension. Prog Cardiovasc Dis. 45:173–202. 2002. View Article : Google Scholar

34 

Mohanraj L, Kim HS, Li W, Cai Q, Kim KE, Shin HJ, Lee YJ, Lee WJ, Kim JH and Oh Y: IGFBP-3 inhibits cytokine-induced insulin resistance and early manifestations of atherosclerosis. PLoS One. 8:e550842013. View Article : Google Scholar : PubMed/NCBI

35 

Wu J, Yu Z and Su D: BMP4 protects rat pulmonary arterial smooth muscle cells from apoptosis by PI3K/AKT/Smad1/5/8 signaling. Int J Mol Sci. 15:13738–13754. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Kiss T and Kovacs K, Komocsi A, Tornyos A, Zalan P, Sumegi B, Gallyas F Jr and Kovacs K: Novel mechanisms of sildenafil in pulmonary hypertension involving cytokines/chemokines, MAP kinases and Akt. PLoS One. 9:e1048902014. View Article : Google Scholar : PubMed/NCBI

37 

Garat CV, Crossno JT Jr, Sullivan TM, Reusch JE and Klemm DJ: Inhibition of phosphatidylinositol 3-kinase/Akt signaling attenuates hypoxia-induced pulmonary artery remodeling and suppresses CREB depletion in arterial smooth muscle cells. J Cardiovasc Pharmacol. 62:539–548. 2013. View Article : Google Scholar : PubMed/NCBI

38 

Biasin V, Chwalek K, Wilhelm J, Best J, Marsh LM, Ghanim B, Klepetko W, Fink L, Schermuly RT, Weissmann N, et al: Endothelin-1 driven proliferation of pulmonary arterial smooth muscle cells is c-fos dependent. Int J Biochem Cell Biol. 54:137–148. 2014. View Article : Google Scholar : PubMed/NCBI

39 

Mendivil A, Zhou C, Cantrell LA, Gehrig PA, Malloy KM, Blok LJ, Burger CW and Bae-Jump VL: AMG 479, a novel IGF-1-R antibody, inhibits endometrial cancer cell proliferation through disruption of the PI3K/Akt and MAPK pathways. Reprod Sci. 18:832–841. 2011. View Article : Google Scholar : PubMed/NCBI

40 

Zha Z, Zhang QH, Jiang ZX, Chen L, Lin H and Liang XM: Effect of angiotensin II on pregnancy-associated plasma protein A and insulin-like growth factor 1 gene expression in human umbilical artery smooth muscle cells. Nan Fang Yi Ke Da Xue Xue Bao. 29:195–198. 2009.In Chinese. PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Cheng GS, Zhang YS, Zhang TT, He L and Wang XY: Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. Int J Mol Med 39: 223-230, 2017.
APA
Cheng, G.S., Zhang, Y.S., Zhang, T.T., He, L., & Wang, X.Y. (2017). Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. International Journal of Molecular Medicine, 39, 223-230. https://doi.org/10.3892/ijmm.2016.2820
MLA
Cheng, G. S., Zhang, Y. S., Zhang, T. T., He, L., Wang, X. Y."Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells". International Journal of Molecular Medicine 39.1 (2017): 223-230.
Chicago
Cheng, G. S., Zhang, Y. S., Zhang, T. T., He, L., Wang, X. Y."Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells". International Journal of Molecular Medicine 39, no. 1 (2017): 223-230. https://doi.org/10.3892/ijmm.2016.2820
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng GS, Zhang YS, Zhang TT, He L and Wang XY: Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. Int J Mol Med 39: 223-230, 2017.
APA
Cheng, G.S., Zhang, Y.S., Zhang, T.T., He, L., & Wang, X.Y. (2017). Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. International Journal of Molecular Medicine, 39, 223-230. https://doi.org/10.3892/ijmm.2016.2820
MLA
Cheng, G. S., Zhang, Y. S., Zhang, T. T., He, L., Wang, X. Y."Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells". International Journal of Molecular Medicine 39.1 (2017): 223-230.
Chicago
Cheng, G. S., Zhang, Y. S., Zhang, T. T., He, L., Wang, X. Y."Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells". International Journal of Molecular Medicine 39, no. 1 (2017): 223-230. https://doi.org/10.3892/ijmm.2016.2820
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